Follicular fluid and mural granulosa cells microRNA profiles vary in in vitro fertilization patients depending on their age and oocyte maturation stage

Moreno, Juan Manuel; Núñez, Marı́a José; Quiñonero, Alicia; Martínez, Sebastián; Orden, Marina de la; Simón, Carlos; Pellicer, António; Díaz-García, César; Domı́nguez, Francisco

doi:10.1016/j.fertnstert.2015.07.001

Cited by 59 publications

(47 citation statements)

References 59 publications

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“…Prolonged human lifespan and postponed childbearing prompt researchers to investigate mechanisms underlying ovarian aging. Extensive studies have focused on characterizing mRNA and miRNAs profiles in human GCs according to maternal age [10, 15], and expanded our knowledge of molecular processes occurring in follicular cells. However, the application of new sequencing technologies provides opportunities to study other novel molecules with promising prospects.…”

Section: Discussionmentioning

confidence: 99%

“…GCs were individually purified from FF samples with similar methods as previously described [10, 34]. Briefly, FF sample of each person was centrifuged and resuspended in phosphate-buffered saline (PBS).…”

Section: Methodsmentioning

confidence: 99%

“…Since GCs provide an essential follicular micro-environment that influences oocyte competence and early embryo development [10–12], many studies have tried to use the transcriptome of GCs as a non-invasive approach to gain insight into micro-environment surrounding oocytes and predict oocyte developmental potential [13–16]. However, there is a lack of overlap among different studies possibly owing to population heterogeneity and the instability of linear RNAs [15].…”

Section: Introductionmentioning

confidence: 99%

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Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

et al. 2017

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Circular RNAs (circRNAs) are a unique class of endogenous RNAs which could be used as potential diagnostic and prognostic biomarkers of many diseases. Our study aimed to investigate circRNA profiles in human granulosa cells (GCs) during maternal aging and to uncover age-related circRNA variations that potentially reflect decreased oocyte competence. CircRNAs in GCs from in vitro fertilization (IVF) patients with young age (YA, ≤ 30 years) and advanced age (AA, ≥ 38 years) were profiled by microarray, and validated in 20 paired samples. The correlation between circRNAs expression and clinical characteristics was analyzed in additional 80 samples. Chip-based analysis revealed 46 up-regulated and 11 down-regulated circRNAs in AA samples (fold change > 2.0). Specifically, circRNA_103829, circRNA_103827 and circRNA_104816 were validated to be up-regulated, while circRNA_101889 was down-regulated in AA samples. After adjustment for gonadotropin treatment, only circRNA_103827 and circRNA_104816 levels were positively associated with maternal age (partial r = 0.332, P = 0.045; partial r = 0.473, P = 0.003; respectively). Moreover, circRNA_103827 and circRNA_104816 expressions in GCs were negatively correlated with the number of top quality embryos (r = -0.235, P = 0.036; r = -0.221, P = 0.049; respectively). Receiver operating characteristic (ROC) curve analysis indicated that the performance of circRNA_103827 for live birth prediction reached 0.698 [0.570–0.825], with 77.2% sensitivity and 60.9% specificity (P = 0.006), and that of circRNA_104816 was 0.645 [0.507–0.783] (P = 0.043). Bioinformatics analysis revealed that both circRNAs were potentially involved in glucose metabolism, mitotic cell cycle, and ovarian steroidogenesis. Therefore, age-related up-regulation of circRNA_103827 and circRNA_104816 might be potential indicators of compromised follicular micro-environment which could be used to predict IVF prognosis, and improve female infertility management.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

et al. 2017

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“…In addition to that, miRNAs can be secreted to the extracellular space [4] and have a role in intercellular communication in a hormone-like pattern between different cell types [5]. Therefore, miRNAs play an essential role in ovarian follicle development [6, 7], although little is known about the how miRNAs are regulated during the ovarian aging process [8, 9]. …”

Section: Introductionmentioning

confidence: 99%

“…In the female mouse the size of the ovarian reserve reduces 10 fold from six to 18 months of age [12]. Some miRNAs are known to be involved in premature ovarian failure in the rat [13] and were observed to be differentially regulated in the ovaries of ageing women [8, 9]. This evidence suggests that there is an association between miRNA expression and regulation of ovarian aging.…”

Section: Introductionmentioning

confidence: 99%

Changes of Ovarian microRNA Profile in Long-Living Ames Dwarf Mice during Aging

et al. 2017

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The Ames dwarf (df/df) mice have extended longevity and can preserve the ovarian reserve longer than Normal (N) mice. Based on this, the aim of our study was to evaluate the ovarian microRNA (miRNA) profile in young and aged df/df and N mice. Ovarian tissue was collected at 5–6 months and at 21–22 months of age for miRNA sequencing. We detected a total of 404 miRNAs in the ovarian samples, from which the abundance of 22 and 33 miRNAs changed with age in N and df/df mice, respectively. Of these, only three miRNAs were commonly regulated with age between N and df/df mice, indicating a very divergent miRNA profile between genotypes. We also detected that 46 miRNAs were regulated between N and df/df mice, of which 23 were regulated exclusively in young mice, 12 exclusively in old mice and 12 commonly regulated at young and old ages. Many genes likely to be targeted by these miRNAs are involved in the FoxO, mTOR, PI3k/Akt and insulin signaling pathways. These results suggest that the aging process has a differential impact on the ovarian miRNA profile in df/df mice, and suggest that these miRNAs can be central players in the maintenance of a younger ovarian phenotype.

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Polycystic Ovary Syndrome (PCOS): Clinical Features, Risk Factors, Biomarkers, Treatment, and Therapeutic Strategies

Fatima

Jeelani

Abass

et al. 2023

Toxicology and Human Health

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Follicular fluid and mural granulosa cells microRNA profiles vary in in vitro fertilization patients depending on their age and oocyte maturation stage

Cited by 59 publications

References 59 publications

Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

Changes of Ovarian microRNA Profile in Long-Living Ames Dwarf Mice during Aging

Polycystic Ovary Syndrome (PCOS): Clinical Features, Risk Factors, Biomarkers, Treatment, and Therapeutic Strategies

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