Focus Issue on Metabolism: Metabolites, Metabolites Everywhere

Fernie, Alisdair R.; Pichersky, Eran

doi:10.1104/pp.15.01499

Cited by 35 publications

(26 citation statements)

References 39 publications

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“…In recent years, the leap of genomic technologies, with the relative ease in collecting large-scale sequence data, has bred new life into metabolism research [26]. The increasing number of available genome sequence is now frequently integrated with high-resolution/deep-coverage metabolomics approaches [27] not only to uncover structural and regulatory aspects of pathways of secondary metabolism, but also to go deeper into the evolution of metabolism across the diversification of land plants (and landmark examples in this area are the recent reconstructions of the synthesis of nicotine and caffeine [28,29]). The case studies presented here thus represent successful examples of how targeted molecular approaches or, more recently, the combination of next-generation genomics with metabolic profiling are revolutionizing the field of medicinal plants with new knowledge concerning the synthesis of natural products.…”

Section: Approaches For Pathway Discoverymentioning

confidence: 99%

The Integration of Metabolomics and Next-Generation Sequencing Data to Elucidate the Pathways of Natural Product Metabolism in Medicinal Plants

et al. 2018

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Plants have always been used as medicines since ancient times to treat diseases. The knowledge around the active components of herbal preparations has remained nevertheless fragmentary: the biosynthetic pathways of many secondary metabolites of pharmacological importance have been clarified only in a few species, while the chemodiversity present in many medicinal plants has remained largely unexplored. Despite the advancements of synthetic biology for production of medicinal compounds in heterologous hosts, the native plant species are often the most reliable and economic source for their production. It thus becomes fundamental to investigate the metabolic composition of medicinal plants to characterize their natural metabolic diversity and to define the biosynthetic routes of important compounds to develop strategies to further increase their content. We present here a number of case studies for selected classes of secondary metabolites and we review their health benefits and the historical developments in their structural elucidation and characterization of biosynthetic genes. We cover the cases of benzoisoquinoline and monoterpenoid indole alkaloids, cannabinoids, caffeine, ginsenosides, withanolides, artemisinin, and taxol; we show how the "early" biochemical or the more recent integrative approaches-based on omics-analyses-have helped to elucidate their metabolic pathways and cellular compartmentation. We also summarize how the knowledge generated about their biosynthesis has been used to develop metabolic engineering strategies in heterologous and native hosts. We conclude that following the advent of novel, high-throughput and cost-effective analytical technologies, the secondary metabolism of medicinal plants can now be examined under the lens of systems biology.

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Section: Approaches For Pathway Discoverymentioning

confidence: 99%

The Integration of Metabolomics and Next-Generation Sequencing Data to Elucidate the Pathways of Natural Product Metabolism in Medicinal Plants

et al. 2018

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“…Low molecular weight organic compounds are commonly separated by perspective function into primary metabolites, secondary metabolites (also called specialized metabolites or natural products), and plant hormones ( Figure 1) (Taiz et al, 2015). Primary metabolites are highly conserved and directly required for the growth and development of plants (Fernie and Pichersky, 2015). Secondary metabolites, including major groups such as phenolics, terpenes, and nitrogen-containing compounds, are often lineage-specific and aid plants to interact with the biotic and abiotic environment (Hartmann, 2007).…”

Section: Introductionmentioning

confidence: 99%

Plant Secondary Metabolites as Defenses, Regulators, and Primary Metabolites: The Blurred Functional Trichotomy

2020

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The plant kingdom produces hundreds of thousands of low molecular weight organic compounds. Based on the assumed functions of these compounds, the research community has classified them into three overarching groups: primary metabolites, which are directly required for plant growth; secondary (or specialized) metabolites, which mediate plant-environment interactions; and hormones, which regulate organismal processesand metabolism. For decades, this functional trichotomy of plant metabolism has shaped theory and experimentation in plant biology. However, exact biochemical boundaries between these different metabolite classes were never fully established. A new wave of genetic and chemical studies now further blurs these boundaries by demonstrating that secondary metabolites are multifunctional; they can function as potent regulators of plant growth and defense as well as primary metabolites sensu lato. Several adaptive scenarios may have favored this functional diversity for secondary metabolites, including signaling robustness and cost-effective storage and recycling. Secondary metabolite multifunctionality can provide new explanations for ontogenetic patterns of defense production and can refine our understanding of plant-herbivore interactions, in particular by accounting for the

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“…Metabolites are defined as compounds produced by plants to perform principal functions, such as growth and development (primary metabolites), and specialized functions, such as defense against most of the attackers herbivores and pathogens (secondary metabolites) (Hartmann, 2007;Fernie & Pichersky, 2015;Pagare et al, 2015;Erb & Kliebenstein, 2020). It is impossible for any analysis to reflect the whole metabolome of a specific tissue, due to its great chemical diversity (Weckwerth, 2003;Kopka et al, 2004;Lu et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Metabolomic Profiling and Antioxidant Activity of Trigonella foenum-graecum and Trigonella hamosa

2021

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Egyptian Journal of Botany http://ejbo.journals.ekb.eg/ 39 T HE METABOLOMIC profiling of Trigonella foenum-graecum and Trigonella hamosa seeds was performed using NMR spectroscopy combined with multivariate data analysis (MVDA). The database of the chemical shifts and the J-resolved two-dimensional NMR technique were used to identify the metabolites. Additionally, the identified metabolites were confirmed by comparison with previously identified compounds as well. Among the elucidated metabolites, choline, sterols, leucine, valine, flavonoids derivatives and trigonelline were found to contribute to distinguish between the two species of Trigonella in score scatter and loading plots of multivariate data analysis. Phytochemical analysis of plant seeds extracts for total carbohydrates, proteins, phenolics, flavonoids, saponins and anthocyanins was performed by spectrophotometer. The antioxidant potential of both species was evaluated by phosphomolybdenum method and DPPH radical scavenging activity. T. foenum-graecum was characterized by higher concentrations of trigonelline, sterols, protein, flavonoids derivatives, saponins and anthocyanins, and lower IC 50 in DPPH radical scavenging activity assay than T. hamosa. It was observed that total antioxidant capacity was positively correlated with glucose, trigonelline, protein, flavonoids, saponins and anthocyanins. T. foenum-graecum revealed an interesting metabolomic pattern with antioxidant activity that assigns this plant as a promising candidate to explore its targeted and non-targeted metabolomics profile along with potential bioactivities.

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Focus Issue on Metabolism: Metabolites, Metabolites Everywhere

Cited by 35 publications

References 39 publications

The Integration of Metabolomics and Next-Generation Sequencing Data to Elucidate the Pathways of Natural Product Metabolism in Medicinal Plants

The Integration of Metabolomics and Next-Generation Sequencing Data to Elucidate the Pathways of Natural Product Metabolism in Medicinal Plants

Plant Secondary Metabolites as Defenses, Regulators, and Primary Metabolites: The Blurred Functional Trichotomy

Metabolomic Profiling and Antioxidant Activity of Trigonella foenum-graecum and Trigonella hamosa

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