Fodinicurvata sediminis gen. nov., sp. nov. and Fodinicurvata fenggangensis sp. nov., poly- -hydroxybutyrate-producing bacteria in the family Rhodospirillaceae

Abstract

Two Gram-negatively stained, facultatively anaerobic, non-motile, vibrioid and rod-shaped, chemoheterotrophic bacterial strains, designated YIM D82 T and YIM D812 T , were isolated from a salt mine in Yunnan, south-west China. DNA-DNA hybridization, genomic DNA G+C content and phylogenetic analyses based on 16S rRNA gene sequences divided the two isolates into two distinct genospecies that were also clearly differentiated by fatty acid profiles, carbon source utilization patterns, antibiotic susceptibility and biochemical characteristics. The two isolates grew in the presence of 1.5-20 % NaCl, and optimally at 28 6C and pH 7.5. The genomic DNA G+C contents of strains YIM D82 T and YIM D812 T were 61.5 and 62.3 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains YIM D82 T and YIM D812 T were members of the family Rhodospirillaceae and showed 90.5-90.6 % and 90.1-90.2 % similarities with their closest relatives, Rhodovibrio sodomensis and Rhodovibrio salinarum, respectively. Differential phenotypic and genotypic characteristics of the two isolates from recognized genera showed that the two strains should be classified as representing a new genus and two novel species for which the names Fodinicurvata sediminis gen. nov., sp. nov. (type strain YIM D82 T 5DSM 21159 T 5KCTC 22351 T ) and Fodinicurvata fenggangensis sp. nov. (type strain YIM D812 T 5CCTCC AA 208037 T 5DSM 21160 T ) are proposed.

Oleiliquidispirillum nitrogeniifigens gen. nov., sp. nov., a new member of the family Rhodospirillaceae isolated from oil reservoir water

“…Besides, most members of this family were motile by means of a single polar flagellum, such as Tagaea marina [11], Dongia mobilis [12], Pelagibius litoralis [13] and Fodinicurvata halophila [14]. A few members were non-motile, such as Aestuariispira insulae [15] and F. sediminis [16]. In this study, we describe a novel Gram-staining-negative, nonmotile, spiral-shaped bacterium, designated 64-1 T , which was isolated from Liaohe oilfield, north-eastern China, and establish its taxonomic position using a polyphasic approach.…”

Denitrobaculum tricleocarpae gen. nov., sp. nov., a marine bacterium from coralline algae Tricleocarpa sp

“…The Whole Genome Shotgun project of R148 T has been deposited at DDBJ/ENA/GenBank Major cellular fatty acids C 18 : 1 ω7c, C 16 : 1 ω7c, C 19 : 0 cyclo 9, 10 DMA, C 18 : 0 C 18 : 1 ω7c, C 19 : 0 cyclo 9, 10 DMA, C 18 : 0 C 18 : 1 ω7c, C 16 : 1 ω7c, C 19 : 0 cyclo 9, 10 DMA, C 18 : 1 ω7c 10-methyl, C 18 : 0 C 18 : 1 ω7c, C 18 : 1 2-OH, C 16 : 0 C 18 : 1 ω7c, C 16 : 1 ω7c, C 16 : 0 , C 18 : 0 and C 19 : 0 cyclo ω8c *Data from [29] †Data from [24]. ‡Data from [30]. §Data from [31,32].…”

Tagaea marina gen. nov., sp. nov., a marine bacterium isolated from shallow coastal water

“…Other species of the genera Roseospira and Rhodospirillum, such as Roseospira mediosalina, Roseospira navarrensis and Rhodospirillum rubrum, are capable of photoautotrophic growth, together with the above growth patterns (Guyoneaud et al, 2002;Garrity et al, 2005). Obligate chemotrophs in the family Rhodospirillaceae include aerobic and microaerophilic genera such as Azospirillum, Conglomeromonas, Defluviicoccus, Inquilinus, Magnetospirillum, Oceanibaculum, Thalassospira, Tistlia and Tistrella (Tarrand et al, 1978;Skerman et al, 1983;Schleifer et al, 1991;Coenye et al, 2002;Ló pez-Ló pez et al, 2002;Shi et al, 2002;Maszenan et al, 2005;Lai et al, 2009;Díaz-Cárdenas et al, 2010), and facultatively anaerobic genera such as Caenispirillum, Fodinicurvata, Nisaea, Skermanella, Telmatospirillum and Thalassobaculum (Sly & Stackebrandt, 1999;Sizova et al, 2007;Yoon et al, 2007;Zhang et al, 2008;Urios et al, 2008;Wang et al, 2009). All these chemotrophs are heterotrophs but Telmatospirillum siberiense, the only species of Telmatospirillum, also can grow chemolithoautotrophically with hydrogen as an electron donor (Xie & Yokota, 2005).…”