Follicle-stimulating hormone-dependent phosphorylation of vimentin in cultures of rat Sertoli cells.

Spruill, W A; Steiner, Alton L.; Tres, Laura L.; Kierszenbaum, Abraham L.

doi:10.1073/pnas.80.4.993

Cited by 67 publications

(36 citation statements)

References 29 publications

(31 reference statements)

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“…Activation of cAMP-dependent protein kinases results in the phosphorylation of specific protein substrates (3), which in turn modulate many of the subsequent physiological cell responses (4 (5,6). FSH-dependent phosphorylation of vimentin is preceded by a surge of both intracellular cAMP and protein-bound cAMP levels (6)(7)(8), which are maximized by using a phosphodiesterase inhibitor. Proteinbound cAMP levels reflect the binding of cAMP to regulatory subunits of cAMP-dependent protein kinases as determined by using a photoaffinity analog (8 (27).…”

Section: Methodsmentioning

confidence: 99%

“…FSH action on rat Sertoli cells in primary culture is mediated in part by the activation of cAMP-dependent protein kinases (1,2). Activation of cAMP-dependent protein kinases results in the phosphorylation of specific protein substrates (3), which in turn modulate many of the subsequent physiological cell responses (4 (5,6). FSH-dependent phosphorylation of vimentin is preceded by a surge of both intracellular cAMP and protein-bound cAMP levels (6)(7)(8), which are maximized by using a phosphodiesterase inhibitor.…”

Section: Methodsmentioning

confidence: 99%

“…Immunocytochemical experiments were carried out as described (6,7). Controls for specificity included (i) omission of primary antiserum, (ii) absorption of antiserum with an extract of intermediate filament proteins (6), and (iii) immunoprecipitation of [35S]_ methionine-labeled vimentin (6).…”

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confidence: 99%

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Rat Sertoli cells acquire a beta-adrenergic response during primary culture.

Kierszenbaum¹,

Spruill²,

White³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Rat Sertoli cells acquire a beta-adrenergic response during primary culture.

Kierszenbaum¹,

Spruill²,

White³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

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“…In this experiment, when 15-day testes were cultured, [17]. This may be the case in the present study on fetal Sertoli cells.…”

Section: Discussionmentioning

confidence: 88%

Effects of Follicle-Stimulating Hormone on Intermediate Filaments and Cell Division of Sertoli Cells of Fetal Rat Testis in Culture.

Sasaki

Yamamoto

Arishima

et al. 1998

The Journal of Veterinary Medical Science

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ABSTRACT. The present study was aimed to examine the effects of follicle-stimulating hormone (FSH) on cell division of Sertoli cells from rat fetal testes and on the kinetics of 2 kinds of intermediate filaments, cytokeratin and vimentin, which comprise the cytoskeleton of Sertoli cells. Testes from rat fetuses of different ages (from day 15 to day 17 of gestation ) were cultured for 48 hr, with or without added FSH. for the presence of sperm in the vaginal smear. The day on which sperm was detected was counted as day 0 of gestation. Fetuses of 15-17 days of age were used for culture of their testes. These fetuses were removed from maternal uteri under aseptic conditions and transferred to sterile petri dishes containing Hanks' balanced salt solution (HBSS) with penicillin (100 units/ml) and streptomycin (100 µg/ml). Their testes were removed and rinsed with HBSS. Each testis was placed on the surface of a Millipore filter in the medium (Medium RPMI 1640; Gibco-BRL, U.S.A.) containing 0.2% sodium bicarbonate. Three series of culture were prepared. One was added with no FSH as control (group C). The second was added with 0.5 µg/ml FSH (Sigma, 1 mg equivalent to 1 unit shown by Steelman and Pohley [18]) as group FSH 0.5. The third was added with 5 µg/ml FSH as group FSH 5. The Millipore filter rested on a stainless-steel grid in a culture dish (Nunc, Roskilde, Denmark). All the cultures were incubated at 37˚C in a humidified atmosphere of 5% CO 2 and 95% O 2 for 48 hr. All of the above procedures were performed under sterile conditions.After incubation, the cultures were immediately frozen in liquid nitrogen. Frozen sections (8 µm) were air-dried for a few min, fixed in acetone at 20˚C for 10 min and allowed to be air-dried. The indirect immunofluorescence with amplification by the biotin-streptavidin system was used. Sections were incubated with monoclonal antibody to cytokeratin (clone Lu5, Boeringer Mannheim GmbH, Germany) or vimentin (clone V 9, Biodesign International, U.S.A.) and visualized with rhodamine X isothiocyanate (XRITC) or fluorescein isothiocyanate (FITC), respectively.To measure the proliferative activity of Sertoli cells in cultured testes, in another series of experiments, 10 µM 5-bromo-2'-deoxyuridine (BrdU) was added to the culture medium after 24 hr-incubation and the incubation was continued for 2 hr. Then, each explant was transferred Many studies have been made on differentiation and development of rat testes during the fetal period. In the formation of seminiferous tubule in the rat [20] and mouse [3], several mesenchyme-derived cells initially surround primordial germ cells to create a tube-like shape in the gonadal primordium. These mesenchyme-derived cells are considered to differentiate into Sertoli cells thereafter [7,8].It has been reported that intermediate filaments, cytokeratin and vimentin, are simultaneously found in Sertoli cells during the fetal period [15]. Cytokeratin disappears on 14 days after birth. Rat Sertoli cells show active cell division and proliferation during t...

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“…Application of fl-adrenergic agonists to cultured cilial epithelium results in a profound rearrangement of cytoskeletal proteins and the appearance of a stellate morphology in these cells [15]. Application of follicle-stimulating hormone to cultured Sertoli cells results in similar morphological and cytoskeletal changes [16]. Electrical stimulation of cochlear hair cells produces changes in their length [17].…”

Section: Discussionmentioning

confidence: 99%

Light‐ and nucleotide‐dependent increase in apparent viscosity in a suspension of retinal disks

Caretta

Stein

1987

FEBS Letters

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Light triggers the cyclic nucleotide cascade in photoreceptor disk membranes. We report here that lightinduced changes in the apparent viscosity of disk membrane suspensions can also be observed using either native disk membranes or washed membranes reconstituted with G protein and PDE. The viscosity changes are light-and GTP-dependent and require the presence of G protein and PDE. The magnitude of the viscosity change increases with increasing membrane concentration. Under the same conditions in which light elicits a change in viscosity, we observe a large increase in light scattering by the disk membrane suspension.

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Follicle-stimulating hormone-dependent phosphorylation of vimentin in cultures of rat Sertoli cells.

Abstract: Endogenous protein phosphorylation was investigated in cultured rat Sertoli cells after treatment with folliclestimulating hormone (FSH) 3',, N-morpholinepropanesulfonic acid (Mops), EGTA, ATP, and cAMP were from Sigma.

Cited by 67 publications

References 29 publications

Rat Sertoli cells acquire a beta-adrenergic response during primary culture.

Rat Sertoli cells acquire a beta-adrenergic response during primary culture.

Effects of Follicle-Stimulating Hormone on Intermediate Filaments and Cell Division of Sertoli Cells of Fetal Rat Testis in Culture.

Light‐ and nucleotide‐dependent increase in apparent viscosity in a suspension of retinal disks

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