Foliar spray of Si and Ti nanoparticles affected enzymatic antioxidants in rapeseed (Brassica napus L.)

Abstract

Some beneficial nanoparticles such as silicon and titanium are encouraging in foliar feeding due to their large surface area and biocompatible properties. In current experiment, seedling of rapeseed varieties (‘Hydromel’, ‘Nathalie’, ‘Alonso’) was sprayed with titanium dioxide and silicon dioxide nanoparticles (Ti and Si-NPs) and then responses of some enzymatic antioxidant and plasma membrane integrity were evaluated. Foliar application of Si-NPs significantly increased the activity of ascorbate peroxidase compared to the control, in all three varieties. Spray of both Ti and Si-NPs increased guaiacol peroxidase activity in ‘Nathalie’ and ‘Alonso’ varieties while in ‘Hydromel’ only Si-NPs spray had positive effects on activity this antioxidant. Behaviors of superoxide dismutase (SOD) isozymes under different foliar treatments and in varieties were different, but the highest activity of total SOD isozyme in ‘Hydromel’ and ‘Alonso’ varieties was obtained by foliar application of Si-NPs and Ti-NPs, respectively. The lowest amount of hydrogen peroxide was obtained by foliar application of Ti-NPs. Examination of malondialdehyde (MDA) also showed that the most vulnerable membrane belonged to ‘Hydromel’ variety and foliar application of Si-NPs and Ti-NPs could improve membrane integrity. These results suggest that foliar application of both Si-NPs and Ti-NPs improves the scavenging capacity of reactive oxygen species (ROS), although the response of the enzymatic antioxidants was largely influenced by the variety.

Physiochemical response of Cicer arietinum to zinc-containing mesoporous silica nanoparticles under water stress

“…Zinc, as a cofactor of many key enzymes, regulates the biosynthesis of phytohormones such as auxin and abscisic acid. Silicon also plays a role as a beneficial element in stimulating defense and regulatory processes (Spalevic et al, 2022 ). Our findings are consistent with those of El-Zohri et al (2021), who reported that foliar spraying of green-synthesized Zn-NPs induced SOD, CAT, and APX activities, and reduced drought-induced oxidative stress, as indicated by the decreased levels of MDA and H 2 O 2 compared to untreated controls.…”

“…Karami et al (2016) observed increased activity of antioxidant enzymes, including superoxide dismutase, peroxidase, CAT, and proline, in soybeans following the foliar application of zinc under drought-stress conditions. Silicon, while nonessential for plant growth, has demonstrated beneficial effects when applied exogenously to various plant species (Liang et al, 2015; Spalevic et al, 2022 ). The application of silicon under drought stress conditions improves plant tolerance through multiple mechanisms, such as regulating aquaporin gene expression and reducing ROS, which inhibit aquaporin activity (Wang et al, 2021 ).…”

The Effect of Microorganisms and Inorganic Preparations Applied into the Soil and Leaves on the Yield of Winter Rape (<i>Brassica Napus</i> L.)

“…Over the last decade, a significant increase in the use of biostimulators has been observed in agricultural practice. In modern agriculture, foliar and soil application of nutrients is becoming a commonly used procedure (Kolarić et al 2021;Spalevic et al 2022), but both new and known preparations in agricultural practice should be tested under field experiments (Beres et al 2019, Rios et al 2019.…”