Folic Acid Determination in Food Samples Using Green Synthesized Copper Oxide Nanoparticles and Electro-Poly (Methyl Orange) Sensor

Abstract

Copper (II) oxide nanoparticles (CuONPs) were green synthesized using Ocimum basilicum leaves aqueous extract in which polyphenols act as reducing and stabilizing agents. The synthesized CuONPs were characterized using X-ray diffraction spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscopy, selected area electron diffraction, and Brunauer–Emmett–Teller (BET) surface area analysis. The analyses indicated the formation of crystalline rod-like monoclinic pure CuONPs with a mean grain size of 15 nm, a surface area of 396 m2 g−1, and a total pore volume of 0.71 cm3 g−1. A glassy carbon electrode (GCE) was modified using the synthesized CuONPs and electropolymerized poly(methyl orange) (PMO). The modified PMO/CuONPs/GCE electrode was electrochemically characterized and applied for the estimation of folic acid (FA) by cyclic voltammetry, chronoamperometry, linear sweep voltammetry, and differential pulse voltammetry techniques. The influence of pH (7), scan rate (50 mV/s), supporting electrolyte (0.1 M KCl) and FA concentration has been optimized. FA is precisely determined in the range from 0.01 to 1.5 µΜ with a low detection limit (0.002 µΜ), a low quantitation limit (0.068 µΜ), high reproducibility (RSD 0.37, 10 measurements), and high stability (98% activity after 50 days). FA in food samples was determined by the new sensor with high recoveries from 93 to 108.8%. Graphical Abstract

Electrochemical biosensing based on folic acid-triazine-grafted reduced graphene oxide: a highly selective breast cancer cell sensor

“…24 The slight red-shift of similar peaks for FA-RGOTrz approves the binding of FA on the RGOTrz surface. 25…”

“…24 The slight red-shi of similar peaks for FA-RGOTrz approves the binding of FA on the RGOTrz surface. 25 Aer characterizing the changes by EDX, XRD, FT-IR spectroscopy and UV-vis spectroscopy, they were characterized by CV and EIS, and the results are shown in Fig. 3.…”

Advances in Folic Acid Biosensors and Their Significance in Maternal, Perinatal, and Paediatric Preventive Medicine

“…The electrode exhibited an RSD of 3.52% and about 6.8% response loss over 4 weeks [90]. Lastly, a GCE modified with Cu(II)O nanoparticles and electropolymerised polymethyl orange realized a detection limit of 2 nM with linearity over 0.01 µM to 1.5 µM at pH 7 with an RSD of 0.37% and only 1.6% response loss over 50 days [91]. Like most electrochemical folate sensing electrodes, these electrodes sense folate vitamers irrespective of glutamate status.…”

Recent Trends and Application of Nanomaterial Based on Carbon Paste Electrodes: A Short Review

“…The reusability test of the MMIPs in the electrochemical folate detection exhibits good accuracy and can be used up to five times. Hasan et al 64) used copper oxide nanoparticles generated from osmium basilicum leaves to modify CPEs. High stability of 98% and recovery of 108.8% make copper oxide nanoparticles perform effectively in the electroanalysis of folic acid, with a detection limit of 2x10 -6 M and a linear range of 0.01 to 1.5 M. According to the results of the two studies, iron (III) acetylacetonate exhibited better electrochemical activity than copper oxide nanoparticles in detection of folate.…”