Follicle microenvironment-associated alterations in gene expression in the mouse oocyte and its polar body

Jiao, Ze Xu; Woodruff, Teresa K.

doi:10.1016/j.fertnstert.2012.12.009

Cited by 20 publications

(16 citation statements)

References 42 publications

(47 reference statements)

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“…Their results demonstrated that detection and quantification of mRNA in human PBs is possible and that the human PB mRNA transcriptome reflects that of its sibling MII oocyte [9]. This work and our data demonstrated that PB 1 mRNA is being considered as a proxy for the oocyte [29].…”

Section: Discussionsupporting

confidence: 65%

Age-related increase in aneuploidy and alteration of gene expression in mouse first polar bodies

Jiao

Woodruff

2014

J Assist Reprod Genet

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Purpose To confirm that aneuploidy candidate genes are detectable in the first polar body (PB 1 ) of MII oocytes and to investigate the age-dependent molecular changes in PB 1 . Methods Aged (12-to 15-mo-old) and young (2-mo-old) mice were administered pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotrophin (hCG). MII oocytes were obtained and the first PB was removed. mRNA from each PB and its sibling oocyte was reverse transcribed. Real-time PCR was performed to quantify the expression of six genes (BUB1, CDC20, Filia, MCAK, SGOL1, SMC1A) in single PB. Results We first demonstrated that detection and quantification of transcripts associated with aneuploidy in single mouse oocyte and sibling PB 1 is possible and the relative abundance of mRNA transcripts in a single PB faithfully reflects the relative abundance of that transcript in its sibling oocyte. We further found that transcript levels were significantly lower in aged PBs compared with young PBs (P<0.05). Conclusions Our results suggest that the detection and analysis of polar body mRNA may provide insight in age-related aneuploidy in oocyte. This analysis is a novel concept to investigate the genesis of chromosome abnormality and could potentially assist in the characterization of mechanisms underlying key molecular origin of female meiotic aneuploidy, which would be of great scientific and clinical value.

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Section: Discussionsupporting

confidence: 65%

Age-related increase in aneuploidy and alteration of gene expression in mouse first polar bodies

Jiao

Woodruff

2014

J Assist Reprod Genet

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“…Our prior work quantified 6 maternal-effect mRNA transcripts in PB 1 from young and aged mouse MII oocytes and demonstrated that there is a significant difference in the transcript levels of oocyte-specific genes in aged vs. young PB that correlates with age-related decreases in oocyte competence (17). This study and our previous data led us to propose that a PB biopsy to measure maternal-effect mRNA abundance may serve as biomarkers of embryo competence (39). Future studies are needed to evaluate dynamic changes in mRNA turnover in PB 2 , as well as to identify a cohort of transcripts that are reliably detected in PB 2 , whose expression levels are predictive of the developmental competence of a given zygote and its resulting embryo.…”

Section: Discussionmentioning

confidence: 70%

Detection and quantification of maternal-effect gene transcripts in mouse second polar bodies: potential markers of embryo developmental competence

Jiao¹,

Woodruff²

2013

Fertility and Sterility

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Objective To test the hypothesis that quantification of mRNAs originating the second polar body (PB2) provides a non-invasive tool for assessing embryo quality. Design Prospective study Setting Hospital-based academic research laboratory Animals CD1 female mice Intervention(s) MII oocytes obtained from 7- to 8-week-old mice after pregnant mare’s serum gonadotrophin (PMSG) and human chorionic gonadotrophin (hCG) priming. After in vitro fertilization, the PB2 was biopsied from zygotes followed by reverse transcription. Real-time PCR was performed to quantify gene expression levels in single PB2. The sibling zygotes were continuously cultured to blastocyst stage. Main Outcome Measure(s) Embryo developmental competence and six maternal-effect gene (Dnmt1, Mater, Nobox, Npm2, Tcl1 and Zar1) transcripts in the PB2 Results PB2 mRNA was detected in all candidate genes. Transcripts that were present in greater abundance in the zygote were more likely to be detected in qPCR replicates from single PB2. 4 candidate genes (Dnmt1, Nobox, Npm2 and Tcl1) expression levels in PB2 between two groups (2-cell embryo vs. blastocyts) approached statistical significance. Conclusion PB2 may contain a representative transcript profile to that of the zygote after fertilization. Differences in gene expression in PB2 may be potential biomarkers of embryo quality.

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“…Nevertheless, in this study the developmental competence of the oocytes was compromised by the high incidence of abnormal spindle formation and chromosome alignment. Variations in rigidity of the hydrogel also can affect the gene-expression pattern, as shown by Jiao and Woodruff (2013). Oocyte-specific gene-transcript levels in cultured oocytes and polar bodies were decreased in oocytes cultured in 1.5% alginate compared with 0.25% (Jiao and Woodruff 2013).…”

Section: Application Of Alginate Hydrogels To In Vitro Follicle Culturementioning

confidence: 99%

Three-dimensional systems for in vitro follicular culture: overview of alginate-based matrices

Brito

Lima

et al. 2014

Reprod. Fertil. Dev.

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The in vitro culture of ovarian follicles has provided critical insight into the biology of the follicle and its enclosed oocyte and the physical interaction and communication between the theca and granulosa cells and the oocyte that is necessary to produce meiotically competent oocytes. Various two-dimensional (2D) and three-dimensional (3D) culture systems have been developed to evaluate the effect of growth factors, hormones, extracellular matrix components and culture conditions on follicle development and oocyte growth and maturation. Among these culture systems, 3D systems make it possible to maintain follicle structure and support communication between the various cell compartments within the follicle. In this review article, we will discuss the three main approaches to ovarian follicle culture: 2D attachment systems, 3D floating systems and 3D encapsulated systems. We will specifically emphasise the development of and advances in alginate-based encapsulated systems for in vitro follicle culture.

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Follicle microenvironment-associated alterations in gene expression in the mouse oocyte and its polar body

Cited by 20 publications

References 42 publications

Age-related increase in aneuploidy and alteration of gene expression in mouse first polar bodies

Age-related increase in aneuploidy and alteration of gene expression in mouse first polar bodies

Detection and quantification of maternal-effect gene transcripts in mouse second polar bodies: potential markers of embryo developmental competence

Three-dimensional systems for in vitro follicular culture: overview of alginate-based matrices

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