2011
DOI: 10.1016/j.carres.2010.08.016
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Focused directed evolution of β-glucosidases: theoretical versus real effectiveness of a minimal working setup and simple robust screening

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Cited by 3 publications
(2 citation statements)
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“…Positive clones were screened for activity directly on Petri dishes by a procedure developed to identify clones with β-glucosidase activity using the chromogenic substrate 5-bromo-4-chloro-3-indolyl-O-β-D-glucopyranoside (XGlc, supplied by Biosynth AG, Staad, Switzerland), which yields a blue reaction product [2]. A 40 mg L −1 dose of XGlc was found to be sufficient and cultivation at 37°C for 12 h, followed by 12 h at 16°C, gave the best results.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Positive clones were screened for activity directly on Petri dishes by a procedure developed to identify clones with β-glucosidase activity using the chromogenic substrate 5-bromo-4-chloro-3-indolyl-O-β-D-glucopyranoside (XGlc, supplied by Biosynth AG, Staad, Switzerland), which yields a blue reaction product [2]. A 40 mg L −1 dose of XGlc was found to be sufficient and cultivation at 37°C for 12 h, followed by 12 h at 16°C, gave the best results.…”
Section: Methodsmentioning
confidence: 99%
“…Thus, ideally parameters describing the performance of mutagenesis methods should be empirically estimated and incorporated into the formulas to avoid underestimation of required screening efforts [2]. The screening is usually the most expensive part of the overall effort, so it is highly desirable to identify the most appropriate screening scale.…”
Section: Introductionmentioning
confidence: 99%