2016
DOI: 10.1109/tcbb.2015.2459685
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FocusALL: Focal Stacking of Microscopic Images Using Modified Harris Corner Response Measure

Abstract: Automated image analysis of microscopic images such as protein crystallization images and cellular images is one of the important research areas. If objects in a scene appear at different depths with respect to the camera's focal point, objects outside the depth of field usually appear blurred. Therefore, scientists capture a collection of images with different depths of field. Focal stacking is a technique of creating a single focused image from a stack of images collected with different depths of field. In t… Show more

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Cited by 31 publications
(13 citation statements)
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“…TDOA is a technology derived from TOA. Its main advantage is to overcome the problem of electronic clock time synchronization, that is, it does not require a strictly synchronized electronic clock [21,22]. Assuming there are two signals with different propagation speeds, the calculation formula of distance R is as follows:…”
Section: Fusion Modelmentioning
confidence: 99%
“…TDOA is a technology derived from TOA. Its main advantage is to overcome the problem of electronic clock time synchronization, that is, it does not require a strictly synchronized electronic clock [21,22]. Assuming there are two signals with different propagation speeds, the calculation formula of distance R is as follows:…”
Section: Fusion Modelmentioning
confidence: 99%
“…Particularly, all these approaches can be classified into three groups: spatial domain (based on pixel value), transform domain (based on frequency components), and deep learning methods. Spatial domain image fusion methods select pixels or regions from the focused parts in the spatial domain to compose the final image [ 10 , 11 ]. Different transform domain methods are used for image resolution enhancement through the transformation of the source image into different scales, which then are composed into one fused image [ 12 ].…”
Section: Related Workmentioning
confidence: 99%
“…All these methods can be used to obtain the best focus image from a set of captured microscopic images, but the performance time is the essential factor for the embryo selection task. Speed of microscopic image processing by focus stacking is highlighted by many researchers [ 11 , 20 ], but not less relevant are image spectral information, features to be retrieved or similarity based metrics. For our problem, the latter metrics are important as far as it can affect the correct embryo identification regarding the number of cells.…”
Section: Related Workmentioning
confidence: 99%
“…Usually, crystal images are expected to have distinguishable features such as high intensity, sharp clear edges, and proper geometric shapes. However, in some cases, these features may not be dominant due to focusing or reflection problems even if there is a protein crystal in the image [4]. Therefore, a single type of thresholding technique may not provide an informative binary image for classifying images.…”
Section: Introductionmentioning
confidence: 99%