Folate deaminase and cyclic AMP phosphodiesterase in Dictyostelium discoideum: Their regulation by extracellular cyclic AMP and folic acid

Bernstein, R. L.; Rossier, C.; Driel, Roel van; Brunner, Marion; Gerisch, Günther

doi:10.1016/0045-6039(81)90015-4

Cited by 42 publications

(29 citation statements)

References 21 publications

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“…As noted above, stimulation of vegetative cells with folate causes the transient rise of intracellular cGMP [39,43]. There is a degradative enzyme associated with the folate chemotactic system, a folate (or pterin) deaminase (8) that has a similar cellular distribution [44,45] and other properties to those of the PDE [Frazier et al, unpublished]. As expected, a receptor for folate and its analogs is present on the surface of vegetative cells [46,47].…”

Section: Vegetative Chemotaxis Of D Discoideiummentioning

confidence: 74%

Chemotactic Receptors of Dictyostelium discoideum

Frazier

Nandini-Kishore

Meyers

1982

J of Cellular Biochemistry

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For over 30 years, the cellular slime molds, and in particular the species Dictyostelium discoideum, have been viewed as a eukaryotic model system for the study of a variety of regulatory and developmental processes [l]. In particular, the transition from the free-living amoeboid stage to the multicellular slug stage has been the focus of a great deal of attention by those investigators studying the regulation of development [ 1-31. The first morphological evidence that differentiation of a starved population of cells is proceeding on schedule is the appearance of ripples or streams in a lawn of cells resting on a moist surface [l-31. This prelude to the readily identified tight aggregate stage indicates the functioning of the cells' endogenous chemosensory system. In 1969, Bonner and coworkers [4] identified the endogenously produced chemoattractant or acrasin of these cells as cyclic AMP (CAMP). While cAMP is widely encountered as an intracellular regulatory agent or second messenger, the Dictyostelia provide one of the few well documented examples of cAMP as an extracellular messenger molecule.The Polysphondylia such as P violaceum and P pallidum appear to employ a nonnucleotide molecule as their acrasin during starvation induced chemotaxis. This compound has been studied by Bonner and coworkers [S] and has been tentatively identified as a peptide with a blocked amino terminus, although further characterization is required for unambiguous identification. Bonner's group has also been responsible for the identification of folic acid and pterin compounds as chemoattractants of vegetative Dictyostelium amoebae [6-81. This identification of a nonacrasin chemoattractant raises the possibility of studying the transition from a vegetative to a developmental mode of chemotaxis. DEVELOPMENTAL CHEMOTAXIS IN D discoideumThe phenomenology of the CAMP chemotactic system in D discoideum has been well established [9,10]. It is convenient for descriptive purposes to imagine

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Section: Vegetative Chemotaxis Of D Discoideiummentioning

confidence: 74%

Chemotactic Receptors of Dictyostelium discoideum

Frazier

Nandini-Kishore

Meyers

1982

J of Cellular Biochemistry

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“…Thus, induction of phosphodiesterase is either linked to stimulation or to inhibition of development, depending on the temporal pattern of cAMP application. It has been concluded that an adaptation step is implemented in the signal-processing pathway connecting enhancement of development to the activation of cell-surface cAMP receptors, and that adaptation does not shut off the signalprocessing pathway linking phosphodiesterase regulation to the receptors (Bernstein et al, 1981). If HG302 contains a single gene mutation, casAZo00, the gene product affected appears to be a constituent of the proximal part of the signal-processing pathway common to the control of development and of extracellular phosphodiesterase.…”

Section: Discussionmentioning

confidence: 99%

Genetic Analysis of a Dictyostelium discoideum Mutant Resistant to Adenosine 3':5'-Cyclic Phosphorothioate, an Inhibitor of Wild-type Development

Wallraff¹,

Welker²,

Williams³

et al. 1984

Microbiology

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A mutant of Dictyostelium discoideurn strain AX2 with altered responses to cyclic AMP has been analysed genetically. The mutant, HG302, aggregates and fruits in the presence of adenosine 3' : 5'-cyclic phosphorothioate (CAMPS). This slowly hydrolysed analogue of cyclic AMP inhibits wild-type development by blocking cell differentiation from the growth phase to the aggregation-competent stage. The mutant forms small aggregates in the absence and in the presence of CAMPS, and is further distinguished from the wild type by decreased activities of extracellular phosphodiesterase and phosphodiesterase inhibitor. These phenotypic characteristics were all linked to the CAMPS resistance, which was assigned to linkage group 11. Mitotic crossover experiments showed that the CAMPS-resistance mutation, casA1000, maps proximal to the acrA locus in the neighbourhood of whiA. The data suggest that casAlOO0 acts pleiotropically, and that the product of the wild-type allele is required in a part of the signalprocessing pathway common to control of development and regulation of extracellular phosphodiesterase.

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“…Furthermore, amino and amethopterin [2] interact with the same folate receptors [16,17], but are not degraded by the 2-folate deaminase [ 17]. Folic acid, long considered as a mere chemio-attractant, is now suggested as being somewhat interchangeable with the other chemio-attractant cAMP, an important mediator of the aggregation competence, and both extracellular signals could share a common cellular pathway of signal transduction [ 18,19]. In addition, there is a need for a density-dependent 'first messenger' [7,20] acting during the first 3 h of starvation, contrary to the widely accepted view of cAMP being the first messenger.…”

Section: Discussionmentioning

confidence: 99%