Follicle priming by FSH and pre-maturation culture to improve oocyte quality in vivo and in vitro

Sakaguchi, Kenichiro; Nagano, Masashi

doi:10.1016/j.theriogenology.2020.01.023

Cited by 10 publications

(5 citation statements)

References 80 publications

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“…The oocyte resumes meiotic division to the metaphase II (MII) stage in vivo after the LH hormone surge and in vitro when it removes from antral ovarian follicle and cultured in favorable medium within 5.0% CO2 incubator (Grabarek et al, 2004;Mohammed 2006). Development of in vivo matured oocytes to embryos is higher than in vitro matured ones (Margalit et al, 2019;Sakaguchi and Nagano, 2020). This might be attributed to insufficient nucleus and/or cytoplasmic maturity (Blondin et al, 1997).…”

Section: Factors Affecting Oocyte Maturationmentioning

confidence: 98%

Changes in Nucleus and Cytoplast During Oocyte Maturation: Involvement in Embryo Production

Zeidi¹,

Masruri²,

Mufarji³

et al. 2022

AAVS

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Embryo production steps either in vivo or in vitro include oocyte meiotic maturation, fertilization and culture processes. Oocyte maturation is the first step for in vivo or in vitro embryo production. Oocyte maturation appears to be a simple step, but it is a very complex process. Maturation of oocytes include several changes at the cytoplasmic and nuclear levels that makes the oocytes able to undergo fertilization, pre-implantation and post-implantation embryo development. Oocyte meiotic maturation in vivo starts after LH hormone surge whereas it starts in vitro upon release of oocyte from the ovarian follicle. Rodent oocyte maturation lasts 15-17hr. whereas that of ruminant oocyte lasts 24hr. Several factors affect on oocytes maturation either in vitro or in vivo and therefore affect development of the resulting embryos. Because of the significance of oocyte maturation stage on further embryonic development, this review aims to discuss the knowledge of oocyte maturation and its effect on further development of embryos in vivo and in vitro.

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Section: Factors Affecting Oocyte Maturationmentioning

confidence: 98%

Changes in Nucleus and Cytoplast During Oocyte Maturation: Involvement in Embryo Production

Zeidi¹,

Masruri²,

Mufarji³

et al. 2022

AAVS

View full text Add to dashboard Cite

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“…Currently, oocytes undergoing IVM cannot achieve complete cytoplasmic maturation, and the basal medium needs to be supplemented with numerous known nutrients to promote oocyte maturation and development during IVM. FSH is one of the critical components of IVM media [18]. In goats [111], cattle [18], and mice [112], the addition of FSH to the oocyte maturation system in vitro increases the oocyte maturation rate by promoting cumulus expansion and influencing the cAMP level.…”

Section: In Vitro Technology Promotes the Study Of Oocyte Maturationmentioning

confidence: 99%

“…FSH is one of the critical components of IVM media [18]. In goats [111], cattle [18], and mice [112], the addition of FSH to the oocyte maturation system in vitro increases the oocyte maturation rate by promoting cumulus expansion and influencing the cAMP level.…”

Section: In Vitro Technology Promotes the Study Of Oocyte Maturationmentioning

confidence: 99%

“…In vivo, oocyte maturation is regulated by numerous factors (e.g., follicle-stimulating hormone (FSH), luteinizing hormone (LH)) and the interaction between oocytes and granulosa cells [15][16][17]. In vitro, various medium formulations have been evaluated to create conditions more similar to those in vivo during oocyte maturation [18,19]. In the past few decades, improving oocyte maturation rates and oocyte quality in vitro has been the focus of many medical scientists.…”

Section: Introductionmentioning

confidence: 99%

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Advances in Oocyte Maturation In Vivo and In Vitro in Mammals

Jiang

Pan

et al. 2023

IJMS

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The quality and maturation of an oocyte not only play decisive roles in fertilization and embryo success, but also have long-term impacts on the later growth and development of the fetus. Female fertility declines with age, reflecting a decline in oocyte quantity. However, the meiosis of oocytes involves a complex and orderly regulatory process whose mechanisms have not yet been fully elucidated. This review therefore mainly focuses on the regulation mechanism of oocyte maturation, including folliculogenesis, oogenesis, and the interactions between granulosa cells and oocytes, plus in vitro technology and nuclear/cytoplasm maturation in oocytes. Additionally, we have reviewed advances made in the single-cell mRNA sequencing technology related to oocyte maturation in order to improve our understanding of the mechanism of oocyte maturation and to provide a theoretical basis for subsequent research into oocyte maturation.

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“…FSH is one of the essential components of IVM media [8,14]. Numerous reports have functionally demonstrated the importance of FSH for the acquisition of oocyte developmental competence [14,[23][24][25][26]. Since FSH regulates (directly and indirectly) several metabolic processes in oocytes and cumulus cells during IVM [8,14], FSH withdrawal may reveal insights into cumulus-oocyte complexes (COCs) metabolic activities during IVM.…”

Section: Introductionmentioning

confidence: 99%

Follicle-stimulating hormone mediates the consumption of serum-derived glycogen by bovine cumulus-oocyte complexes during in vitro maturation

et al. 2021

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Background and Aim: Oocyte in vitro maturation (IVM) is an appealing approach for several assisted reproductive technologies and dissecting oocyte maturation. Nonetheless, IVM leads to lower developmental competence and usually relies on undefined, serum-containing media. Therefore, biochemical profiling aimed to explore fluctuations in IVM media content during the acquisition of oocyte developmental competence. Materials and Methods: Bovine cumulus-oocyte complexes (COCs) underwent IVM in TCM199 medium with Earle's salts, supplemented with 2.0 mM L-glutamine, 10% fetal bovine serum, antibiotics, and 0.05 IU/mL porcine follicle-stimulating hormone (FSH+) or vehicle control (CTL) medium for 22 h. Results: FSH withdrawal (CTL) diminished several processes associated with the acquisition of oocyte developmental competence, such as reduced cumulus cell expansion, diminished estradiol synthesis (FSH+: 116.0±0.0 pg/mL vs. CTL: 97.6±18.0 pg/mL), and lower oocyte nuclear maturation rate (FSH+: 96.47% vs. CTL: 88.76%). Fresh media formulations (i.e., TCM199 with FSH or vehicle) were indistinguishable under biochemical profiling threshold conditions. Biochemical profiling showed similar total protein and lipid concentrations between groups. Further, total sugar concentrations diminished from fresh media to their post-IVM counterparts, albeit in an FSH-independent manner. Glycogen concentrations remained unaltered after IVM within CTL media, albeit were substantially lower after IVM under FSH+ conditions. Conclusion: FSH mediates the consumption of serum-derived glycogen by bovine COCs during IVM and implies that serum-free media should contain increased glucose concentrations to facilitate the acquisition of oocyte developmental competence.

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Follicle priming by FSH and pre-maturation culture to improve oocyte quality in vivo and in vitro

Abstract: In vivo matured oocytes have higher competence than in vitro matured oocytes.• A single FSH injection prior to OPU can improve oocyte competence.• In vitro pre-maturation enables to improve oocyte competence without FSH priming.

Cited by 10 publications

References 80 publications

Changes in Nucleus and Cytoplast During Oocyte Maturation: Involvement in Embryo Production

Changes in Nucleus and Cytoplast During Oocyte Maturation: Involvement in Embryo Production

Advances in Oocyte Maturation In Vivo and In Vitro in Mammals

Follicle-stimulating hormone mediates the consumption of serum-derived glycogen by bovine cumulus-oocyte complexes during in vitro maturation

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