Foliar treatments with Gaultheria procumbens essential oil induce defense responses and resistance against a fungal pathogen in Arabidopsis

Vergnes, Sophie; Ladouce, Nathalie; Fournier, Sylvie; Ferhout, Hicham; Attia, Faouzi; Dumas, Bernard

doi:10.3389/fpls.2014.00477

Cited by 34 publications

(22 citation statements)

References 43 publications

(54 reference statements)

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“…Then, 340 nL of preamplified cDNA were used for qPCR array analysis in a 6.7 μL reaction using EvaGreen chemistry (Applied Biosystems). Data were analyzed with the BioMark Real-Time PCR Analysis Software Version 2.0 (Fluidigm) (Vergnes et al, 2014). Calculations for comparing expression data were performed using the 2 -ΔCt and the 2 -ΔΔCt methods (Livak and Schmittgen, 2001) using means Ct values of three M. truncatula reference genes which were selected using the NormFinder software (Andersen et al, 2004): Histone-3-like (Medtr4g097170), Ubiquitin family protein (Medtr3g097170) and Translation elongation factor 1 (Medtr6g021800) were used to standardize expression in seedlings.…”

Section: Methodsmentioning

confidence: 99%

MtNF-YA1, A Central Transcriptional Regulator of Symbiotic Nodule Development, Is Also a Determinant of Medicago truncatula Susceptibility toward a Root Pathogen

et al. 2016

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Plant NF-Y transcription factors control a wide array of biological functions enabling appropriate reproductive and developmental processes as well as adaptation to various abiotic and biotic environments. In Medicago truncatula, MtNF-YA1 was previously identified as a key determinant for nodule development and establishment of rhizobial symbiosis. Here, we highlight a new role for this protein in compatibility to Aphanomyces euteiches, a root pathogenic oomycete. The Mtnf-ya1-1 mutant plants showed better survival rate, reduced symptoms, and increased development of their root apparatus as compared to their wild-type (WT) background A17. MtNF-YA-1 was specifically up-regulated by A. euteiches in F83005.5, a highly susceptible natural accession of M. truncatula while transcript level remained stable in A17, which is partially resistant. The role of MtNF-YA1 in F83005.5 susceptibility was further documented by reducing MtNF-YA1 expression either by overexpression of the miR169q, a microRNA targeting MtNF-YA1, or by RNAi approaches leading to a strong enhancement in the resistance of this susceptible line. Comparative analysis of the transcriptome of WT and Mtnf-ya1-1 led to the identification of 1509 differentially expressed genes. Among those, almost 36 defense-related genes were constitutively expressed in Mtnf-ya1-1, while 20 genes linked to hormonal pathways were repressed. In summary, we revealed an unexpected dual role for this symbiotic transcription factor as a key player in the compatibility mechanisms to a pathogen.

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Section: Methodsmentioning

confidence: 99%

MtNF-YA1, A Central Transcriptional Regulator of Symbiotic Nodule Development, Is Also a Determinant of Medicago truncatula Susceptibility toward a Root Pathogen

et al. 2016

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“…Similar results were observed when A. thaliana was treated with Gaultheria procumbens L. EO, inducing an SA-mediated defense response and resistance to Colletotrichum higginsianumin Sacc. (64). However, these authors demonstrate that this effect is mainly due to the methyl salicylate present in the used EO, which triggered the SA-mediated defense response.…”

Section: Resultsmentioning

confidence: 99%

Oregano essential oil vapour preventsPlasmopora viticolainfection in grapevine (Vitis Vinifera) by triggering autoimmune metabolic pathways

