Foaming and Emulsifying Properties of Porcine Red Cell Protein Concentrate

Salvador, P.; Saguer, E.; Parés, Dolors; Carrétéro, C.; Toldrà, Mònica

doi:10.1177/1082013209353223

Cited by 8 publications

(4 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, temperature >60 °C is not recommended. Rustom et al. (1991) also reported that the increase in temperature from 20 to 60 °C would decrease the extracted protein.…”

Section: Resultsmentioning

confidence: 95%

Optimization of hexametaphosphate-assisted extraction and functional characterization of palm kernel cake protein

Chee

Ayob

2013

Food sci. technol. int.

View full text Add to dashboard Cite

Response surface methodology was applied to study the optimization of palm kernel cake protein (PKCP) hexametaphosphate-assisted extraction. The optimum PKCP yield (28.37%) when extracted using 1.50% sodium hexametaphosphate (SHMP) of pH 10, at 50 °C, and the 1:70 (w/v) ratio of cake-to-solvent was significantly (P < 0.05) higher than the protein yield from an alkaline (pH 10) extraction (8.12 ± 0.24%). Differential scanning calorimetry (DSC) analysis showed a higher denatured temperature (99.78 °C) for PKCP as compared with alkaline extracted one (96.96 °C), suggesting that a less denatured protein population is obtained. Electrophoresis of the PKCP revealed that the protein has 11 bands with MW ranging from 2.11 to 83.19 kDa. Relative to soy protein isolate, PKCP showed higher surface hydrophobicity (165.96 vs. 51.51), better solubility at pH 7 (87.65% vs. 41.21%), oil-binding capacity (7.73 vs. 2.96 g/g) and emulsifying activity (178.50 vs. 32.57 m(2)/g), but lower water-binding capacity (0.36 vs. 11.70 g/g), emulsifying stability (32.24% vs. 43.08%), foaming capacity (20.8% vs. 100.0%) and foam stability (3.80 vs. 19.20 ml). PKCP contained the highest amount of glutamic acid (16.86 g/100 g protein) and followed by arginine (10.78 g/100 g protein). With respect to the 1991 standard of the FAO/WHO for preschool children, PKCP's essential amino acid profile showed deficiencies. Therefore, it can be used as a complementary protein source by supplementing with a tryptophan-rich source, as this was the limiting amino acid.

show abstract

“…However, temperature >60 °C is not recommended. Rustom et al. (1991) also reported that the increase in temperature from 20 to 60 °C would decrease the extracted protein.…”

Section: Resultsmentioning

confidence: 95%

Optimization of hexametaphosphate-assisted extraction and functional characterization of palm kernel cake protein

Chee

Ayob

2013

Food sci. technol. int.

View full text Add to dashboard Cite

show abstract

“…Blood, which accounts for approximately 7% of the total weight of a pig, contains 75-80% moisture and 15-17% high-quality protein (albumin, globulin, and hemoglobin) [6,7]. Despite its potential as a bioresource, blood is disposed of due to a lack of research on possible industrial uses and a general aversion.…”

Section: Pwbph Increases Endurance Performance and Relative Muscle We...mentioning

confidence: 99%

“…Blood, which accounts for approximately 7% of the total weight of a pig, contains 75-80% moisture and 15-17% high-quality protein (albumin, globulin, and hemoglobin) [6,7].…”

Section: Introductionmentioning

confidence: 99%

Effect of Porcine Whole Blood Protein Hydrolysate on Slow-Twitch Muscle Fiber Expression and Mitochondrial Biogenesis via the AMPK/SIRT1 Pathway

Jin

Lee

Kim

et al. 2022

IJMS

View full text Add to dashboard Cite

Skeletal muscle is a heterogeneous tissue composed of a variety of functionally different fiber types. Slow-twitch type I muscle fibers are rich with mitochondria, and mitochondrial biogenesis promotes a shift towards more slow fibers. Leucine, a branched-chain amino acid (BCAA), regulates slow-twitch muscle fiber expression and mitochondrial function. The BCAA content is increased in porcine whole-blood protein hydrolysates (PWBPH) but the effect of PWBPH on muscle fiber type conversion is unknown. Supplementation with PWBPH (250 and 500 mg/kg for 5 weeks) increased time to exhaustion in the forced swimming test and the mass of the quadriceps femoris muscle but decreased the levels of blood markers of exercise-induced fatigue. PWBPH also promoted fast-twitch to slow-twitch muscle fiber conversion, elevated the levels of mitochondrial biogenesis markers (SIRT1, p-AMPK, PGC-1α, NRF1 and TFAM) and increased succinate dehydrogenase and malate dehydrogenase activities in ICR mice. Similarly, PWBPH induced markers of slow-twitch muscle fibers and mitochondrial biogenesis in C2C12 myotubes. Moreover, AMPK and SIRT1 inhibition blocked the PWBPH-induced muscle fiber type conversion in C2C12 myotubes. These results indicate that PWBPH enhances exercise performance by promoting slow-twitch muscle fiber expression and mitochondrial function via the AMPK/SIRT1 signaling pathway.

show abstract

“…Therefore, the 2.5-3.0 L of blood per pig, amounting to approximately 39,216 tons per year, has a major impact on water pollution [1,2]. Blood, which accounts for approximately 7% of the total weight of pigs, generally contains 75-80% moisture and 15-17% high-quality protein; the protein is composed of albumin, globulin, and hemoglobin [3,4]. Although this blood has the potential to become a valuable bioresource, it is disposed of due to a lack of research on possible industrial uses and a general aversion to blood.…”

Section: Introductionmentioning

confidence: 99%

Effects of Porcine Whole-Blood Protein Hydrolysate on Exercise Function and Skeletal Muscle Differentiation

et al. 2021

View full text Add to dashboard Cite

A number of studies have utilized blood waste as a bioresource by enzymatic hydrolysis to obtain amino acids, such as branched-chain amino acids, which can increase muscle mass or prevent muscle loss during weight loss. Although a significantly high content of branched-chain amino acids has been reported in porcine whole-blood protein hydrolysate (PWBPH), the effects of PWBPH on skeletal muscle differentiation and exercise function remain unclear. In this study, we investigated the effects of PWBPH on exercise endurance in ICR mice and muscle differentiation in C2C12 mouse myoblasts and gastrocnemius (Gas) muscle of mice. Supplementation with PWBPH (250 and 500 mg/kg for 5 weeks) increased the time to exhaustion on a treadmill. PWBPH also increased the Gas muscle weight to body weight ratio. In addition, PWBPH treatment increased skeletal muscle differentiation proteins and promoted the Akt/mTOR-dependent signaling pathway in vitro and in vivo. These results suggest that PWBPH can be utilized as a bioresource to enhance exercise function and skeletal muscle differentiation.

show abstract

Foaming and Emulsifying Properties of Porcine Red Cell Protein Concentrate

Cited by 8 publications

References 58 publications

Optimization of hexametaphosphate-assisted extraction and functional characterization of palm kernel cake protein

Optimization of hexametaphosphate-assisted extraction and functional characterization of palm kernel cake protein

Effect of Porcine Whole Blood Protein Hydrolysate on Slow-Twitch Muscle Fiber Expression and Mitochondrial Biogenesis via the AMPK/SIRT1 Pathway

Effects of Porcine Whole-Blood Protein Hydrolysate on Exercise Function and Skeletal Muscle Differentiation

Contact Info

Product

Resources

About