Focusing on bioactive compounds in grapes: stilbenes in Uvalino cv.

Piano, Federico; Bertolone, E.; Pes, D.; Asproudi, Andriani; Borsa, Daniela

doi:10.1007/s00217-013-2060-4

Cited by 8 publications

(3 citation statements)

References 27 publications

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“…Still, we believe it useful to discuss here the main problems related to the (generally) scarce bioavailability of PPs after in vivo administration. Taking resveratrol (and the stilbenes in more general terms) as the paradigmatic PP, its quantity in red wines is usually around 0.6 mg/L [21] but stilbenes can be up to 35 mg/L in certain Piedmont's red wines and autochthonous Uvalino, in particular, contains up to 100 mg/L of resveratrol [48,49]. These figures correspond to concentrations of 2.6, 135, and 438 µM, respectively.…”

Section: Suitability Of the Ex Vivo Approach To Study The Neuroprotecmentioning

confidence: 99%

Protective Effects of Some Grapevine Polyphenols against Naturally Occurring Neuronal Death

et al. 2020

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The interest in the biological properties of grapevine polyphenols (PPs) in neuroprotection is continuously growing in the hope of finding translational applications. However, there are several concerns about the specificity of action of these molecules that appear to act non-specifically on the permeability of cellular membranes. Naturally occurring neuronal death (NOND) during cerebellar maturation is a well characterized postnatal event that is very useful to investigate the death and rescue of neurons. We here aimed to establish a baseline comparative study of the potential to counteract NOND of certain grapevine PPs of interest for the oenology. To do so, we tested ex vivo the neuroprotective activity of peonidin- and malvidin-3-O-glucosides, resveratrol, polydatin, quercetin-3-O-glucoside, (+)-taxifolin, and (+)-catechin. The addition of these molecules (50 μM) to organotypic cultures of mouse cerebellum explanted at postnatal day 7, when NOND reaches a physiological peak, resulted in statistically significant (two-tailed Mann–Whitney test—p < 0.001) reductions of the density of dead cells (propidium iodide+ cells/mm2) except for malvidin-3-O-glucoside. The stilbenes were less effective in reducing cell death (to 51–60%) in comparison to flavanols, (+)-taxifolin and quercetin 3-O-glucoside (to 69–72%). Thus, molecules with a -OH group in ortho position (taxifolin, quercetin 3-O-glucoside, (+)-catechin, and peonidin 3-O-glucoside) have a higher capability to limit death of cerebellar neurons. As NOND is apoptotic, we speculate that PPs act by inhibiting executioner caspase 3.

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Section: Suitability Of the Ex Vivo Approach To Study The Neuroprotecmentioning

confidence: 99%

Protective Effects of Some Grapevine Polyphenols against Naturally Occurring Neuronal Death

et al. 2020

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“…Bioassay-guided fractionation of the ethyl acetate extract from M. quinquenervia fruits resulted in the isolation of two new flavanones ( 1a and 1b ), one new chalcone ( 3 ), along with 16 known compounds including (2 S ,11 R ) melanervin ( 2a ), , (2 R ,11 S ) melanervin ( 2b ), , pinocembrin ( 4 ), strobopinin ( 5 ), cryptostrobin ( 6 ), cis -piceatannol ( 7 ), piceatannol ( 8 ), (1,1′-biphenyl)-2,2′,4,4′,5-pentol,3,3′,5′-trimethoxy ( 9 ), 3,4,3′-tri- O -methylellagic acid ( 10 ), 3-methoxy-5-methyl-1,2-benzenediol ( 11 ), 2-methoxy-1,3,5-benzenetriol ( 12 ), lupenone ( 13 ), methyl betulinate ( 14 ), 3- O -acetylbetulinic acid ( 15 ), pyracrenic acid ( 16 ), and oleanolic acid acetate ( 17 ) . The structures were determined by analyzing spectroscopic data such as UV, NMR, infrared (IR), and MS and comparing them to literature values.…”

Section: Resultsmentioning

confidence: 99%

Bioassay-Guided Isolation and Identification of Cytotoxic Compounds from Melaleuca quinquenervia Fruits

Raksat,

Atanu,

Mendez

et al. 2024

ACS Omega

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The fruit extract of Melaleuca quinquenervia yielded a total of 19 compounds, including two novel spiro-biflavonoid enantiomers (1a and 1b) and a chalcone derivative (3). Their structures were determined through spectroscopic analysis. The enantiomers of the racemic mixture of compound 1 were successfully resolved into (+)-1 and (−)-1 using chiral-phase HPLC. Single-crystal X-ray diffraction analysis was also used to confirm the structure of 1. The enantiomeric configurations of 1 and 2 were determined through a comparison of the calculated and experimental electronic circular dichroism spectra. Compounds 2 (melanervin), 14 (methyl betulinate), 15 (3-O-acetylbetulinic acid), and 16 (pyracrenic acid) were found to be highly cytotoxic, with compound 16 showing superior growth inhibition of nonsmall cell lung cancer cells (A549 cells) (IC 50 2.8 ± 0.1 μM) compared to cisplatin (IC 50 3.3 ± 0.0 μM), a positive control chemotherapeutic drug. Both compound 16 and cisplatin were significantly more cytotoxic toward A549 lung cancer cells compared to nontumorigenic Vero E6 cells.

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“…As can be observed from Table 1 , the concentration of active compounds from grapevine wastes strongly depends on several factors, including, but not limited to, the used variety, harvesting time, and extraction method applied. Furthermore, applying a post-harvest treatment in the form of wilting can considerably increase the contents of anthocyanins, proanthocyanidins and stilbenes in the grape composition [ 33 ]. Rätsep et al [ 27 ] have conducted a study evaluating the polyphenolic content of grapevines and canes in accordance with the pruning time and dormancy phase of the grapevine.…”

Section: Extraction Methods Of Bioactive Compounds From Grapevine Wastementioning

confidence: 99%

Grapevine Wastes: A Rich Source of Antioxidants and Other Biologically Active Compounds

et al. 2022

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Wine production is one of the most critical agro-industrial sectors worldwide, generating large amounts of waste with negative environmental impacts, but also with high economic value and several potential applications. From wine shoots to grape pomace or seeds, all of the wastes are rich sources of bioactive compounds with beneficial effects for human health, with these compounds being raw materials for other industries such as the pharmaceutical, cosmetic or food industries. Furthermore, these compounds present health benefits such as being antioxidants, supporting the immune system, anti-tumoral, or preventing cardiovascular and neural diseases. The present work aims to be a critical discussion of the extraction methods used for bioactive compounds from grapevine waste and their beneficial effects on human health.

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Focusing on bioactive compounds in grapes: stilbenes in Uvalino cv.

Cited by 8 publications

References 27 publications

Protective Effects of Some Grapevine Polyphenols against Naturally Occurring Neuronal Death

Protective Effects of Some Grapevine Polyphenols against Naturally Occurring Neuronal Death

Bioassay-Guided Isolation and Identification of Cytotoxic Compounds from Melaleuca quinquenervia Fruits

Grapevine Wastes: A Rich Source of Antioxidants and Other Biologically Active Compounds

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