Folate Functionalized and Evodiamine‐Loaded Pluronic Nanomicelles for Augmented Cervical Cancer Cell Killing

Solanki, Raghu; Jangid, Ashok Kumar; Jadav, Mahima; Kulhari, Hitesh; Patel, Sunita

doi:10.1002/mabi.202300077

Cited by 13 publications

(7 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cells were incubated with ENPs and fluorescence analysis was done by fluorescence microscope. The dyes AO and EtBr are used to distinguish between necrotic, apoptotic, and normal cells 30 . AO dye penetrates and emits green fluorescence when bound to the DNA of normal and healthy cells with intact membranes.…”

Section: Resultsmentioning

confidence: 99%

Enhancing apoptosis-mediated anticancer activity of evodiamine through protein-based nanoparticles in breast cancer cells

Solanki,

Rajput,

Jodha

et al. 2024

Sci Rep

Self Cite

View full text Add to dashboard Cite

In the cutting-edge era of developing precision therapeutics, nanoparticles have emerged as a potent drug delivery system. Altering the size of poorly water-soluble drugs to nanoscale could confer change in their physical properties, including enhanced water solubility and bioavailability. Evodiamine (EVO), a natural indolequinone alkaloid extract from Evodia rutaecarpa, has shown several important pharmacological applications, anti-cancer being one of them. Protein-based nano-drug delivery systems have gained the interest of researchers due to their better biocompatibility, biodegradability, non-immunogenicity and non-toxicity. In the present study, EVO encapsulated BSA nanoparticles (ENPs) were synthesized and characterized, which were nanoscale-sized (~ 150 nm), monodispersed, spherical shaped, and showed high entrapment efficiency (~ 86%) and controlled drug release. The in-vitro anti-cancer activity of ENPs on human breast cancer cells was dose- and time-dependent. The apoptotic molecular mechanism investigated using FACS, qRT-PCR, and western blotting analysis, revealed increased expression of p53 and Bax and decreased expression of Bcl-2. Biological studies demonstrated comparatively more efficient and targeted delivery of ENPs than pure EVO. The comprehensive physiochemical characterization and in-vitro validation collectively pinpoint ENPs as a promising avenue for harnessing the therapeutic potential of the natural anti-cancer compound EVO. The findings indicate improved cytotoxicity, positioning ENPs as a propitious strategy for advancing breast cancer treatment.

show abstract

Section: Resultsmentioning

confidence: 99%

Enhancing apoptosis-mediated anticancer activity of evodiamine through protein-based nanoparticles in breast cancer cells

Solanki,

Rajput,

Jodha

et al. 2024

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Solanki et al synthesized evodiamine-loaded folate-conjugated Pluronic F108 nanomicelles using the EDC/NHS reaction method to enhance evodiamine's efficacy against cervical cancer. These NDDS efficiently inhibited cell migration, augmented intracellular drug uptake, and induced apoptosis in HeLa cervical cancer cells (Solanki et al, 2023). For other alkaloid compounds see Table 5.…”

Section: Other Alkaloid Compoundsmentioning

confidence: 98%

The development of nanocarriers for natural products

Huang,

Luo,

Tong

et al. 2024

WIREs Nanomed Nanobiotechnol

View full text Add to dashboard Cite

Natural bioactive compounds from plants exhibit substantial pharmacological potency and therapeutic value. However, the development of most plant bioactive compounds is hindered by low solubility and instability. Conventional pharmaceutical forms, such as tablets and capsules, only partially overcome these limitations, restricting their efficacy. With the recent development of nanotechnology, nanocarriers can enhance the bioavailability, stability, and precise intracellular transport of plant bioactive compounds. Researchers are increasingly integrating nanocarrier‐based drug delivery systems (NDDS) into the development of natural plant compounds with significant success. Moreover, natural products benefit from nanotechnological enhancement and contribute to the innovation and optimization of nanocarriers via self‐assembly, grafting modifications, and biomimetic designs. This review aims to elucidate the collaborative and reciprocal advancement achieved by integrating nanocarriers with botanical products, such as bioactive compounds, polysaccharides, proteins, and extracellular vesicles. This review underscores the salient challenges in nanomedicine, encompassing long‐term safety evaluations of nanomedicine formulations, precise targeting mechanisms, biodistribution complexities, and hurdles in clinical translation. Further, this study provides new perspectives to leverage nanotechnology in promoting the development and optimization of natural plant products for nanomedical applications and guiding the progression of NDDS toward enhanced efficiency, precision, and safety.This article is categorized under: Therapeutic Approaches and Drug Discovery > Emerging Technologies Nanotechnology Approaches to Biology > Nanoscale Systems in Biology

show abstract

“…In particular, the pathogenesis of cancer is linked to a reduction in anti-inflammatory bacteria like Lactobacillus, Roseburia, Akkermansia muciniphila, and Eubacterium, and to an increase in the growth of proinflammatory species like Ruminococcus gnavus and Bacteroidetes. The mechanism at the base of dysbiosis is the break of gut-barrier integrity that promotes the translocation of bacteria fragments like lipopolysaccharide (LPS) to the blood, with consequences on systemic inflammation, oxidative state, cellular immunity, and metabolism (Figure 2) [19][20][21][22][23][24][25][26][27][28].…”

Section: Relationship Between Medical Plants Gut Microbiota and Cancermentioning

confidence: 99%

The Interplay between Medical Plants and Gut Microbiota in Cancer

et al. 2023

View full text Add to dashboard Cite

The gut microbiota is a dynamic community of bacteria distributed in the gastroenteric tract and changes in response to diseases, diet, use of antibiotics and probiotics, hygiene status, and other environmental factors. Dysbiosis, a disruption of the normal crosstalk between the host and the microbes, is associated with obesity, diabetes, cancer, and cardiovascular diseases, is linked to a reduction of anti-inflammatory bacteria like Lactobacillus and Roseburia, and to an increase in the growth of proinflammatory species like Ruminococcus gnavus and Bacteroidetes. Some plants possess anticancer properties and various studies have reported that some of these are also able to modulate the gut microbiota. The aim of this work is to evaluate the crucial relationship between medical plants and gut microbiota and the consequences on the onset and progression of cancer. In vivo studies about hematological malignancies showed that beta-glucans tie to endogenous antibeta glucan antibodies and to iC3b, an opsonic fragment of the central complement protein C3, leading to phagocytosis of antibody-targeted neoplastic cells and potentiation of the cytotoxic activity of the innate immune system if administered together with monoclonal antibodies. In conclusion, this review suggests the potential use of medical plants to improve gut dysbiosis and assist in the treatment of cancer.

show abstract

Folate Functionalized and Evodiamine‐Loaded Pluronic Nanomicelles for Augmented Cervical Cancer Cell Killing

Cited by 13 publications

References 70 publications

Enhancing apoptosis-mediated anticancer activity of evodiamine through protein-based nanoparticles in breast cancer cells

Enhancing apoptosis-mediated anticancer activity of evodiamine through protein-based nanoparticles in breast cancer cells

The development of nanocarriers for natural products

The Interplay between Medical Plants and Gut Microbiota in Cancer

Contact Info

Product

Resources

About