Focalization Performance Study of a Novel Bulk Acoustic Wave Device

Barbaresco, Federica; Racca, Luisa; Spigarelli, Luca; Cocuzza, Matteo; Pirri, Candido Fabrizio; Canavese, Giancarlo

doi:10.3390/nano11102630

Cited by 2 publications

(2 citation statements)

References 56 publications

(82 reference statements)

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“…Acoustic separation technology is primarily categorized into two types: surface acoustic waves (SAWs) [25,26] and bulk acoustic waves (BAWs) [27,28]. SAW devices (operating at frequencies of 1-1000 MHz [20]) generate a high-intensity acoustic field and utilize the powerful force of acoustic radiation to promote the migration of cancer cells toward the acoustic nodes, thus enabling the isolation of cancer cells [29,30].…”

Section: Introductionmentioning

confidence: 99%

The Shape Effect of Acoustic Micropillar Array Chips in Flexible Label-Free Separation of Cancer Cells

Lin,

Zhu,

et al. 2024

Micromachines

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The precise isolation of circulating tumor cells (CTCs) from blood samples is a potent tool for cancer diagnosis and clinical prognosis. However, CTCs are present in extremely low quantities in the bloodstream, posing a significant challenge to their isolation. In this study, we propose a non-contact acoustic micropillar array (AMPA) chip based on acoustic streaming for the flexible, label-free capture of cancer cells. Three shapes of micropillar array chips (circular, rhombus, and square) were fabricated. The acoustic streaming characteristics generated by the vibration of microstructures of different shapes are studied in depth by combining simulation and experiment. The critical parameters (voltage and flow rate) of the device were systematically investigated using microparticle experiments to optimize capture performance. Subsequently, the capture efficiencies of the three micropillar structures were experimentally evaluated using mouse whole blood samples containing cancer cells. The experimental results revealed that the rhombus microstructure was selected as the optimal shape, demonstrating high capture efficiency (93%) and cell activity (96%). Moreover, the reversibility of the acoustic streaming was harnessed for the flexible release and capture of cancer cells, facilitating optical detection and analysis. This work holds promise for applications in monitoring cancer metastasis, bio-detection, and beyond.

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Section: Introductionmentioning

confidence: 99%

The Shape Effect of Acoustic Micropillar Array Chips in Flexible Label-Free Separation of Cancer Cells

Lin,

Zhu,

et al. 2024

Micromachines

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“…Microfluidic devices for measuring cell concentration use various principles and techniques to accurately quantify the cell concentration in a given sample [18], such as flow cytometry [19,20], impedance spectroscopy [21][22][23], digital microfluidics [24][25][26][27][28][29], acousticbased microfluidic devices [30,31], andmicroscopy and image analysis [32,33]. These techniques are designed to be highly sensitive and efficient, making them helpful tools in fields such as biology, medicine, and biotechnology.…”

Section: Introductionmentioning

confidence: 99%

Projection Micro-Stereolithography to Manufacture a Biocompatible Micro-Optofluidic Device for Cell Concentration Monitoring

Saitta,

Cutuli,

Celano

et al. 2023

Polymers

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In this work, a 3D printed biocompatible micro-optofluidic (MoF) device for two-phase flow monitoring is presented. Both an air–water bi-phase flow and a two-phase mixture composed of micrometric cells suspended on a liquid solution were successfully controlled and monitored through its use. To manufacture the MoF device, a highly innovative microprecision 3D printing technique was used named Projection Microstereolithography (PμSL) in combination with the use of a novel 3D printable photocurable resin suitable for biological and biomedical applications. The concentration monitoring of biological fluids relies on the absorption phenomenon. More precisely, the nature of the transmission of the light strictly depends on the cell concentration: the higher the cell concentration, the lower the optical acquired signal. To achieve this, the microfluidic T-junction device was designed with two micrometric slots for the optical fibers’ insertion, needed to acquire the light signal. In fact, both the micro-optical and the microfluidic components were integrated within the developed device. To assess the suitability of the selected biocompatible transparent resin for optical detection relying on the selected working principle (absorption phenomenon), a comparison between a two-phase flow process detected inside a previously fully characterized micro-optofluidic device made of a nonbiocompatible high-performance resin (HTL resin) and the same made of the biocompatible one (BIO resin) was carried out. In this way, it was possible to highlight the main differences between the two different resin grades, which were further justified with proper chemical analysis of the used resins and their hydrophilic/hydrophobic nature via static water contact angle measurements. A wide experimental campaign was performed for the biocompatible device manufactured through the PμSL technique in different operative conditions, i.e., different concentrations of eukaryotic yeast cells of Saccharomyces cerevisiae (with a diameter of 5 μm) suspended on a PBS (phosphate-buffered saline) solution. The performed analyses revealed that the selected photocurable transparent biocompatible resin for the manufactured device can be used for cell concentration monitoring by using ad hoc 3D printed micro-optofluidic devices. In fact, by means of an optical detection system and using the optimized operating conditions, i.e., the optimal values of the flow rate FR=0.1 mL/min and laser input power P∈{1,3} mW, we were able to discriminate between biological fluids with different concentrations of suspended cells with a robust working ability R2=0.9874 and Radj2=0.9811.

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Focalization Performance Study of a Novel Bulk Acoustic Wave Device

Cited by 2 publications

References 56 publications

The Shape Effect of Acoustic Micropillar Array Chips in Flexible Label-Free Separation of Cancer Cells

The Shape Effect of Acoustic Micropillar Array Chips in Flexible Label-Free Separation of Cancer Cells

Projection Micro-Stereolithography to Manufacture a Biocompatible Micro-Optofluidic Device for Cell Concentration Monitoring

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