Folic acid binding protein in acute myeloid leukaemia.

Gorst, D. W.; Courtis, Mary; Delamore, I. W.

doi:10.1136/jcp.29.1.60

Cited by 7 publications

(4 citation statements)

References 10 publications

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“…Previous studies have shown that folate-binding protein maybe actively synthesised at a greater than normal rate by chronic granulocytic leukaemia cells (Da Costa and Rothenberg, 1974b). In AML leucocytes, the level of folate binding was invariably normal, even though Gorst et al (1976) found folate binding to be raised in the sera of patients with AML. The exact cause of this increased serum binding in AML remains to be determined.…”

Section: Discussionmentioning

confidence: 91%

“…Subsequently, a folate binding protein was found in granulocytes of women who were pregnant or taking oral contraceptives (Da Costa and Rothenberg, 1974a) and in the sera of normal subjects and patients with a variety of diseases (Rothenberg, 1973;Waxman and Schreiber, 1973;Zettner and Duly, 1974;Colman and Herbert, 1976;Gorst et al, 1976;Rothenberg and Da Costa, 1976).…”

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confidence: 99%

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Specific and non-specific folate binding protein in normal and malignant human tissues

et al. 1978

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Binding of tritiated folic acid by supernatants prepared from extracts of normal and leukaemic leucocytes, normal mucosa, and malignant tumours from different parts of the gastrointestinal tract has been measured using Sephadex-gel filtration and albumin-coated charcoal techniques. Non-specific binding (measured by Sephadex G-75 gel filtration) was almost invariably greater than specific binding measured by albumin-coated charcoal separation of bound and unbound folate. In nine normal leucocyte extracts, binding measured by Sephadex G-75 filtration ranged from 1.3 to 18.2 (mean 8.2) pg/mg protein and by albumin-coated charcoal from 1.0 to 14.8 (mean 6.7) pg/mg protein. Raised specific binding was found in the extracts from leucocytes of eight of 14 patients with chronic granulocytic leukaemia, in four substantially so (389, 121, 108, 59.7 pg/mg protein), but was only marginally increased in one of eight cases of acute myeloid leukaemia and in two of five cases of chronic lymphocytic leukaemia. Binding was normal in the extracts of all three cases of acute lymphoblastic leukaemia tested. Among the tissues of the gastrointestinal tract binding was greatest by the duodenal mucosa and liver. Extracts of carcinoma of the stomach and colon bound greater amounts of (3)H-folic acid than the corresponding normal mucosal extracts but the differences were not large. Sephadex G-200 gel chromatography showed more than one binding peak in the extracts of liver and duodenum but only one peak in the other tissues of the gastrointestinal tract, and only one peak, of molecular weight either about 50 000 or over 200 000, in the leucocyte extracts.

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Section: Discussionmentioning

confidence: 91%

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confidence: 99%

Specific and non-specific folate binding protein in normal and malignant human tissues

et al. 1978

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“…FABP was measured as in the previous study [3]. Heparinised blood was separated into its various cell types by the method of Summers et at.…”

Section: Methodsmentioning

confidence: 99%

“…In an earlier study [3], 6 normal females were found to have levels in excess of 300 pg/ml whilst the mean level of 94 controls was 76 pg/ml. Sera and leucocyte lysates from these people have been used to esti mate the molecular weight of FABP in normal people.…”

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confidence: 99%

A Study of Folic-Acid-Binding Protein in Normal Subjects

Gorst¹,

Courtis²,

Delamore³

1977

Acta Haematol

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6 normal females were found to have a high level of folic-acid-binding protein (FABP) in their sera (mean level 345 pg/ml). Lysates of their various leucocytes were assayed and FABP found exclusively in the polymorph fraction. The sera and lysates were fractionated by Sephadex G-200 gel column chromatography and the molecular weight of the FABP appeared to be approximately 40,000. A hitherto unreported inhibitory effect of heparin on folate binding was noticed. Studies have been performed on folic-acid-binding protein (FABP) in various pathological states, but the levels found in normal people are usually considered too low to permit investigation. In an earlier study [3], 6 normal females were found to have levels in excess of 300 pg/ml whilst the mean level of 94 controls was 76 pg/ml. Sera and leucocyte lysates from these people have been used to estimate the molecular weight of FABP in normal people.

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The Interaction of Folate Ligands with Macromolecules

Costa

Rothenberg

1977

The Year in Hematology

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Folic acid binding protein in acute myeloid leukaemia.

Cited by 7 publications

References 10 publications

Specific and non-specific folate binding protein in normal and malignant human tissues

Specific and non-specific folate binding protein in normal and malignant human tissues

A Study of Folic-Acid-Binding Protein in Normal Subjects

The Interaction of Folate Ligands with Macromolecules

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