Folding Dynamics of Ferrocytochrome c in a Denaturant‐Free Environment Probed by Transient Grating Spectroscopy

Regulation of Protein Structural Changes by Incorporation of a Small-Molecule Linker

“…TA measurement was performed by following previously reported procedures [26,27,28,29]. A pump beam (460 nm) was produced by an optical parametric oscillator (LT-2214-PC, LOTIS, Minsk, Belarus) pumped by a third harmonics (355 nm) of an Nd:YAG laser (NL 301G, EKSPLA, Vilnius, Lithuania).…”

The time scale of the quaternary structural changes in hemoglobin revealed using the transient grating technique

“…[29][30][31][32][33][34][35][36][37][38][39][40] Before using the TG technique, for comparison with previous studies, we first measured the TA spectra of HbCO in 100 mM sodium phosphate buffer (pH 7.0) and observed four relaxation times of 1.2 ms, 48 ms, 310 ms, and 6.0 ms (Fig. One of the key questions is which kinetics within this broad time window is primarily responsible for the quaternary structural change associated with the R-T transition.…”

“…[29][30][31][32][33][34][35][36][37][38][39][40] Theoretically, the species grating (dn spe ) arises from the change in the absorption spectrum (population grating, dn p ) and the change in the molecular volume (volume grating, dn v ), as follows: dn spe = dn p + dn v . It is known that the TG signal is very sensitive to the volume change induced by the conformational change of a protein.…”

“…To address this question, herein we used the TG technique, which is a useful tool for sensitively probing the global structural change of a protein by monitoring changes in the diffusion coefficient. [29][30][31][32][33][34][35][36][37][38][39][40] Before using the TG technique, for comparison with previous studies, we first measured the TA spectra of HbCO in 100 mM sodium phosphate buffer (pH 7.0) and observed four relaxation times of 1.2 ms, 48 ms, 310 ms, and 6.0 ms (Fig. S1, ESI †).…”

“…It is known that the TG signal is very sensitive to the volume change induced by the conformational change of a protein. [29][30][31][32][33][34][35][36][37][38][39][40] Theoretically, the species grating (dn spe ) arises from the change in the absorption spectrum (population grating, dn p ) and the change in the molecular volume (volume grating, dn v ), as follows: dn spe = dn p + dn v . The volume grating is expressed by Therefore, based on the principles of the TG measurements, we suggest that the dynamics at 1.7 ms observed in the TG experiments is due to the quaternary structural change accompanied by the R-T transition, which leads to a large volume change.…”