Follicular fluid is not a compulsory carrier of the oocyte at ovulation in the mare

Palmer, Eric; Duchamp, Guy; Cribiu, E.P.; Mahla, Rachid; Boyazoglu, S.; Bézard, J.

doi:10.1111/j.2042-3306.1997.tb05094.x

Cited by 11 publications

(3 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An associated possible scenario is that the cumulus does not attach to the follicle wall, and the transferred COCs are free floating in the follicle; this possibility is supported by the finding that all oocytes recovered in Experiment 1 were found in the initial aspiration of follicular fluid, rather than in the fluid from follicular flushing. As most follicular fluid appears to be lost into the peritoneum at ovulation in the mare [24] and the (native) COC appears to leave the follicle after most fluid has been expelled [25], it is possible that transferred free-floating COCs could exit with the initial fluid and be lost into the peritoneum. Decreased cumulus size may also hinder capture of the oocyte by the oviduct during ovulation.…”

Section: Discussionmentioning

confidence: 99%

Intrafollicular oocyte transfer in the horse: effect of autologous vs. allogeneic transfer and time of administration of ovulatory stimulus before transfer

Andino

Brom-de-Luna

Canesin

et al. 2019

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To assess meiotic and developmental competence after transfer of immature cumulus-oocyte complexes (COCs) to the preovulatory follicles of mares (intrafollicular oocyte transfer (IFOT)). Methods In Experiment 1, mares received an ovulatory stimulus at IFOT. Thirty hours later, COCs were recovered from the follicle, and mature oocytes underwent ICSI and embryo culture. In Experiments 2 and 3, autologous vs. allogeneic COCs were used. The mares were inseminated and embryos were recovered. In Experiment 3, the ovulatory stimulus was administered 9 h (autologous) and 15 h (allogeneic) before IFOT. In Experiment 4, only allogeneic COCs were used; the ovulatory stimulus was administered 9 or 15 h before IFOT. Excess embryos (autologous) and parentage-verified embryos (allogeneic) were considered IFOT-derived. Results In Experiment 1, 36/54 IFOT oocytes (67%) were recovered, of which 56% were mature, vs. 49% of in vitro matured oocytes (P > 0.1). After ICSI, blastocyst rates were 25% and 18%, respectively (P > 0.1). In Experiment 2, 0/6 autologous and 2/6 allogeneic IFOT yielded IFOT-derived embryos. In Experiment 3, 0/7 autologous and 2/5 allogeneic IFOT yielded IFOT-derived embryos. The proportion of mares yielding IFOT-derived embryos was lower after autologous vs. allogeneic IFOT (0/13 vs. 4/11; P < 0.05). In Experiment 4, 1/8 9-h and 1/7 15-h IFOT yielded IFOT-derived embryos. Conclusions Transferred oocytes mature within the follicle and can maintain developmental competence. Allogeneic IFOT was more efficient than was autologous IFOT. The time of ovulatory stimulation did not affect embryo yield. The IFOT procedure is still not repeatable enough to be recommended for clinical use.

show abstract

Section: Discussionmentioning

confidence: 99%

Intrafollicular oocyte transfer in the horse: effect of autologous vs. allogeneic transfer and time of administration of ovulatory stimulus before transfer

Andino

Brom-de-Luna

Canesin

et al. 2019

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…In a study on horses using the AFF technique, experimental results showed that the recovered oocytes were all present in the initial follicular aspiration fluid, not in the follicular flushing fluid [ 11 ]. During ovulation in mares, most of the follicular fluid seems to be lost into the peritoneum [ 81 ]. Therefore, transferred free–floating COCs may be lost into the peritoneum along with the initial fluid.…”

Section: Potential Negative Factors Influencing the Success Rate Of Affmentioning

confidence: 99%

Allogenic Follicular Fosterage Technology: Problems, Progress and Potential

Teng,

Zhao,

et al. 2024

Veterinary Sciences

View full text Add to dashboard Cite

The allogeneic follicular fosterage (AFF) technique transfers cumulus–oocyte complexes (COCs) from pubertal female animals to the dominant follicles of adult female animals for further development, allowing the COCs to further develop in a completely in vivo environment. This article reviews the history of AFF and JIVET and their effects on oocyte and embryo development as well as freezing resistance. Improving the efficiency and reproducibility of AFF technology is crucial to its clinical application. This article discusses factors that affect the success rate of AFF, including differences in specific technical procedures and differences between pubertal and adult follicles. Designing standardized procedures and details to improve the synchronization of donor COCs and recipient follicle maturity and reducing the damage to COCs caused by follicular aspiration may be the direction for improving the success rate of AFF in the future.

show abstract

“…Because of the particular structure of the equine ovary and its ovulation fossa, and because of the large follicular size, experimental sampling of follicular fluids throughout the cycle can be performed to study follicular maturation, without rupturing the follicle [ 270 ]. Complete removal of follicular fluid from a preovulatory follicle was used to demonstrate that follicular fluid was not necessary for ovulation [ 271 ]. The same approach enables the injection into the follicle of experimental compounds, immature oocytes to perform an “ex vivo maturation” [ 272 ], or spermatozoa.…”

Section: Where Is the Mare A Good Model For Art In Humans?mentioning

confidence: 99%

The Mare: A Pertinent Model for Human Assisted Reproductive Technologies?

Benammar

Derisoud

Vialard

et al. 2021

Animals

View full text Add to dashboard Cite

Although there are large differences between horses and humans for reproductive anatomy, follicular dynamics, mono-ovulation, and embryo development kinetics until the blastocyst stage are similar. In contrast to humans, however, horses are seasonal animals and do not have a menstrual cycle. Moreover, horse implantation takes place 30 days later than in humans. In terms of artificial reproduction techniques (ART), oocytes are generally matured in vitro in horses because ovarian stimulation remains inefficient. This allows the collection of oocytes without hormonal treatments. In humans, in vivo matured oocytes are collected after ovarian stimulation. Subsequently, only intra-cytoplasmic sperm injection (ICSI) is performed in horses to produce embryos, whereas both in vitro fertilization and ICSI are applied in humans. Embryos are transferred only as blastocysts in horses. In contrast, four cells to blastocyst stage embryos are transferred in humans. Embryo and oocyte cryopreservation has been mastered in humans, but not completely in horses. Finally, both species share infertility concerns due to ageing and obesity. Thus, reciprocal knowledge could be gained through the comparative study of ART and infertility treatments both in woman and mare, even though the horse could not be used as a single model for human ART.

show abstract

Follicular fluid is not a compulsory carrier of the oocyte at ovulation in the mare

Cited by 11 publications

References 6 publications

Intrafollicular oocyte transfer in the horse: effect of autologous vs. allogeneic transfer and time of administration of ovulatory stimulus before transfer

Intrafollicular oocyte transfer in the horse: effect of autologous vs. allogeneic transfer and time of administration of ovulatory stimulus before transfer

Allogenic Follicular Fosterage Technology: Problems, Progress and Potential

The Mare: A Pertinent Model for Human Assisted Reproductive Technologies?

Contact Info

Product

Resources

About