Follicular Nerve Evoked Responses in the Neonatal Rat Barrel Cortex

Nasretdinov, Azat; Akhmetshina, Dinara; Valeeva, Guzel; Khazipov, Roustem

doi:10.1007/s12668-016-0245-6

Cited by 1 publication

(3 citation statements)

References 4 publications

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“…In accordance with previous studies, LFP activity at P3-5 was discontinuous and dominated by early sharp waves (eSPWs; Fig. 6A-B) (Valeeva et al, 2019;Gainutdinov et al, 2023). Less consistently, we also detected short-lasting LFP oscillations in the theta-to-beta frequency range in several of the analyzed mice (Fig.…”

Section: Presynaptic Gi/o Signaling Is An Actuator Of Immature Hippoc...supporting

confidence: 93%

“…Network bursts in the developing hippocampus are generated in a relatively all-or-none manner (Prida and Sanchez-Andres, 1999;Gainutdinov et al, 2023). Theoretical work suggests that this behavior arises from a transient, unstable network state evoked by supra-threshold excitatory input, leading to a profound amplification of neuronal firing rates (Rahmati et al, 2017).…”

Section: Generative Mechanisms Of Network Bursts In the Developing Ca1mentioning

confidence: 99%

“…In CA1 during the first and second postnatal week, activity is characterized by intermittent network bursts that alternate with periods of low activity. Network bursts are driven by input from the sensory periphery (Mohns and Blumberg, 2010;Valeeva et al, 2019;Chen et al, 2023;Gainutdinov et al, 2023;Kostka and Hanganu-Opatz, 2023), but they can also be internally generated within the entorhinal-hippocampal formation (Ben-Ari et al, 1989;Leinekugel et al, 2002;Graf et al, 2021). By co-activating large numbers of neurons (Dard et al, 2022;Graf et al, 2022), network bursts were proposed to inform nascent CA1 circuits about the fundamental statistical properties of the external (environment) and internal (body) world, thereby preparing them for later cognitive and sensory demands (for review see Cossart and Khazipov, 2022;Kirmse and Zhang, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Chemogenetic silencing reveals presynaptic Gi/o protein-mediated inhibition of synchronized activity in the developing hippocampusin vivo

Graf,

Samiee,

Flossmann

et al. 2024

Preprint

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The development of neuronal circuits is an activity-dependent process. Research aimed at deciphering the learning rules governing these developmental refinements is increasingly utilizing a class of chemogenetic tools that employ Gi/o protein signaling (Gi-DREADDs) for neuronal silencing. However, their mechanisms of action and inhibitory efficacy in immature neurons with incompletely developed Gi/o signaling are poorly understood. Here, we analyze the impact of Gi/o signaling on cellular and network excitability in the neonatal hippocampus by expressing the Gi-DREADD hM4Di in telencephalic glutamatergic neurons of neonatal mice of both sexes. Using acousto-optic two-photon Ca2+ imaging, we report that activation of hM4Di leads to a complete arrest of spontaneous synchronized activity in CA1 in vitro. Electrophysiological analyses demonstrate that hM4Di-mediated silencing is not accounted for by changes in intrinsic excitability of CA1 pyramidal cells (PCs). Instead, activation of hM4Di robustly restrains synaptic glutamate release by the first postnatal week, effectively reducing recurrent excitation in CA1. In vivo, inhibition through hM4Di potently suppresses early sharp waves (eSPWs) and discontinuous oscillatory network activity in CA1 of head-fixed mice before eye opening. In summary, hM4Di dampens glutamatergic neurotransmission through a presynaptic mechanism sufficient to terminate spontaneous synchronized activity in the neonatal CA1. Our findings have implications for designing and interpreting experiments utilizing Gi-DREADDs in immature neurons and further point to a potential role of Gi/o-dependent endogenous neuromodulators in activity-dependent hippocampal development.

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Section: Presynaptic Gi/o Signaling Is An Actuator Of Immature Hippoc...supporting

confidence: 93%

Section: Generative Mechanisms Of Network Bursts In the Developing Ca1mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chemogenetic silencing reveals presynaptic Gi/o protein-mediated inhibition of synchronized activity in the developing hippocampusin vivo

Graf,

Samiee,

Flossmann

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Follicular Nerve Evoked Responses in the Neonatal Rat Barrel Cortex

Cited by 1 publication

References 4 publications

Chemogenetic silencing reveals presynaptic Gi/o protein-mediated inhibition of synchronized activity in the developing hippocampusin vivo

Chemogenetic silencing reveals presynaptic Gi/o protein-mediated inhibition of synchronized activity in the developing hippocampusin vivo

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