Focal Cerebral Ischaemia in the Rat: 2. Regional Cerebral Blood Flow Determined by [¹⁴C]Iodoantipyrine Autoradiography following Middle Cerebral Artery Occlusion

Abstract

Summary: Local cerebral blood flow has been measured by quantitative autoradiography, employing ['4Cjiodoantipyrine as tracer, in rats killed half an hour after occlusion of the middle cerebral artery. The results were compared with pattern of local cerebral blood flow (CBF) in sham-operated rats and with neuropathological findings. In every animal there was a profound reduction (to 13% of control levels) in blood flow in the neocortex previously supplied by the occluded artery. The level of blood flow in the areas in which ischaemic brain damage occurred was 0.24 ±0.03 ml g min (mean ± SEM). This level of CBF is considerably greater than that reported following a similar surgical procedure in cats and primates. Moderate reductions in blood flow were also seen outside the territory of the occluded artery and in parts of the opposite hemisphere. Absolute increases in blood flow (hyperaemia) were seen only in the substantia nigra and globus pallidus ipsilateral to the occlusion. It is suggested that this finding and the reductions in blood flow outside the territory of the middle cerebral artery are reflections of alterations in neuronal function and metabolic activity secondary to the ischaemic lesion. Key Words: Cerebrovascular circulationCerebral ischaemiaRat.In the past five years a range of reliable quantitative techniques have become available which permit accurate assessment of various neurochemical and cerebral circulatory parameters in discrete anatomical regions within the central nervous system.

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“…Decreases in contralateral perfusion have been described in previous CBF evaluations during and after focal ischemia in naïve animals (Tamura et al, 1981;Zhao et al, 1997), and were also evident in both sham and PC rats in a previous PC study (Chen et al, 1996). The absolute contralateral blood flow measured in the naïve group at 3 h is identical to that obtained at 2.5 h in a separate study from this laboratory (Kamiya et al, 2005).…”

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“…The distal MCA was electrocoagulated and cut with a technique [27] modified for mouse from Tamamura [28,29]. This procedure causes a large infarct involving cortical and subcortical zones [30].…”

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