Folic Acid-Grafted Chitosan-Alginate Nanocapsules as Effective Targeted Nanocarriers for Delivery of Turmeric Oil for Breast Cancer Therapy

Abstract

Folate receptors (FRs) highly expressed in breast cancers can be used as a recognized marker for preventing off-target delivery of chemotherapeutics. In this study, folic acid (FA)-grafted chitosan-alginate nanocapsules (CS-Alg-NCs) loaded with turmeric oil (TO) were developed for breast cancer targeting. CS was successfully conjugated with FA via an amide bond with a degree of substitution at 12.86%. The TO-loaded FA-grafted CS-Alg-NCs (TO-FA-CS-Alg-NCs) optimized by Box-Behnken design using response surface methodology had satisfactory characteristics with homogenous particle size (189 nm) and sufficient encapsulation efficiency and loading capacity (35.9% and 1.82%, respectively). In vitro release study of the optimized TO-FA-CS-Alg-NCs showed a sustained TO release following the Korsmeyer-Peppas model with a Fickian diffusion mechanism at pH 5.5 and 7.4. The TO-FA-CS-Alg-NCs showed lower IC50 than ungrafted TO-CS-Alg-NCs and unencapsulated TO against MDA-MB-231 and MCF-7 breast cancer cells, suggesting that FA-CS-Alg-NCs can improve anticancer activity of TO through its active targeting to the high FRs expressing breast cancers.

Phytoconstituent analysis, anti-inflammatory, antimicrobial and anticancer effects of nano encapsulated Convolvulus arvensis L. extracts

“…The CS and Alg polymers were attributed to having low toxicity and hemocompatibility [ 57 ]. San et al [ 58 ], suggested that both the unbound TO and its combination with TO-FA-Cs/Alg-NPs demonstrated minimal hemolytic activity (5%). However, when exposed to the maximum TO concentration (7.5–10 mg/ml), this behavior changed, suggesting that they might be suitable for human use.…”

Alginate-Chitosan Biodegradable and Biocompatible Based Hydrogel for Breast Cancer Immunotherapy and Diagnosis: A Comprehensive Review

“…This finding indicates that the hydrogel improves the anticancer effectiveness in the case of breast cancers with high folate receptor (FR) expression. 190 Chitosan, a β (1−4) glycan resulting from chitin Ndeacetylation has accumulated attention in drug delivery due to its capacity to regulate immunological reactions and biological compatibility. It has received approval from the FDA for applications in the food and pharmaceutical industries.…”

“…With a comprising study, it was found that TO-FA-CS-Alg-NCs have lower cell viability activity against the breast cancer cell lines. This finding indicates that the hydrogel improves the anticancer effectiveness in the case of breast cancers with high folate receptor (FR) expression …”

“…This finding indicates that the hydrogel improves the anticancer effectiveness in the case of breast cancers with high folate receptor (FR) expression. 190 …”

Cytotoxicity Enhancement of α-Mangostin with Folate-Conjugated Chitosan Nanoparticles in MCF-7 Breast Cancer Cells

“…Overall, the conjugation of folate to CSNPs can significantly impact their size and biological properties, and these factors should be carefully considered in the design and development of targeted drug-delivery systems [66,68,69].…”