Follitropin binding to receptors in testis: Studies on the reversibility and thermodynamics of the reaction

Andersen, Thomas T.; Curatolo, L M; Reichert, Leo E.

doi:10.1016/0303-7207(83)90055-2

Cited by 35 publications

(6 citation statements)

References 23 publications

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“…Ovarian weight in HCG-primed rats: Using the adopted method [22]: Receptor binding assay for FSH: Membrane preparations from bovine testes are used according to the methods of Cheng [23] and Andersen [24], and follow up the adopted method [22].…”

Section: Pharmacological Screeningmentioning

confidence: 99%

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Follicle-stimulating hormone (FSH) activities of some synthesized isoxazole and pyrazoline derivatives

Fayed¹,

Bahshwan²,

Abde-Megeid³

et al. 2018

Biomed Res

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Section: Pharmacological Screeningmentioning

confidence: 99%

“…Several receptors binding assay procedures have been described, e.g. Cheng [23], Andersen [24] using bovine testes and Reichert [22,25] was using rat testes tubule tissue. So this method calculate the specific binding percentage of each of the tested compounds to its receptor and given in Table 3.…”

Section: Pharmacological Screeningmentioning

confidence: 99%

Follicle-stimulating hormone (FSH) activities of some synthesized isoxazole and pyrazoline derivatives

Fayed¹,

Bahshwan²,

Abde-Megeid³

et al. 2018

Biomed Res

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“…Internalization rate of the receptor has never been measured directly by measuring the receptor itself. In addition, the reversibility of FSH binding decreases with increasing incubation time (Andersen, Curatolo, & Reichert, 1983). Measuring internalization under equilibrium conditions provides an estimate of internalization rate that is undoubtedly affected by recycling and degradation, and could in essence be considered a beta phase internalization rate.…”

Section: Phosphorylation Internalization and Recycling Of The Fsmentioning

confidence: 99%

Trafficking of the Follitropin Receptor

Ulloa‐Aguirre

Dias

Bousfield

et al. 2013

Methods in Enzymology

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The follitropin or follicle-stimulating hormone receptor (FSHR) belongs to a highly conserved subfamily of the G protein-coupled receptor (GPCR) superfamily and is mainly expressed in specific cells in the gonads. As any other GPCR, the newly synthesized FSHR has to be correctly folded and processed in order to traffic to the cell surface plasma membrane and interact with its cognate ligand. In this chapter, we describe in detail the conditions and procedures used to study outward trafficking of the FSHR from the endoplasmic reticulum to the plasma membrane. We also describe some methods to analyze phosphorylation, β-arrestin recruitment, internalization, and recycling of this particular receptor, which have proved useful in our hands for dissecting its downward trafficking and fate following agonist stimulation.

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“…Interestingly, these underexpressed hormones include FSH, whose serum levels normally increased in post-menopausal women [100,101]. It has been speculated that this decrease indicates increased binding of FSH to its receptors, which is irreversible at body temperature and results in permanent removal from the circulation [102]. Other protein biomarkers underexpressed in ovarian cancer include ApoA1 and transthyretin (TTR), which have been evaluated extensively as components of diagnostic panels for ovarian cancer and may originate from tumoral, local or systemic sources [62,68,103,104].…”

Section: Approaches and Controversies In Biologically Based Feature Selmentioning

confidence: 99%

Protein Biomarkers of Ovarian Cancer: The Forest and The Trees

Nolen

Lokshin

2011

Future Oncol.

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The goal of effective population-based screening for ovarian cancer remains elusive despite intense efforts aimed at improving upon biomarker and imaging modalities. While dozens of potential serum biomarkers for ovarian cancer have been identified in recent years, none have yet overcome the limitations that have hindered the clinical use of CA-125. Avenues of opportunity in biomarker development are emerging as investigators are beginning to appreciate the significance of remote, as well as local or regional, sources of biomarkers in the construction of diagnostic panels, as well as the importance of evaluating biomarkers in prediagnostic settings. As the list of candidate biomarkers of ovarian cancer continues to grow, refinements in the methods through which specific proteins are selected for further development as components of diagnostic panels are desperately sought. Such refinements must take into account both the bioinformatic and biological significance of each candidate. Approaches incorporating these considerations may potentially overcome the challenges to early detection posed by the histological heterogeneity of ovarian cancer. Here, we review the recent progress achieved in efforts to develop diagnostic biomarker panels for ovarian cancer and discuss the challenges that remain.

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Follitropin binding to receptors in testis: Studies on the reversibility and thermodynamics of the reaction

Cited by 35 publications

References 23 publications

Follicle-stimulating hormone (FSH) activities of some synthesized isoxazole and pyrazoline derivatives

Follicle-stimulating hormone (FSH) activities of some synthesized isoxazole and pyrazoline derivatives

Trafficking of the Follitropin Receptor

Protein Biomarkers of Ovarian Cancer: The Forest and The Trees

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