Focused ultrasound treatment of methicilin resistant Staphylococcus aureus induced abscesses: pre-clinical study

Curiel, Laura; Mougenot, Charles; Rieck, Birgit; Zhang, Kunyan; Bates, D. Gregory; Pichardo, Samuel

doi:10.1186/2050-5736-3-s1-p58

Cited by 2 publications

(2 citation statements)

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“…Although few studies have investigated the treatment of infectious diseases with HIFU, these studies have yielded promising results. For example, HIFU exhibits bactericidal activity against some bacterial abscesses, including Enterococcus faecalis, E. coli, and S. aureus [23][24][25]34]. Here, we hypothesized that HIFU might inhibit MTB growth as the basis of a noninvasive TB treatment.…”

Section: Discussionmentioning

confidence: 99%

“…This accumulation of energy typically heats the targeted tissue, possibly leading to thermally-induced cell death and/or coagulation necrosis [16]. HIFU has been tested as a treatment for deep bacterial infections (e.g., Staphylococcus aureus) in a rat model because of its capability to induce a rise of temperature at a very precise location [23][24][25][26]. Some studies have also demonstrated that cavitation-based HIFU histotripsy has shown potential for treating common bacterial pathogens, including Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

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Bactericidal effects of high intensity focused ultrasound on Bacillus Calmette–Guerin in vivo and in vitro

Xie

Cao

Jian-hu

et al. 2019

International Journal of Hyperthermia

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Purpose: The objective of this study was to investigate the bactericidal effects of high intensity focused ultrasound (HIFU) on Bacillus Calmette-Guerin (BCG, a substitute for Mycobacterium tuberculosis) in vitro and in vivo, and to explore the underlying mechanisms. Materials and methods: HIFU, at a fixed frequency of 1 MHz, was applied to both BCG culture suspensions and subcutaneous BCG abscesses in rats. Results: HIFU irradiation significantly reduced the bacterial survival rate and caused temperature elevations both in vitro and in vivo. Furthermore, BCG suspensions irradiated for 15 s at 3185 and 6369 W/cm 2 had increased cell wall damage, which resulted in morphological changes compared to the untreated control group. Additionally, we observed histological changes in the rat subcutaneous abscesses after HIFU ablation at 6369 W/cm 2 . H&E staining of infected lesions showed coagulative necrosis with central nucleus dissolution and increased infiltration of inflammatory cells, as well as nuclear pyknosis and nuclear fragmentation in the periphery. The volumes of the subcutaneous abscesses in the HIFU-treated group were significantly lower than those in the sham-treated group. Conclusion: HIFU has the therapeutic potential to treat BCG-infected tissues in rats. We theorize that a combination of mechanical, cavitation, and thermal effects most efficiently inactivate BCG bacteria via HIFU. This study is expected to provide a bio-plausible basis for a noninvasive and effective treatment for tuberculosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bactericidal effects of high intensity focused ultrasound on Bacillus Calmette–Guerin in vivo and in vitro

Xie

Cao

Jian-hu

et al. 2019

International Journal of Hyperthermia

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Inactivation of Planktonic Escherichia coli by Focused 2-MHz Ultrasound

Brayman

MacConaghy

Wang

et al. 2017

Ultrasound in Medicine & Biology

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This study was motivated by the desire to develop a noninvasive means to treat abscesses, and represents first steps toward that goal. Non-thermal, high intensity focused ultrasound (HIFU) was used to inactivate Escherichia coli (~1 × 109 cells/mL) in suspension. Cells were treated in 96-well culture plate wells using 1.95 MHz ultrasound and incident focal acoustic pressures as high as 16 MPa peak positive and 9.9 MPa peak negative (free field measurements). Surviving fraction was assessed by coliform culture, and by alamarBlue® assay. There was no biologically significant heating associated with ultrasound exposure. Bacterial inactivation kinetics were well described by a half-life model, with a half-time of 1.2 min. At the highest exposure levels, a two-log inactivation was typically achieved within ten minutes. The free field-equivalent peak negative acoustic pressure threshold for inactivation was ~7 MPa. At the highest acoustic pressures used, inactivation efficacy was insensitive to reciprocal changes in pulse length and pulse repetition frequency at constant duty factor. Although treated volumes were very small, proof of principal was provided by these experiments.

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Focused ultrasound treatment of methicilin resistant Staphylococcus aureus induced abscesses: pre-clinical study

Cited by 2 publications

References 0 publications

Bactericidal effects of high intensity focused ultrasound on Bacillus Calmette–Guerin in vivo and in vitro

Bactericidal effects of high intensity focused ultrasound on Bacillus Calmette–Guerin in vivo and in vitro

Inactivation of Planktonic Escherichia coli by Focused 2-MHz Ultrasound

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