FolE gene expression for folic acid productivity from optimized and characterized probiotic Lactobacillus delbrueckii

Khedr, Mohamed; Youssef, Fady Sayed; El-kattan, Noura; Abozahra, Mahmoud S.; Selim, Mohammed N.; Yousef, Abdullah; Khalil, Kamal M.A.; Mekky, Alsayed E.

doi:10.1186/s43141-023-00603-9

Cited by 2 publications

(1 citation statement)

References 50 publications

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“…Similarly, Abdelrazik et al [ 104 ] used 16S rDNA PCR sequencing to identify the top five MDR bacterial isolates: Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, Enterococcus faecalis , and Klebsiella pneumoniae, with accession numbers OP741103, OP741104, OP741105, OP741106, and OP741107, respectively. Other research conducted by Khedr et al [ 105 ] included the identification of three LAB isolates as Lactobacillus delbrueckii using 16S rDNA.…”

Section: Discussionmentioning

confidence: 99%

Unravelling the Antimicrobial, Antibiofilm, Suppressing Fibronectin Binding Protein A (fnba) and cna Virulence Genes, Anti-Inflammatory and Antioxidant Potential of Biosynthesized Solanum lycopersicum Silver Nanoparticles

Mekky,

Abdelaziz,

Youssef

et al. 2024

Medicina

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Background and Objectives: Urinary tract infections [UTIs] are considered the third most known risk of infection in human health around the world. There is increasing appreciation for the pathogenicity of Gram-positive and Gram-negative strains in UTIs, aside from fungal infection, as they have numerous virulence factors. Materials and Methods: In this study, fifty urine samples were collected from patients suffering from UTI. Among the isolates of UTI microbes, six isolates were described as MDR isolates after an antibiotic susceptibility test carried out using ten different antibiotics. An alternative treatment for microbial elimination involved the use of biosynthesized silver nanoparticles (AgNPs) derived from Solanum lycopersicum [S. cumin]. Results: The sizes and shapes of AgNPs were characterized through TEM imaging, which showed spherical particles in a size range of 35–80 nm, of which the average size was 53 nm. Additionally, the silver nanoparticles (AgNPs) demonstrated inhibitory activity against Staphylococcus aureus (OR648079), exhibiting a 31 mm zone of inhibition at a minimum inhibitory concentration (MIC) of 4 mg/mL and a minimum bactericidal concentration (MBC) of 8 mg/mL. This was followed by Aspergillus niger (OR648075), which showed a 30 mm inhibition zone at an MIC of 16 mg/mL and a minimum fungicidal concentration (MFC) of 32 mg/mL. Then, Enterococcus faecalis (OR648078), Klebsiella pneumoniae (OR648081), and Acinetobacter baumannii (OR648080) each displayed a 29 mm zone of inhibition at an MIC of 8 mg/mL and an MBC of 16 mg/mL. The least inhibition was observed against Candida auris (OR648076), with a 25 mm inhibition zone at an MIC of 16 mg/mL and an MFC of 32 mg/mL. Furthermore, AgNPs at different concentrations removed DPPH and H2O2 at an IC50 value of 13.54 μg/mL. Also, AgNPs at 3 mg/mL showed remarkable DNA fragmentation in all bacterial strains except Enterococcus faecalis. The phytochemical analysis showed the presence of different active organic components in the plant extract, which concluded that rutin was 88.3 mg/g, garlic acid was 70.4 mg/g, and tannic acid was 23.7 mg/g. Finally, AgNPs concentrations in the range of 3–6 mg/mL showed decreased expression of two of the fundamental genes necessary for biofilm formation within Staphylococcus aureus, fnbA (6 folds), and Cna (12.5 folds) when compared with the RecA gene, which decreased by one-fold when compared with the control sample. These two genes were submitted with NCBI accession numbers [OR682119] and [OR682118], respectively. Conclusions: The findings from this study indicate that biosynthesized AgNPs from Solanum lycopersicum exhibit promising antimicrobial and antioxidant properties against UTI pathogens, including strains resistant to multiple antibiotics. This suggests their potential as an effective alternative treatment for UTIs. Further research is warranted to fully understand the mechanisms of action and to explore the therapeutic applications of these nanoparticles in combating UTIs.

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Section: Discussionmentioning

confidence: 99%

Unravelling the Antimicrobial, Antibiofilm, Suppressing Fibronectin Binding Protein A (fnba) and cna Virulence Genes, Anti-Inflammatory and Antioxidant Potential of Biosynthesized Solanum lycopersicum Silver Nanoparticles

Mekky,

Abdelaziz,

Youssef

et al. 2024

Medicina

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Hematological profile, rumen fermentation, antioxidant state, and immune response of Egyptian Nubian goats fed on Astragalus membranaceus root extract supplemented ration

Oraby,

Youssef,

El-Sherif

et al. 2024

Egyptian Pharmaceutical Journal

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Background In recent years, Astragalus membranaceus extract has been widely used in animals due to their antimicrobial activities, ability to enhance immunity, and antioxidant functions. Objective We aimed to determine the antioxidant and inhibitory activities of A. membranaceus root powder (AMP) and its cytotoxicity and effects on hematological profile, rumen fermentation, antioxidant status, and immune response in Egyptian Nubian goats. Materials and methods Twenty-five goats are used in this study were received 20 g/animal/day of AMP mixed with their basic diet for 28 days. The study involved measuring the antioxidant activity of AMP using the 2-diphenyl-1-picrylhydrazyl radical scavenging assay and determining the viability and cytotoxicity percentage using the methyl-thiazolyl tetrazolium protocol. Results After 14 and 28 days of the daily feeding with 20 g of AMP, there was a significant increase in hematological profile, leukocyte count, total volatile fatty acid, and rumen ammonium concentrations with an enhancement in protozoal activity. Also, there was an increase in catalase and total antioxidant capacities along with promoting immunoglobulin (A, M, and G) contents with no significant effect on the insulin level compared with 0 days. Malondialdehyde contents decreased significantly. For all examined concentrations, A. membranaceus showed antioxidant and anti-inflammatory activities. It also showed a high cytotoxicity percentage in cancer cells. Discussion and conclusions A. membranaceus root extract supplementation significantly increases hematology parameters and rumen fermentation, and improves immune status and antioxidant activity both in-vitro and in live animals. It also exhibits potent cytotoxicity on cancer cells.

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FolE gene expression for folic acid productivity from optimized and characterized probiotic Lactobacillus delbrueckii

Cited by 2 publications

References 50 publications

Unravelling the Antimicrobial, Antibiofilm, Suppressing Fibronectin Binding Protein A (fnba) and cna Virulence Genes, Anti-Inflammatory and Antioxidant Potential of Biosynthesized Solanum lycopersicum Silver Nanoparticles

Unravelling the Antimicrobial, Antibiofilm, Suppressing Fibronectin Binding Protein A (fnba) and cna Virulence Genes, Anti-Inflammatory and Antioxidant Potential of Biosynthesized Solanum lycopersicum Silver Nanoparticles

Hematological profile, rumen fermentation, antioxidant state, and immune response of Egyptian Nubian goats fed on Astragalus membranaceus root extract supplemented ration

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