Focal adhesion signaling pathway involved in skin immune response of tongue sole Cynoglossus semilaevis to Vibrio vulnificus infection

Liu, Houfu; Hu, Xiucai; Lian, Zhengyi; Luo, Zhengrong; Lv, Aijun; Tan, Jing

doi:10.1016/j.fsi.2023.108651

Cited by 10 publications

(3 citation statements)

References 43 publications

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“…Elevation in the level of Collagen alpha-3(VI) chain has been reported in serum of patients with gastrointestinal disorders (25). It was also found upregulated in intestine tissue of tongue sole Cynoglossus semilaevis after Vibrio vulnificus infection (26). This might be an indication that Ah infection mediates changes in ECM composition that causes matrix rigidity.…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of gut inLabeo rohitareveals ECM as Key Player in host’s Response toAeromonas hydrophilaInfection

Nissa,

Pinto,

Ghosh

et al. 2024

Preprint

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In the aquaculture sector, one of the challenges include disease outbreaks such as bacterial infections, particularly fromAeromonas hydrophila(Ah), impacting both wild and farmed fish. In this study, we conducted a proteomic analysis of the gut tissue inLabeo rohitafollowingAhinfection to elucidate the protein alterations and its implications for immune response. Our findings reveal significant dysregulation in extracellular matrix (ECM) associated proteins duringAhinfection, with increased abundance of elastin and Collagen alpha-3(VI) contributing to matrix rigidity. Pathway and enrichment analysis of differentially expressed proteins (DEPs) highlights the involvement of ECM-related pathways, including Focal adhesions, Integrin cell surface interactions, and actin cytoskeleton organization.Focal adhesions, crucial for connecting intracellular actin bundles to the ECM, play a pivotal role in immune response during infections. Increased abundance of integrin alpha 1, integrin beta 1, and Tetraspanin suggests their involvement in the host’s response toAhinfection. Proteins associated with actin cytoskeleton reorganization, such as myosin, tropomyosin, and phosphoglucomutase, exhibit increased abundance, influencing changes in cell behavior. Additionally, upregulated proteins like LTBP1 and Fibrillin-2 contribute to TGF-β signaling and focal adhesion, indicating their role in immune regulation.The study also identifies elevated levels of laminin, galectin 3, and tenascin-C, which interact with integrins and other ECM components, influencing immune cell migration and function. These proteins, along with decorin and lumican, act as immunomodulators, coordinating pro- and anti-inflammatory responses. ECM fragments released during pathogen invasion serve as “danger signals,” initiating pathogen clearance and tissue repair through Toll-like receptor signaling.IMPORTANCEThe study underscores the critical role of the extracellular matrix (ECM) and its associated proteins in the immune response of aquatic organisms during bacterial infections likeAeromonas hydrophila(Ah). Understanding the intricate interplay between ECM alterations and immune response pathways provides crucial insights for developing effective disease control strategies in aquaculture. By identifying key proteins and pathways involved in host defense mechanisms, this research lays the groundwork for targeted interventions to mitigate the impact of bacterial infections on fish health and aquaculture production.

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Section: Discussionmentioning

confidence: 99%

Proteomic analysis of gut inLabeo rohitareveals ECM as Key Player in host’s Response toAeromonas hydrophilaInfection

Nissa,

Pinto,

Ghosh

et al. 2024

Preprint

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“…Focal adhesion (FA) is critical for cell adhesion, migration, and antibacterial immunity ( 105 ). The FA signalling pathway not only mediates cell attachment to the ECM and growth substratum, but it also regulates different developmental and pathological processes, making it a transitory structure that allows cells to interact with their environment.…”

Section: Discussionmentioning

confidence: 99%

Morphologic, cytometric, quantitative transcriptomic and functional characterisation provide insights into the haemocyte immune responses of Pacific abalone (Haliotis discus hannai)

Ma,

Wu,

Zhang

et al. 2024

Front. Immunol.

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In recent years, the abalone aquaculture industry has been threatened by the bacterial pathogens. The immune responses mechanisms underlying the phagocytosis of haemocytes remain unclear in Haliotis discus hannai. It is necessary to investigate the immune mechanism in response to these bacterial pathogens challenges. In this study, the phagocytic activities of haemocytes in H. discus hannai were examined by flow cytometry combined with electron microscopy and transcriptomic analyses. The results of Vibrio parahaemolyticus, Vibrio alginolyticus and Staphylococcus aureu challenge using electron microscopy showed a process during phagosome formation in haemocytes. The phagocytic rate (PP) of S. aureus was higher than the other five foreign particles, which was about 63%. The PP of Vibrio harveyi was about 43%, the PP peak of V. alginolyticus in haemocyte was 63.7% at 1.5 h. After V. parahaemolyticus and V. alginolyticus challenge, acid phosphatase, alkaline phosphatase, total superoxide dismutase, lysozyme, total antioxidant capacity, catalase, nitric oxide synthase and glutathione peroxidase activities in haemocytes were measured at different times, differentially expressed genes (DEGs) were identified by quantitative transcriptomic analysis. The identified DEGs after V. parahaemolyticus challenge included haemagglutinin/amebocyte aggregation factor-like, supervillin-like isoform X4, calmodulin-like and kyphoscoliosis peptidase-like; the identified DEGs after V. alginolyticus challenge included interleukin-6 receptor subunit beta-like, protein turtle homolog B-like, rho GTPase-activating protein 6-like isoform X2, leukocyte surface antigen CD53-like, calponin-1-like, calmodulin-like, troponin C, troponin I-like isoform X4, troponin T-like isoform X18, tumor necrosis factor ligand superfamily member 10-like, rho-related protein racA-like and haemagglutinin/amebocyte aggregation factor-like. Some immune-related KEGG pathways were significantly up-regulated or down-regulated after challenge, including thyroid hormone synthesis, Th17 cell differentiation signalling pathway, focal adhesion, melanogenesis, leukocyte transendothelial migration, inflammatory mediator regulation of TRP channels, ras signalling pathway, rap1 signalling pathway. This study is the first step towards understanding the H. discus hannai immune system by adapting several tools to gastropods and providing a first detailed morpho-functional study of their haemocytes.

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“…Focal adhesions can also transmit signals that affect cell survival or differentiation by altering gene expression ( Legate, et al, 2009 ). The focal adhesion signaling pathway plays a pivotal role in cell adhesion, migration and antibacterial immunity ( Liu, et al, 2023 ). Ablation of the adhesion protein PINCH-1 (also known as Lims1) gene in the mouse epidermis had been observed to result in detachment of the epidermal basement membrane, excessive epidermal thickening, and progressive hair loss ( Karaköse, et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of key modules and hub genes involved in regulating the feather follicle development of Wannan chickens using WGCNA

Wang,

Xing,

Wang

et al. 2024

Poultry Science

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Focal adhesion signaling pathway involved in skin immune response of tongue sole Cynoglossus semilaevis to Vibrio vulnificus infection

Cited by 10 publications

References 43 publications

Proteomic analysis of gut inLabeo rohitareveals ECM as Key Player in host’s Response toAeromonas hydrophilaInfection

Proteomic analysis of gut inLabeo rohitareveals ECM as Key Player in host’s Response toAeromonas hydrophilaInfection

Morphologic, cytometric, quantitative transcriptomic and functional characterisation provide insights into the haemocyte immune responses of Pacific abalone (Haliotis discus hannai)

Identification of key modules and hub genes involved in regulating the feather follicle development of Wannan chickens using WGCNA

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