Folic acid conjugated and Ag-carrying organoclay nanoparticles and their response to L929 fibroblast and DLD-1 cancer cells

Abstract

This work presents the synthesis and characterisation of intercalated nanocomposites (NCs) from dispersed water solution blends of octadecyl amine-montmorillonite (ODA-MMT) (NC-0), folic acid (FA) conjugated ODA-MMT (NC-1) and Ag-MMT clay as a stable silver carrying agent (NC-2). The composition, chemical/physical and morphology of NCs with in situ intercalating nanostructures were investigated. Effect of organoclay, FA and Ag-MMT on L929 fibroblast (control), human colon carcinoma (DLD-1) cell lines, and the cytotoxicity, apoptosis and necrosis degree were estimated via WST-1/hemocytometric, double staining (as a ribonuclease A enzyme based method) and fluorescence microscopy methods in a dose-dependent manner. The mentioned cell lines integrated with NCs resulted in remarkable change in both morphology and nuclei of DLD-1 and fibroblast cells by apoptosis analysis. The number of necrotic cells were remarkably increased, as the toxic effects of nanocomposite nanoparticles were applied to both cell lines. Finally, the molecular mechanism of anticancer action of functionalised organoclays was elucidated.

Glycosylation of anthocyanins enhances the apoptosis of colon cancer cells by handicapping energy metabolism

“…The effect of BAE was evaluated on MC38 tumour cells and normal cells. As L929 cells are frequently used as normal control cells to detect apoptotic effects [ 30 – 32 ], we used L929 cells as the model of normal cells. According to the data in the literature [ 2 , 33 , 34 ] and the experimental results of IC50 (Table S 2 ), four doses (100, 200, 500 and 1000 μM) of ANC were used for the MTT assays.…”

Synthesis and Characterization of Folate Conjugated Boron Nitride Nanocarriers for Targeted Drug Delivery

“…Folic acid or its conjugates reversibly bind with the FRs, which are abundantly present on the cancer cell surface and easily penetrate through cellular membrane by receptor mediated endocytosis. , Recently, it has been reported that FA successfully forms conjugates with quantum dots, iron oxide NPs, gold NPs, and others. Such conjugates have been widely used in cancer cell targeting, tumor imaging, and drug delivery. − ,− ,− …”

“…Rzayev et al described the synthesis of antibacterial polymer thin films containing silver cations. Intercalated nanocomposites, in which FA conjugated with octadecyl amine-montmorillonite (ODA-MMT) and Ag-montmorillonite (Ag-MMT) due to electrostatic interactions, formed multilayer composite . The therapeutic effect demonstrated on L929 fibroblasts and DLD-1 cancer cells was achieved via interaction of amine groups with DNA–COOH sites and complexing of DNA with silver NPs under the formation of DNA–COOH···Ag complexes.…”

“…Another interesting approach is to use noncovalent attachment of FA to the NP surface. Rzayev et al described the synthesis of antibacterial polymer thin films containing silver cations. Intercalated nanocomposites, in which FA conjugated with octadecyl amine-montmorillonite (ODA-MMT) and Ag-montmorillonite (Ag-MMT) due to electrostatic interactions, formed multilayer composite .…”

“…Intercalated nanocomposites, in which FA conjugated with octadecyl amine-montmorillonite (ODA-MMT) and Ag-montmorillonite (Ag-MMT) due to electrostatic interactions, formed multilayer composite. 33 The therapeutic effect demonstrated on L929 fibroblasts and DLD-1 cancer cells was achieved via interaction of amine groups with DNA−COOH sites and complexing of DNA with silver NPs under the formation of DNA−COOH•••Ag complexes. The latter altered the DNA−protein and protein−protein interactions by destroying the self-assemble structural behaviors and cellular functions of DNA macromolecules.…”