Folate content in sea buckthorn berries and related products (Hippophaë rhamnoides L. ssp. rhamnoides): LC-MS/MS determination of folate vitamer stability influenced by processing and storage assessed by stable isotope dilution assay

Abstract

A stable isotope dilution assay for quantitation of folate vitamers in sea buckthorn berries, 27 juice and concentrate using four-fold labeled folate isotopologues of the folate derivatives as 28 the internal standards was adopted using reversed phase liquid chromatography -tandem 29 Sea buckthorn products, Process and storage stability

Folates in Plants: Research Advances and Progress in Crop Biofortification

“…In order to achieve this distinction, chromatographic methods are required. While a number of different detectors such as UV(-DAD), fluorescence, electrochemical and mass spectrometric detectors have been reported in conjunction with high performance liquid chromatography (HPLC) (Pfeiffer et al, 1997; Bagley and Selhub, 2000; Ndaw et al, 2001; Rychlik and Freisleben, 2002; Doherty and Beecher, 2003; Freisleben et al, 2003a; Zhang et al, 2003; Nelson et al, 2004; Fazili et al, 2005; Garratt et al, 2005; Zhang et al, 2005; De Brouwer et al, 2007; Patring and Jastrebova, 2007; Gutzeit et al, 2008), (Ultra-) HPLC coupled to tandem mass spectrometry [(U)HPLC-MS/MS] has become the de facto standard for the sensitive and selective determination of folates (De Brouwer et al, 2010). UHPLC-MS/MS has also been employed for the two-dimensional characterization of folate content in potato tubers (Van Daele et al, 2014).…”

A Novel Hydroxymethyldihydropterin Pyrophosphokinase-dihydropteroate Synthase (HPPK-DHPS) Gene from a Nutraceutical Plant Seabuckthorn, Involved in Folate Pathway is Predominantly Expressed in Fruit Tissue

“…Tracings of tetrahydrofolate were noticed, although distantly lower than the detecting point (Stralsjo et al, 2003). Total folate substances of seabuckthorn fruit berries and juice analyzed ranged from 29 up to 81 μg/100 g. Comparing seabuckthorn folate contents with other berries, reveals that the maximum folate contents of 96 μg/100 g are investigated amongst these berries (Stralsjo et al, 2003;Gutzeit et al, 2008).…”

“…The fruit of seabuckthorn is the store house of vitamins and other important bioactive substances (Xurong et al, 2001). Seabuckthorn berries contain appreciable levels of vitamin B1, P and K (Lu, 1992) and folate vitamers (Gutzeit et al, 2008). Micronutrient deficiency is preventing an estimated one third of the world population from reaching their physical and intellectual potentials.…”

Effects of Processing and Storage on the Stability of Folate Vitamers and Pantothenic Acid in Sea Buckthorn Berries and Related Products (Hippophaë rhamnoidesL. ssp. rhamnoides)

“…This is in contrast to the results of Vahteristo et al (32), who reported that a juice concentrate prepared from a selection of berries and fruits had a folate content below the detection limit. Obviously, the concentration process during thermovacuum (34).…”

“…Deconjugation by treatment with rat plasma and a preparation of chicken pancreas converted polyglutamic vitamers to the respective monoglutamates, which were then detectable by HPLC/ESI-MS-MS. Subsequently sample clean up was performed using strong anion exchange cartridges, which resulted in the HPLC/ESI-MS-MS chromatograms virtually devoid of matrix interferences as displayed in Figure 3 (34).…”