Folate mediated self-assembled phytosterol-alginate nanoparticles for targeted intracellular anticancer drug delivery

Wang, Jianting; Wang, Ming; Zheng, Mingming; Guo, Qiong; Wang, Yafan; Wang, Heqing; Xie, Xiangrong; Huang, Fenghong; Gong, Renmin

doi:10.1016/j.colsurfb.2015.03.028

Cited by 63 publications

(31 citation statements)

References 31 publications

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“…The most representative magnetic material at present is Fe 3 0 4 powder, which can not only provide magnetism for the targeting drugs, but can also load the drugs for targeted therapy (10). The drugs used in MTDDS currently include adriamycin (11), folic acid (12), cisplatin and insulin (13). The frame material is the part connecting the magnetic material and the drug, including glucans (14) and chitosan (15).…”

Section: Discussionmentioning

confidence: 99%

Safety and toxicology of the intravenous administration of Ang2-siRNA plasmid chitosan magnetic nanoparticles

Shan

Liu

et al. 2016

Molecular Medicine Reports

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This aim of the present study was to investigate the safety and toxicology of intravenous administration of angiopoietin-2 (Ang2)-small interfering (si)RNA plasmid-chitosan magnetic nanoparticles (CMNPs). Ang2-CMNPs were constructed and subsequently administered at different doses to mice and rats via the tail vein. The acute (in mice) and chronic toxicity (in rats) were observed. The results of the acute toxicity assay revealed that the LD50 mice was >707.0 mg·kg-1·d-1, and the general condition of mice revealed no obvious abnormalities. With the exception of the high dose group (254.6 mg·kg-1·d-1), which exhibited partial lung congestion, the other groups exhibited no obvious abnormalities. Results of the chronic toxicity assay demonstrated that the non-toxic dose of Ang2-CMNPs in the rat was >35.35 mg·kg-1·d-1 for 14 days. The rat general condition and blood biochemistry indexes revealed no obvious abnormality. The blood routine indexes and lung/body ratio of each treatment group were higher when compared with the control group. The middle- and high-dose groups exhibited chronic pulmonary congestion, whilst the low-dose and control groups exhibited no abnormality. Similarly, the other organs revealed no obvious abnormality. Ang2-CMNPs have good safety at a certain dose range and may be considered as the target drug carrier.

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Section: Discussionmentioning

confidence: 99%

Safety and toxicology of the intravenous administration of Ang2-siRNA plasmid chitosan magnetic nanoparticles

Shan

Liu

et al. 2016

Molecular Medicine Reports

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“…In vitro cellular tests showed that DOX‐loaded nanoparticles had a high binding affinity to the folate receptors and primarily located at the outer portion of the plasma membrane. It was observed that the nanoparticles are internalized by the folate receptor–mediated endocytosis mechanism, and this endocytosis pathway may result in a significant increase in the cellular uptake efficiency .…”

Section: Polysaccharide Nanoparticlesmentioning

confidence: 99%

Overviews on the cellular uptake mechanism of polysaccharide colloidal nanoparticles

Salatin

Khosroushahi

2017

J Cellular Molecular Medi

240

143

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Nanoparticulate drug/gene carriers have gained much attention in the past decades because of their versatile and tunable properties. However, efficacy of the therapeutic agents can be further enhanced using naturally occurring materials‐based nanoparticles. Polysaccharides are an emerging class of biopolymers; therefore, they are generally considered to be safe, non‐toxic, biocompatible and biodegradable. Considering that the target of nanoparticle‐based therapeutic strategies is localization of biomedical agents in subcellular compartments, a detailed understanding of the cellular mechanism involved in the uptake of polysaccharide‐based nanoparticles is essential for safe and efficient therapeutic applications. Uptake of the nanoparticles by the cellular systems occurs with a process known as endocytosis and is influenced by the physicochemical characteristics of nanoparticles such as size, shape and surface chemistry as well as the employed experimental conditions. In this study, we highlight the main endocytosis mechanisms responsible for the cellular uptake of polysaccharide nanoparticles containing drug/gene.

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“…Polymeric nanoparticles (NPs) to be developed as anticancer drug transporters are recently emerging because of the promise in both their protection of the drug from rapid metabolism or clearance and their selective accumulation in tumorous tissues via the enhanced permeability and retention (EPR) effect caused by the vascular architecture difference of tumorous tissue and the poor lymphatic drainage system [1]. Among a variety of polymeric NPs for targeting delivery of anticancer drugs, the NPs based on natural polysaccharides, such as heparin [2], dextran [3], curdlan [4], xyloglucan [5], arabinogalactan [6], hyaluronic acid [7,8], alginate [9][10][11][12], pullulan [13][14][15][16][17], and chitosan [18][19][20][21][22][23][24][25], have attracted the attention of pharmacologists. In particular, pullulan has aroused scientists' increasing interest due to its specific structure and outstanding biological properties, such as biocompatibility, biodegradability, low immunogenicity, nontoxicity, and water solubility.…”

Section: Introductionmentioning

confidence: 99%

Facile One-Pot Synthesis of Self-Assembled Folate-Biotin-Pullulan Nanoparticles for Targeted Intracellular Anticancer Drug Delivery

Wang

Huang

Wang

et al. 2016

Journal of Nanomaterials

Self Cite

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The self-assembled folate-biotin-pullulan (FBP) nanoparticles (NPs) were prepared by facile one-pot synthesis and their physicochemical properties were characterized. The self-assembled FBP NPs were used as an anticancer drug nanocarrier entrapping doxorubicin (DOX) for targeting folate-receptors-overexpressing cancer cells. The identification of prepared NPs to folate-receptor-expressing cancer cells (KB cells) was affirmed by cell viability measurement, folate competition test, and flow cytometric analysis. Compared with the naked DOX and DOX/BP NPs, the DOX/FBP NPs had lower IC50value compared to KB cells as a result of the folate-receptor-mediated endocytosis process. The cytotoxicity of DOX/FBP NPs to KB cells could be inhibited competitively by free folate. The cellular intake pattern of naked DOX and drug-loaded NPs was identified by confocal laser scanning microscopy (CLSM) observation and the higher cellular uptake of drug for DOX/FBP NPs over naked DOX was observed. The prepared FBP NPs had the potential to be used as a powerful carrier to target anticancer drugs to folate-receptor-expressing tumor cells and reduce cytotoxicity to normal tissues.

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Folate mediated self-assembled phytosterol-alginate nanoparticles for targeted intracellular anticancer drug delivery

Cited by 63 publications

References 31 publications

Safety and toxicology of the intravenous administration of Ang2-siRNA plasmid chitosan magnetic nanoparticles

Safety and toxicology of the intravenous administration of Ang2-siRNA plasmid chitosan magnetic nanoparticles

Overviews on the cellular uptake mechanism of polysaccharide colloidal nanoparticles

Facile One-Pot Synthesis of Self-Assembled Folate-Biotin-Pullulan Nanoparticles for Targeted Intracellular Anticancer Drug Delivery

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