Rienth

Crovadore

Ghaffari

et al. 2019

Preprint

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The reduction of synthetic fungicides in agriculture a major challenge in maintaining sustainable production, protecting the environment and consumers’ health. Downy mildew caused by the oomycete Plasmopora viticola is the major pathogen in viticulture worldwide and responsible for up to 60% of pesticide treatments. Alternatives to reduce fungicides are thus utterly needed to ensure sustainable vineyard-ecosystems, consumer health and public acceptance. Essential oils (EOs) are amongst the most promising natural plant protection alternatives and have shown their antibacterial, antiviral and antifungal properties on several agricultural crops. However, the efficiency of EOs highly depends on timing, application method and the molecular interactions between the host, the pathogen and EO. Despite proven EO efficiency, the underlying processes are still not understood and remain a black box. The objectives of the present study were: a) to evaluate whether a continuous fumigation of a particular EO can control downy mildew in order to circumvent the drawbacks of direct application, b) to decipher molecular mechanisms that could be triggered in the host and the pathogen by EO application and c) to try to differentiate whether essential oils directly repress the oomycete or act as plant resistance primers.A custom-made climatic chamber was used for a continuous fumigation of potted vines with different EOs during long-term experiments. The grapevine (Vitis vinifera) cv Chasselas was chosen in reason of its high susceptibility to Plasmopara viticola. Grapevine cuttings were infected with P. viticola. and subsequently exposed to continuous fumigation of different EOs at different concentrations, during 2 application time spans (24 hours and 10 days). Experiments were stopped when infection symptoms were clearly observed on the leaves of the control plants. Plant physiology (photosynthesis and growth rate parameters) were recorded and leaves were sampled at different time points for subsequent RNA extraction and transcriptomics analysis. Strikingly, the Oregano vulgare essential oil vapour treatment during 24h post-infection proved to be sufficient to reduce downy mildew development by 95%. Total RNA was extracted from leaves of 24h and 10d treatments and used for whole transcriptome shotgun sequencing (RNA-seq). Sequenced reads were then mapped onto the V. vinifera and P. viticola genomes. Less than 1% of reads could be mapped onto the P. viticola genome from treated samples, whereas up to 30 % reads from the controls mapped onto the P. viticola genome, thereby confirming the visual observation of P. viticola absence in the treated plants. On average, 80 % of reads could be mapped onto the V. vinifera genome for differential expression analysis, which yielded 4800 modulated genes. Transcriptomic data clearly showed that the treatment triggered the plant’s innate immune system with genes involved in salicylic, jasmonic acid and ethylene synthesis and signaling, activating Pathogenenesis-Related-proteins as well as phytoalexin synthesis.These results elucidate EO-host-pathogen interactions for the first time and indicate that the antifungal efficiency of EO is mainly due to the triggering of resistance pathways inside the host plants. This is of major importance for the production and research on biopesticides, plant stimulation products and for resistance-breeding strategiesAuthor SummaryThe reduction of synthetic plant protection products is a major concern of modern agriculture. The oomycetePlasmopora viticolawhich causes downy mildew in grapevine is amongst the most important grapevine pests and responsible for the dispersion of huge amounts of pesticides in vineyards. Among the evaluated alternatives to reduce or replace synthetic pesticides, plant volatile compounds could represent a sustainable solution. Some plant essential oils (EOs) have already shown antifungal capacities. However, their application is often difficult in terms of the right timing of treatment, degradation, bad rainfastness, mixability and phytotoxicity.The aim of the present work was to investigate whether the vapour phase, applied by a continuous fumigation of different EOs, might inhibit the development of downy mildew on grapevine, and in case of proven efficiency, to study the induced transcriptomic changes by RNA-sequencing in an attempt to elucidate the underlaying molecular interactions. Our results showed that the vapour phases ofO. vulgareandT. vulgariswere highly efficient against the pathogen. The study of differentially expressed genes indicated that the EO vapour triggered the main mechanisms of the plant’s innate immune system such as PTI (Pattern-Triggered Immunity) and ETI (Effector Triggered immunity).For the first time these results highlight the effects of EOs vapour on plant genes expression, which is very valuable information for the development of new natural plant protection products, as well as for breeding disease resistant cultivars.

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“…The plant molecular response to MeSA has been studied into essential oil extracts from Gaultheria procumbent (GEO), whose metabolic profile has been characterized recently [77]. GEO induced defense response against the hemibiotrophic fungus Colletotrichum higginsianum and was very effective in inducing SA plant defense-related genes similarly to the synthetic MeSA and also induced some marker genes of JA pathway [78]. A recent study investigated the role of volatile organic compounds inducing systemic acquired resistance (SAR).…”

Section: Plant Signaling Pathways and Eosmentioning

confidence: 99%

Antifungal Effect of Essential Oils

Arraiza¹,

González-Coloma⊥²,

Andrés³

et al. 2018

Potential of Essential Oils

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Essential oils are employed in agriculture, medicine and food industries among others, due to their antimicrobial, antiviral, insecticidal and antifungal properties. In this chapter, we will focus on the control of fungal plant pathogens with essential oils. Fungal diseases in agricultural crops and forestry alter the physiology of plants, disrupting their normal functioning, reducing their yield and sometimes causing their death. Recent studies show antifungal effects of many essential oils against plant pathogenic fungi, which make them candidates for the development of new fungicidal agents. This chapter presents a review of the most recent advances in this area, as well as the future trends in this field.

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Foliar treatments with Gaultheria procumbens essential oil induce defense responses and resistance against a fungal pathogen in Arabidopsis

Cited by 34 publications

References 43 publications

MtNF-YA1, A Central Transcriptional Regulator of Symbiotic Nodule Development, Is Also a Determinant of Medicago truncatula Susceptibility toward a Root Pathogen

MtNF-YA1, A Central Transcriptional Regulator of Symbiotic Nodule Development, Is Also a Determinant of Medicago truncatula Susceptibility toward a Root Pathogen

Oregano essential oil vapour preventsPlasmopora viticolainfection in grapevine (Vitis Vinifera) by triggering autoimmune metabolic pathways

Antifungal Effect of Essential Oils

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