Follicle-stimulating hormone isoforms and plasma concentrations of estradiol and inhibin A in dairy cows with ovulatory and non-ovulatory follicles during the first postpartum follicle wave

Abstract

Following parturition, all cows display a wave of ovarian follicular growth, but a large proportion fail to generate a preovulatory rise in estradiol, and hence fail to ovulate. Follicle stimulating hormone (FSH) exists as multiple isoforms in the circulation depending on the type and extent of glycosylation, and this has pronounced effects on its biological properties. This 5 study examined differences in plasma FSH, estradiol, and inhibin A concentrations, and the distribution of FSH isoforms in cows with ovulatory or atretic dominant follicles during the first postpartum follicle wave. Plasma FSH isoform distribution was examined in both groups during the period of final development of the dominant follicle by liquid phase isoelectric focusing.Cows with an ovulatory follicle had higher circulating estradiol and inhibin A concentrations, 10 and lower plasma FSH concentrations. The distribution of FSH isoforms displayed a marked shift toward the less acidic isoforms in cows with ovulatory follicles. A higher proportion of the FSH isoforms had a pI >5.0 in cows with ovulatory follicles compared to those with atretic follicles. In addition, cows with ovulatory follicles had greater dry matter intake, superior energy balance, elevated circulating concentrations of insulin and insulin-like growth factor-I, 15 and lower plasma nonesterified fatty acids. The shift in FSH isoforms toward a greater abundance of the less acidic isoforms appears to be a key component in determining the capability for producing a preovulatory rise in estradiol, and this shift in FSH isoforms was associated with more favorable bioenergetic and metabolic status.

The impact of bisphenol S on bovine granulosa and theca cells

“…Treatment of the granulosa cells with BPS was performed in the presence or absence of 0.33 ng/mL FSH (oFSH‐19‐SIAFP; National Hormone & Peptide Program, Torrance, CA). This concentration of FSH was chosen to approximate the plasma levels of FSH during the final 6 days of follicle growth (Butler, Pelton, Knight, & Butler, ). Treatment of the theca cells with BPS was performed in the presence or absence of 100 pg/ml LH (oLH‐26; National Hormone & Peptide Program, Torrance, CA).…”

Molecular regulation of follicle-stimulating hormone synthesis, secretion and action

“…After ovarian failure that normally occurs as a function of aging, more acidic FSH becomes dominant and significantly detectable in older premenopausal women (Thomas, et al 2009). A change in FSH isoform abundance, toward less acidic molecules was also observed in cows with ovulatory follicles compared to those with atretic follicles, and this shift in FSH isoforms is suggested to decide the capability for producing a preovulatory estradiol rise (Butler, et al 2008). Factors like age, sex and reproductive state can also influence the formation of the type of the FSH isoforms in sheep pituitary (Moore, et al 2000).…”

Estudo de associação genômica de prenhez pós-parto em vacas de corte

“…Reproductive efficiency is a major factor affecting livestock production. Ovarian activity during postpartum period depends on follicle stimulating hormone (FSH), luteinizing hormone (LH), insulin like growth factor (IGF-1) (BUTLER et al, 2008;THIENGTHAM;PARKINSON;HOLMES, 2008), leptin (LEP) and their receptors (LIEFERS et al, 2003;LIEFERS et al, 2005;PASSOS et al, 2007). At the end of pregnancy, hypothalamic-pituitary axis responds to a negative feedback effect from placental and ovarian steroids by suppressing FSH release and depleting LH stores WALTON, 2000a).…”