Follicular fluid contents of hyaluronic acid, follicle-stimulating hormone and steroids relative to the success of in vitro fertilization of human oocytes

Suchanek, Ernest; Šimunić, Velimir; Juretić, Dubravka; Grizelj, V.

doi:10.1016/s0015-0282(16)56890-3

Cited by 64 publications

(29 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These physicochemical properties contribute to its postulated roles in the extracellular matrix. HA has also been found in human ovarian follicular fluid, particularly in human chorionic gonadotropinstimulated ovaries (Suchanek et al 1994). Our measurements also confirmed the presence of HA in human follicular fluid derived from patients undergoing an in vitro fertilization embryo transfer (IVF-ET) protocol (Kobayashi et al 1998, unpublished data).…”

Section: Introductionsupporting

confidence: 81%

Immunolocalization of hyaluronic acid and inter-alpha-trypsin inhibitor in mice

Kobayashi

Sun

Terao

1999

Cell and Tissue Research

View full text Add to dashboard Cite

The direct interaction of hyaluronic acid (HA) and heavy chain (HC) of the inter-alpha-trypsin inhibitor (IalphaI) family plays a critical role in the organization and stabilization of the extracellular matrix. The aim of the present investigation was to elucidate the distribution of the IalphaI HC and HA in adult mouse tissues. An immunohistochemical method using a rabbit polyclonal antibody raised against mouse IalphaI heavy-chain peptide and a specific probe for HA (biotinylated HA-binding protein) was used to demonstrate an immunolocalization of IalphaI HC and HA. Distribution and localization of HA was of three types, namely, colocalization with IalphaI HC itself (cartilaginous tissue and ovary), localization around IalphaI HC immunostaining (lung, intestine and skeletal muscle), and localization at a small distance from IalphaI HC or a different distribution pattern (brain, liver, skin and kidney). These results indicate that IalphaI HC could function as an HA-rich matrix stabilizer on the cells of cartilage and maturing ovary, in which IalphaI HC shows colocalization with its predominant ligand, HA.

show abstract

Section: Introductionsupporting

confidence: 81%

Immunolocalization of hyaluronic acid and inter-alpha-trypsin inhibitor in mice

Kobayashi

Sun

Terao

1999

Cell and Tissue Research

View full text Add to dashboard Cite

show abstract

“…Hyaluronan is one of the most abundant substances in follicular, oviduct, and uterine fluid [17,34]. It is involved in several processes mediated through interaction with matrix components, cell proliferation, and cell differentiation [35].…”

Section: Discussionmentioning

confidence: 99%

In vitro bovine embryo production in a synthetic medium: Embryo development, cryosurvival, and establishment of pregnancy

et al. 2015

View full text Add to dashboard Cite

a b s t r a c tThe aim of this study was to develop an in vitro embryo culture medium without either fetal calf serum or BSA, using various growth factors and cytokines (GFs-CYKs; IGF-I, IGF-II, bFGF, LIF, GM-CSF, TGF-b1, and PDGF-BB), and other molecules with surfactant and embryotrophic properties, such as recombinant albumin (RA) and hyaluronan (HA). The first part of the study was dedicated to define the best combination of GFs-CYKs þ RA þ HA for optimal embryonic development. Next, we compared development rates and embryo quality (inner cell mass [ICM]-to-total cell number [TCN] ratio), and postthaw survival and hatching rates using this synthetic medium (T1) and a control medium: synthetic oviduct fluid þ BSA þ ITS (insulin, transferrin, and selenium). The blastocyst rates were significantly higher with T1 than those with the control at 7 and 8 days after fertilization. There was no significant difference in TCN or the ICM/TCN ratio between the two treatments. Survival and hatching rates 48 hours after thawing were similar for both treatments. Finally, nine embryo transfers were conducted using fresh and previously frozen Day-7 blastocysts to evaluate the in vivo viability of embryos produced in this synthetic medium; four gestations were obtained from six fresh embryos and one gestation from three frozen embryos. In conclusion, the fetal calf serum and BSA-free medium, supplemented with GFs-CYKs þ RA þ HA, improved embryo development and gave comparable ICM/TCN ratios and postthaw survival rates to the control with BSA. Fresh and frozen embryos produced in this medium are viable for embryo transfer. This fully synthetic method of embryo culture is a useful means of reducing the risk of disease transmission via embryo transfer.

show abstract

“…Another pair of proteins (c-kit and its ligand KL) also appear to be relevant for the control of oocyte maturation since a recent report [52] [6,13,36] although some other studies failed to confirm this finding [92,94,116] [55]. Interestingly, a recent report described a positive effect of inhibin or activin on cytoplasmic maturation [90].…”

Section: Introductionmentioning

confidence: 99%