Folate-Polyethylene Glycol Conjugated Near-Infrared Fluorescence Probe with High Targeting Affinity and Sensitivity for In Vivo Early Tumor Diagnosis

Liu, Fei; Deng, Dawei; Chen, Xinyang; Qian, Zhiyu; Achilefu, Samuel; Gu, Yueqing

doi:10.1007/s11307-010-0305-1

Cited by 64 publications

(50 citation statements)

References 47 publications

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“…To normalize fluorescence intensity from the plate reader the highest peak intensity was taken to be “100”. Based on NMR measurements, the folate/PEG and mannose/PEG conjugation efficiencies was >90% which concurs with previous work [24]. …”

Section: Methodssupporting

confidence: 92%

Development of optical probes for in vivo imaging of polarized macrophages during foreign body reactions

et al. 2014

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Plasticity of macrophages (MΦ) phenotypes exist in a spectrum from classically activated (M1) cells, to alternatively activated (M2) cells, contributing to both the normal healing of tissues and the pathogenesis of implant failure. Here, folate- and mannose-based optical probes were fabricated to simultaneously determine the degree of MΦ polarization. In vitro tests show the ability of these probes to specifically target M1 and M2 cells. In an in vivo murine model, they were able to distinguish between M1-dominated inflammatory response to infection and M2-dominated regenerative response to particle implants. Finally, the probes were used to assess the inflammatory/ regenerative property of biomaterial implants. Our results show that these probes can be used to monitor and quantify the dynamic processes of MΦ polarization and their role in cellular responses in real time.

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Section: Methodssupporting

confidence: 92%

Development of optical probes for in vivo imaging of polarized macrophages during foreign body reactions

et al. 2014

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“…KB cells are human epidermal carcinoma cell line from oral cavity on which folate receptor is over-expressed [23]–[25]. A549 cells are human lung adenocarcinoma epithelial cell line on which folate receptor is deficient [23], [24], [26]. HepG2 cells are human liver tumor cell line [27], [28].…”

Section: Methodsmentioning

confidence: 99%

Cellular Uptake and Antitumor Activity of DOX-hyd-PEG-FA Nanoparticles

Zhang

et al. 2014

PLoS ONE

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A PEG-based, folate mediated, active tumor targeting drug delivery system using DOX-hyd-PEG-FA nanoparticles (NPs) were prepared. DOX-hyd-PEG-FA NPs showed a significantly faster DOX release in pH 5.0 medium than in pH 7.4 medium. Compared with DOX-hyd-PEG NPs, DOX-hyd-PEG-FA NPs increased the intracellular accumulation of DOX and showed a DOX translocation from lysosomes to nucleus. The cytotoxicity of DOX-hyd-PEG-FA NPs on KB cells was much higher than that of free DOX, DOX-ami-PEG-FA NPs and DOX-hyd-PEG NPs. The cytotoxicity of DOX-hyd-PEG-FA NPs on KB cells was attenuated in the presence of exogenous folic acid. The IC50 of DOX-hyd-PEG-FA NPs and DOX-hyd-PEG NPs on A549 cells showed no significant difference. After DOX-hyd-PEG-FA NPs were intravenously administered, the amount of DOX distributed in tumor tissue was significantly increased, while the amount of DOX distributed in heart was greatly decreased as compared with free DOX. Compared with free DOX, NPs yielded improved survival rate, prolonged life span, delayed tumor growth and reduced the cardiotoxicity in tumor bearing mice model. These results indicated that the acid sensitivity, passive and active tumor targeting abilities were likely to act synergistically to enhance the drug delivery efficiency of DOX-hyd-PEG-FA NPs. Therefore, DOX-hyd-PEG-FA NPs are a promising drug delivery system for targeted cancer therapy.

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“…5, there was minimal decrease in cell viability (<10%), even when HBl-100 cells was incubated with 400 μg/mL of FASOC-UCNP-ZnPc for 48 h. This cytotoxicity experiment demonstrated that FASOC-UCNPs were low toxicity without light irradiation. The observed low cytotoxicity could be attributed to the FASOC layer on the surface of UCNPs, which was little or no cytotoxicity, as demonstrated by many researchers [21,26,32] . …”

Section: Cytotoxicity Studymentioning

confidence: 79%

Preparation of multifunctional upconversion nanoconstruct for in vitro and in vivo imaging and photodynamic therapy induced by near-infrared light

et al. 2014

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Upconversion nanoparticles (UCNPs) have attracted much attention as novel contrast agents for low-background biomedical imaging and potential photosensitizer carriers for photodynamic therapy (PDT) in deep tissues. In this work, firstly we synthesized NaYF 4 :Yb 3+ ,Tm 3+ @NaYF 4 :Yb 3+ ,Er 3+ core-shell structured UCNPs with multiple emission peaks (e.g. 480, 539, 654 and 800 nm) by a seeded growth approach. By coating folate-modified amphiphilic chitosan (FASOC) on UCNPs, the as-prepared UCNPs were favorably endowed with good water solubility and the enhanced tumor-selectivity to cancer cells that overexpressed folate receptor. Then, water-insoluble photosensitizer zinc(II) phthalocyanine (ZnPc) was loaded into the FASOC-UCNPs via hydrophobic interactions for potential near-infrared light induced photodynamic therapy (PDT). Our results indicate that the multifunctional FASOC-UCNP-ZnPc nanoconstruct has efficient NIR-to-NIR upconversion luminescence and PDT abilities, which could be potentially employed as a theranostic platform for cancer treatment.

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Folate-Polyethylene Glycol Conjugated Near-Infrared Fluorescence Probe with High Targeting Affinity and Sensitivity for In Vivo Early Tumor Diagnosis

Abstract: fPI-01 is a promising optical agent for diagnosis of FR-positive tumors, especially in their early stage.

Cited by 64 publications

References 47 publications

Development of optical probes for in vivo imaging of polarized macrophages during foreign body reactions

Development of optical probes for in vivo imaging of polarized macrophages during foreign body reactions

Cellular Uptake and Antitumor Activity of DOX-hyd-PEG-FA Nanoparticles

Preparation of multifunctional upconversion nanoconstruct for in vitro and in vivo imaging and photodynamic therapy induced by near-infrared light

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