Folic Acid Functionalized PFBT Fluorescent Polymer Dots for Tumor Imaging

Cao, Fengwen; Xiong, Liqin

doi:10.1002/cjoc.201500780

Cited by 22 publications

(18 citation statements)

References 21 publications

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“…The FA-MEH-PPV-COOH, MEH-PPV-COOH, FA-MEH-PPV-NH 2 and MEH-PPV-NH 2 polymer dots were prepared by the coprecipitation according to our previous study, [39][40][41][42][43] with some modications. In a typical procedure, a solution of 2 mL of THF containing 250 mg MEH-PPV and 250 mg PS-PEG-COOH or 250 mg MMA-NH 2 and 5 mg NIR775 with/without 50 mg PE-FA was mixed in stock.…”

Section: Preparation Of Polymer Dotsmentioning

confidence: 99%

“…And NIR emission can be realized based on the uorescence resonance energy transfer (FRET) effect. 30 In this study, NIR775-doped poly [2-methoxy-5-(2-ethylhexyloxy)-1,4-phenylenevinylene] (MEH-PPV) polymer dots 39 were used as NIR uorescence imaging probes for in vitro and in vivo cell tracking macrophage Ana-1 cells. In addition, by combining probe-based confocal laser endomicroscopy (pCLE), real-time imaging macrophage cell in deep organs and lymphatics were obtained.…”

Section: Introductionmentioning

confidence: 99%

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High resolution tracking of macrophage cells in deep organs and lymphatics using fluorescent polymer dots

et al. 2019

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In vivo cell tracking can provide information on cell migration and accumulation in the organs. Here, both folate and amino modified polymer dots were synthesized and screened for in vitro and in vivo tracking of macrophage Ana-1 cells. Flow cytometry analysis demonstrated that prepared polymer dots showed cellular uptake of approximately 98% within a short incubation time of 2 h, and these polymer dots maintained a cell labeling rate over 97% after 2 d. Moreover, a CCK-8 assay suggested that these polymer dots increased Ana-1 cell viabilities up to 110% at concentrations from 5 to 50 mg mL À1 . Furthermore, the in vivo real time imaging of labelled Ana-1 cells in the alveolus of lung and lymph nodes were clearly detected by probebased confocal laser endomicroscopy (pCLE). This study demonstrates a unique approach using polymer dots for real-time high resolution tracking of macrophage cells in deep organs and the lymphatic system.

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Section: Preparation Of Polymer Dotsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High resolution tracking of macrophage cells in deep organs and lymphatics using fluorescent polymer dots

et al. 2019

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“…[2][3][4][5][6][7][8][9][10] The new generation biomaterial is thought to be bioactive as well as biodegradable. [11][12][13][14][15][16][17][18] It is very meaningful to study cell-material interactions. [19][20][21] A lot of cues have been known to influence cell behaviors.…”

Section: Introductionmentioning

confidence: 99%

Left‐Right Symmetry or Asymmetry of Cells on Stripe‐Like Micropatterned Material Surfaces

Yao

Liu

et al. 2018

Chin. J. Chem.

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Material surfaces can induce cell responses such as contact guidance, yet little attention has been paid to further cell orientation. Herein, we report an interesting phenomenon of cell orientation beyond the classic contact guidance on a stripe‐like micropattern with cell‐adhesive arginine‐ glycine‐aspartate (RGD) peptides on a nonfouling background decorated by poly(ethylene glycol) (PEG). Such a micropattern with cell adhesion contrast led to significant contact guidance after cell seeding. What is more, the localized and elongated cells were found to be further orientated out of the adhesive stripes, and even an anticlockwise rotation was observed for rat mesenchymal stem cells (rMSCs). The left‐right asymmetry of rMSCs stood only in statistics, for we observed all cases including clockwise orientation, anticlockwise orientation or just keeping the orientation of previous contact guidance. We further found that human foreskin fibroblasts (HFFs) preferred a clockwise rotation, while human mesenchymal stem cells (hMSCs) and human umbilical vascular endothelial cells (HUVECs) exhibited no significant preference to either direction, which indicated that the left‐right symmetry or asymmetry was cell‐type dependent. The present report has partially confirmed the cell chirality and revealed its complexity, calling for further careful and comprehensive investigation of the challenging topic of cell chirality on material surfaces.

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“…For example, in the case of a nanosystem formed by gold nanoparticles and quantum dots, the final emitted fluorescence intensity of which could either be enhanced or quenched (turned “ON” or “OFF”) by altering the distance between the two components, thus realizing real‐time tracing at desired sites or situations . One representative example here is the fluorescent polymer dots poly(9,9‐dioctylfluorene‐2,7‐diyl‐ co ‐benzothiadiazole) (PFBT; Figure S1, Supporting Information), a class of macromolecules with various π‐extended bridges, have emerged as powerful contrast agent due to their high brightness, good quantum yield, excellent photostability, and good biocompatibility . Here, PFBT could be dispersed into aqueous solutions by conjugating itself onto the HGCNs though the AuS bond.…”

Section: Introductionmentioning

confidence: 99%

Hollow Au–Cu Nanocomposite for Real‐Time Tracing Photothermal/Antiangiogenic Therapy

Pang

Tan

Wang

et al. 2017

Adv Healthcare Materials

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High absorption in the near-infrared (NIR) region is essential for a photoabsorbing agents to realize efficient photothermal therapy (PTT) for cancer. Here, a novel hollow Au-Cu nanocomposite (HGCNs) is developed, which displays a significantly enhanced NIR surface plasmon resonance absorption and photothermal transduction efficiency. Besides, fluorescent polymer dots poly(9,9-dioctylfluorene-2,7-diyl-co-benzothiadiazole) (PFBT) and chemotherapeutic mammalian target of rapamycin (mTOR) inhibitor agent rapamycin (RAPA) are attached onto the HGCNs (RAPA/PFBT-HGCNs) for real-time NIR fluorescence tracing and combined PTT/antiangiogenesis therapy. In particular, due to the fluorescence resonance energy transfer effect, RAPA/PFBT-HGCNs can act as NIR-activatable on/off probe system for real-time tracing of tumor tissues. A standard in vitro cellular uptake study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, dual-staining study, and flow cytometry assay reveal that the RAPA/PFBT-HGCNs combined with NIR laser exhibit higher drug accumulation and cytotoxicity in both tumor cells and epithelial cells. Moreover, the margins of tumor and normal tissue can be accurately indicated by NIR-stimulated dequenched PFBT after 24 h intravenous administration. Further, tumor growth can be considerably hampered by the optimal formulation plus laser treatment with relatively lower side effects. Consequently, the work highlights the real-time tracing and enhanced PTT/antiangiogenesis therapy prospects of the established HGCNs with tremendous potential for treatment of cancer.

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Folic Acid Functionalized PFBT Fluorescent Polymer Dots for Tumor Imaging

Cited by 22 publications

References 21 publications

High resolution tracking of macrophage cells in deep organs and lymphatics using fluorescent polymer dots

High resolution tracking of macrophage cells in deep organs and lymphatics using fluorescent polymer dots

Left‐Right Symmetry or Asymmetry of Cells on Stripe‐Like Micropatterned Material Surfaces

Hollow Au–Cu Nanocomposite for Real‐Time Tracing Photothermal/Antiangiogenic Therapy

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