Follicular fluid and serum concentrations of myo-inositol in patients undergoing IVF: relationship with oocyte quality

Chiu, Tony Tak Yu; Rogers, Michael S.; Law, Eric; Briton-Jones, Christine; Cheung, L.P.; Haines, Christopher J.

doi:10.1093/humrep/17.6.1591

Cited by 148 publications

(111 citation statements)

References 42 publications

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“…The concentration of myo-inositol has been determined in FF by enzymatic assays (19), and it was found that the myo-inositol concentration is larger in the FF of good quality oocytes than of low quality ones. However, in the present work, only a weak signal from myo-inositol was detected in the J-resolved spectra, suggesting that it is present at low concentration.…”

Section: Discussionmentioning

confidence: 99%

“…Steroids (progesterone, 17-oestradiol-b, androstenedione) (13), gonadotropins, prolactin (14) or growth factors (16) have been particularly studied. In addition, the role of myo-inositol in follicular maturity (19) and of N-acylethanolamines, which have been found in human reproductive fluids, has been of particular interest (20). These substances may modulate follicular maturation and ovulation, normal and pathological ovarian function, placental and foetal physiology, lactation, infant physiology and behaviour, and may help to regulate multiple physiological and pathological processes in the reproductive system (20).…”

Section: Introductionmentioning

confidence: 99%

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NMR metabolic profile of human follicular fluid

et al. 2010

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The environment of the oocyte during its in vivo maturation consists of follicular fluid (FF) and is surrounded by granulosa cells. The FF is derived from the sanguineous plasma and secretions, synthesised in the follicle wall, that contain a large variety of growth factors, cytokines, amino acids, and other metabolites. These metabolites are presumably involved in the physiology of the oocyte. The identification, quantification and study of FF metabolites can provide additional information about the oocyte state which can be helpful in distinguishing those oocytes that have a greater capacity to be fertilised and to develop properly. The aim of this work is to identify the metabolic profile of FF samples exhaustively using High Resolution Nuclear Magnetic Resonance (NMR). A total of 30 FF samples from oocyte donors (<35 years) were analysed. Different monodimensional (1D) and bidimensional (2D) (homo and heteronuclear) NMR experiments were acquired. A total of 131 chemical shifts were assigned and 42 metabolites, including as example glucose, lactate, acetate, acetoacetate, pyruvate and b-hydroxybutyrate, were identified. High correlations were found between these important intermediaries of the energetic metabolic pathways of the follicle which can indicate the importance of these pathways in oocyte development. Some of these identified metabolites might be useful as biomarkers of the follicular maturation state, allowing oocytes with a higher fertilisation potential to be selected, thereby increasing pregnancy rates in women following in vitro fertilisation (IVF) treatments.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

NMR metabolic profile of human follicular fluid

et al. 2010

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“…MI is present in human follicular fluid, where elevated concentrations appear to play a positive role in follicular maturity and provide a marker of goodquality oocytes [9] . Our study demonstrated that MI oral supplementation restores spontaneous ovulation and menstrual cycles, and increases progesterone secretion in theluteal phase, in most infertile patients with PCOS.…”

Section: Discussionmentioning

confidence: 99%

Use of Myo-inositol for Ovulation Induction in Patients with Polycystic Ovary Syndrome

Ahmed¹

2017

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“…Foliküler sıvıdaki MI düzeyinin yüksek olması, daha yüksek kalitede bir oosit oluşumu ile korelasyon göster-mektedir. 12 İnositolün oosit kalitesi üzerine etkisini gösteren çalışmalar Tablo 1'de görülmektedir. İntrasitoplazmik sperm enjeksiyonu [intracytoplasmic sperm injection (ICSI)] uygulanan ve olumlu sonuç vermeyen bireylerde MI'nın over fonksiyonu üzerine etkisini değerlendirmek amacıyla yapılan çalışmada, ICSI programına daha önce dâhil edilmiş, ancak başarı elde edilememiş 72 birey iki farklı gruba ayrılmıştır.…”

Section: Ovülasyonunclassified