Follicular fat-soluble vitamins as markers of oocyte competency

Skowrońska, Patrycja; Kunicki, Michał; Pastuszek, Ewa; Konieczna, Lucyna; Bączek, Tomasz; Łukaszuk, Krzysztof

doi:10.1080/19396368.2020.1718244

Cited by 12 publications

(11 citation statements)

References 36 publications

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“…In the present study in women with unexplained infertility, we observed a significant positive correlation between serum and FF vitamin D levels. This finding is consistent with previous studies [ 6 , 14 – 16 ], showing that the serum and FF vitamin D levels significantly correlate. We did not observe significant differences between serum and FF vitamin D concentrations.…”

Section: Discussionsupporting

confidence: 94%

“…The present study analysed the correlation between serum and FF vitamin D levels with the percentage of embryo fragmentation. The results obtained in this study for correlation between FF vitamin D and the percentage of embryo fragmentation are similar to the results reported in the other studies [ 6 , 16 ]. This study shows a significant positive correlation between FF vitamin D and the percentage of embryo fragmentation.…”

Section: Discussionsupporting

confidence: 91%

“…During the study period, 382 women underwent IVF procedures, of whom 107 were with unexplained infertility (the prevalence of unexplained infertility was 28.01%). The application of study inclusion/exclusion criteria left 16 I. When compared, the vitamin D concentrations in serum and in FF showed no statistically significant differences (mean difference 4.6692, T 0.418, p = 0.684).…”

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confidence: 99%

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Follicular and serum levels of vitamin D in women with unexplained infertility and their relationship with in vitro fertilization outcome: an observational pilot study

Jeremic¹,

Miković²,

Soldatović

et al. 2021

Arch Med Sci

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IntroductionFollicular and serum vitamin D are considered potential markers of the oocyte and embryos' quality and predictors of IVF outcomes.Material and methodsThis retrospective cross-sectional study correlated vitamin D in sera and follicular fluid of women with unexplained infertility mutually and with IVF outcomes. ELISA was used for measuring Vitamin D.ResultsResults show only a positive correlation between follicular and serum levels of Vitamin D (Rho=0.615, p=0.025) and between follicular levels of Vitamin D with the percentage of embryo fragmentation (Rho=0.544; p=0.036).ConclusionsResults suggests that serum and follicular fluid vitamin D measurements could be complementary tools to the routine assessment of embryos.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 91%

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confidence: 99%

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Follicular and serum levels of vitamin D in women with unexplained infertility and their relationship with in vitro fertilization outcome: an observational pilot study

Jeremic¹,

Miković²,

Soldatović

et al. 2021

Arch Med Sci

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“…Retrieved mature oocytes were fertilized by intracytoplasmic sperm injection (ICSI). Embryos were cultured as previously described [ 35 ] and evaluated according to the 2011 Istanbul consensus criteria [ 36 ].…”

Section: Methodsmentioning

confidence: 99%

Compatibility of Distinct Label-Free Proteomic Workflows in Absolute Quantification of Proteins Linked to the Oocyte Quality in Human Follicular Fluid

Lewandowska

Fel

Thiel

et al. 2021

IJMS

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We present two separate label-free quantitative workflows based on different high-resolution mass spectrometers and LC setups, which are termed after the utilized instrument: Quad-Orbitrap (nano-LC) and Triple Quad-TOF (micro-LC) and their directed adaptation toward the analysis of human follicular fluid proteome. We identified about 1000 proteins in each distinct workflow using various sample preparation methods. With assistance of the Total Protein Approach, we were able to obtain absolute protein concentrations for each workflow. In a pilot study of twenty samples linked to diverse oocyte quality status from four donors, 455 and 215 proteins were quantified by the Quad-Orbitrap and Triple Quad-TOF workflows, respectively. The concentration values obtained from both workflows correlated to a significant degree. We found reasonable agreement of both workflows in protein fold changes between tested groups, resulting in unified lists of 20 and 22 proteins linked to oocyte maturity and blastocyst development, respectively. The Quad-Orbitrap workflow was best suited for an in-depth analysis without the need of extensive fractionation, especially of low abundant proteome, whereas the Triple Quad-TOF workflow allowed a more robust approach with a greater potential to increase in effectiveness with the growing number of analyzed samples after the initial effort of building a comprehensive spectral library.

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“…[7][8][9] Studies have shown that the vitamin A signaling pathway has potential effects on follicular development, oocyte maturation, ovarian steroidogenesis, and corpus luteum formation [10][11][12]. Also, studies showed that vitamin A level in follicular uid had an association with the quality of human oocytes, fertilization potential and embryo development [13]. However, Vitamin A de ciency (VAD) is common in women of reproductive age and even more serious in pregnant women.…”

Section: Introductionmentioning

confidence: 99%

Association between Serum Vitamin A Levels and Premature Ovarian Insufficiency Risk

Song¹,

Chen²,

Xu³

et al. 2021

Preprint

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Purpose: The aim of the study was to explore the association between serum vitamin A levels and premature ovarian insufficiency (POI). Methods: In this cross-sectional survey, women with POI (n = 47) and normo-ovulatory controls (n = 67) were enrolled from December 2016 to May 2018 in Zhejiang, China. The serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), anti-Müllerian hormone (AMH), vitamin A, and total cholesterol (TC) were measured for each participant. The association of vitamin A levels with POI risk was assessed using binary logistic regression analysis. Results: Serum vitamin A levels seems slightly higher in the POI group than in the control group (728.00 ± 176.00 vs 503.93 ± 145.64 µg/L, p = 0.133). But after adjust with serum lipid level, serum vitamin A/TC ratio was significantly lower in the POI group than in the control group (143.14 ± 35.86 vs 157.56 ± 35.21 µg/mmol, p = 0.035). Further, serum vitamin A/TC ratio was significantly and negatively associated with POI risk [unadjusted odds ratio (OR) = 0.988, 95% confidence interval (CI): 0.977–0.999, p = 0.039]. The same trend was found after adjusting for confounding factors (age, BMI, annual household income, and education) (OR = 0.986, 95% CI: 0.972–0.999, p = 0.040). Conclusion: Serum vitamin A/TC ratio was negatively associated with POI risk, indicating that vitamin A deficiency may be a risk factor for POI development. Serum vitamin A/TC ratio may serve as a predictive factor for POI incidence. Thus, vitamin A may serve a protective role in ovarian tissue.

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Follicular fat-soluble vitamins as markers of oocyte competency

Cited by 12 publications

References 36 publications

Follicular and serum levels of vitamin D in women with unexplained infertility and their relationship with in vitro fertilization outcome: an observational pilot study

Follicular and serum levels of vitamin D in women with unexplained infertility and their relationship with in vitro fertilization outcome: an observational pilot study

Compatibility of Distinct Label-Free Proteomic Workflows in Absolute Quantification of Proteins Linked to the Oocyte Quality in Human Follicular Fluid

Association between Serum Vitamin A Levels and Premature Ovarian Insufficiency Risk

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