Foliar-Anatomical Adaptations of Micropropagated Plants of Dendrocalamus strictus (Roxb.) Nees Towards Photoautotrophic Conditions

Shekhawat, Mahipal S.; Rajput, Bharat S.; Manokari, M.

doi:10.1007/978-981-16-1310-4_6

Cited by 2 publications

(4 citation statements)

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“…2021a; Shekhawat et al . 2021a). Light microscopy can help to understand the structural plasticity of plants to changed environmental conditions, which could improve the in vivo survival of tissue culture‐raised plantlets.…”

Section: Resultsmentioning

confidence: 99%

“…2020; Shekhawat et al . 2021a). These abnormal features of micro‐propagated plants restrict control of water loss and increase evapotranspiration when subjected to sudden changes in environmental conditions during transfer to ex vitro or in vivo conditions (Teixeira da Silva et al .…”

Section: Resultsmentioning

confidence: 99%

“…The physical environment used for ex vitro plantlets affects the structural and functional development of micropropagated plantlets (Rodrigues et al 2014;Wang et al 2020). The structural and physiological adaptation of plantlets begins with development of the dermis (thick cuticle, uniform epidermal layers, reduced stomatal density), ground tissue (well-developed mesophyll with chlorophyll and organized parenchyma around vascular tissues) and vascular systems (increased xylem and phloem tissue density) that are catalysed by enzymes in the cells and regulated by photoautotrophic light conditions (Manokari et al 2021a;Shekhawat et al 2021a). Light microscopy can help to understand the structural plasticity of plants to changed environmental conditions, which could improve the in vivo survival of tissue culture-raised plantlets.…”

Section: Resultsmentioning

confidence: 99%

“…Problems associated with the growth environment and stages of plantlet development can usually be recognized during microscopic examination of essential organs (particularly, leaves and roots) at appropriate stages of development (Sharma et al 2017;Revathi et al 2018;Manokari et al 2021aManokari et al , 2021b. Therefore, a better understanding of the complex effects of physiochemical factors on plant micro-morpho-anatomical development during in vitro and ex vitro plantlet formation will result in successful survival of plants under field conditions (Manokari et al 2021a;Shekhawat et al 2021aShekhawat et al , 2021b.…”

Section: Introductionmentioning

confidence: 99%

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Micro‐morpho‐anatomical transitions at various stages of in vitro development of Crinum malabaricum Lekhak and Yadav: A critically endangered medicinal plant

et al. 2022

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Crinum malabaricum Lekhak & Yadav is a recently discovered and critically endangered aquatic bulbous plant of the family Amaryllidaceae. It gained attention as a wild source of the acetylcholinesterase inhibiting alkaloid 'galanthamine' used to treat Alzheimer and Parkinson diseases. The bulbs of this plant contain the highest amount of galanthamine among Crinum species.• In vitro regeneration systems were developed to produce quality uniform plantlets of C. malabaricum. Bright field light microscopy was used to analyse micro-morphoanatomical developments taking place in the leaves and roots during in vitro, ex vitro and in vivo transitions of plantlets.• Leaves and roots of plants raised in vitro possessed a higher degree of microscopic structural anomalies, such as underdeveloped epicuticular wax deposition, immature and non-functional stomata, more aquiferous parenchyma with a reduced lumen. Roots developed in vitro were characterized by extremely large, uneven cortical cells and reduced intercellular spaces. The vascular tissues were under-developed and only primary vascular tissues were observed. As a result of ex vitro acclimation, there was a significant acceleration in the improvement of tissue systems in leaves and roots. Such plantlets can tolerate elevated temperatures and light under in vivo conditions.• Thus, the microscopic evaluation of the structural trajectory in different stages of plantlet development provides an understanding of the acclimation process and structural adaptations, which could help enhance survival of in vitro raised plantlets under ex vitro and in vivo conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%