Follicular Dendritic Cell Tumor of the Liver: A Clinicopathologic and Epstein-Barr Virus Study of Two Cases

Abstract

-12). In most of these cases, the sites affected were the lymph nodes. Extranodal cases were uncommon. Approximately 12% of FDC tumors were demonstrated to contain the EpsteinBarr virus (EBV) sequence (7). Therefore, EBV may play a role in the pathogenesis of the FDC tumor. In this report, we describe two FDC tumors of the liver with unique clinical features and a unique EBV subtype. MATERIALS AND METHODSTwo cases of hepatic FDC tumor were retrieved from the surgical pathology files of Chang Gung Memorial Hospital from the period 1996 to 1999. Formalin-fixed and paraffin-embedded tissues were used for histopathologic, immunohistochemical, and in situ hybridization studies. The immunohistochemical studies were performed using the avidin-biotin-peroxidase complex method. A panel of antibodies was used, and the antibodies are listed in Table 1. A fresh skin biopsy specimen (Case 1) was also subjected to direct immunofluorescence studies for IgG, IgM, IgA, C 3 , and C 4 . EBV-encoded nuclear RNA (EBER) in situ hybridization studies were performed with a DAKO fluoresceinconjugated EBV (EBER) PNA probe (complementary to two nuclear EBER RNAs encoded by EBV; DAKO A/S, Glostrup, Denmark) on 5-m-thick, deparaffinized, and proteinase K-pretreated tissues for 1.5 hour at 55°C. After washing, the reaction was detected by the DAKO PNA ISH detection Kit (DAKO A/S). Latent Membrane Protein-1 Gene StudyFresh tumor tissue obtained from Case 2 was frozen at Ϫ80°C until analyzed. High-molecular weight DNA was purified by phenol/chloroform ex-

Follicular Dendritic Cell Sarcoma of Parapharyngeal Space: A Case Report and Review of the Literature

“…It is also positive for clusterin, vimentin and fascin. EBER signals were demonstrated in some FDCS [15][16][17]. Follicular dendritic cell sarcoma has indolent behavior.…”

Inflammatory pseudotumor-like follicular dendritic cell tumor of the liver with expression of estrogen receptor suggests a pathogenic mechanism: a case report and review of the literature

“…LMP-1 gene is considered an EBV oncogene and is capable to transform rodent fibroblasts in vitro. EBV genome with point mutations or 30-bp deletion in exon 3 of LMP-1 gene is prevalent in Asia and was identified in one of two hepatic IPT-FDCT reported by Chen et al [12]. No EBV was identified in 24 cases of extranodal FDCS reviewed by Shia [16].…”

“…EBV was identified by LMP-1 or EBER only in the neoplastic spindle cells, but not in the lymphocytes or plasma cells, suggesting a pathogenic association of EBV. Clonal EBV genome was identified in hepatic IPT-FDCT [6,12], which suggests that EBV infection was an early event in the transformation of the tumor precursor cells. However, multiple genetic mutations are required for malignant transformation, which are largely unknown for IPT-FDCT.…”

“…Only one patient was reported dead of intra-hepatic and peritoneal dissemination 95 months after surgery. A review of the immunohistochemical reactivity of 15 cases [5,6,[9][10][11][12][13][14][15] revealed that 10/10 (100%) cases were positive for CD21, 7/9 (78%) positive for CD35, and five cases were either strongly positive or partial weak positive for a CD21/CD35 cocktail. Expression for other follicular dendritic cell markers was also observed, including 8/ 8 (100%) for CNA.42, 3/3 (100%) for Ki-M4P, and 2/2 (100%) for R4/23.…”

Hepatic inflammatory pseudotumour-like follicular dendritic cell tumor: A case report

“…Second, IPT-like FDC tumors always exhibit a marked female predominance (10). Third, IPT-like FDC tumors are strongly associated with the presence of EBV (11,12), although with a lower positivity rate compared with conventional FDC sarcomas (13). On pathological examination, IPT-like FDC tumors cells have a more prominent inflammatory component.…”