Foam flotation can remove and eradicate ciliates contaminating algae culture systems

Umar, Abbas; Caldwell, Gary S.; Lee, Jonathan G.M.

doi:10.1016/j.algal.2017.12.002

Cited by 17 publications

(4 citation statements)

References 49 publications

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“…ciliates from C . vulgaris [ 83 , 84 ]. Using this technique on diatoms has the advantage of the silica frustule protecting the diatom cell membrane from the SDS action, and to bind instead to the ciliates and nematodes membranes.…”

Section: Discussionmentioning

confidence: 99%

“…Under these conditions, SDS surfactant property has been exploited for harvesting microalgae by flotation and also for contaminant removal, e.g. ciliates from C. vulgaris [83,84]. Using this technique on diatoms has the advantage of the silica frustule protecting the diatom cell membrane from the SDS action, and to bind instead to the ciliates and nematodes membranes.…”

Section: Microphytobenthic Assemblages In Hydrogelsmentioning

confidence: 99%

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Development of a new kappa-carrageenan hydrogel system to study benthic diatom vertical movements

Rizzo,

Ajò,

Kang

et al. 2024

PLoS ONE

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Benthic diatom vertical movement has been investigated mainly through indirect measurements based on chlorophyll a fluorescence and spectral reflectance signals. The presence of sediment hinders direct imaging and grazers activity renders the work under controlled conditions very difficult. This study provides a tool to study diatoms movement in a 3D hydrogel matrix. Synthetic and natural hydrogels were tested to find the best 3D transparent scaffold where diatoms could grow and freely move in all directions. Polyamidoamines (PAAm) hydrogels were no-cytocompatible and hyaluronic acid (HA) only allowed diatoms to survive for 2-days. Natural hydrogels made of gelatin/Na-alginate, Na-alginate and kappa-carrageenan (KC) were cytocompatible, with KC showing the best properties for diatom growth and movement on a long term (up to 2 months). Comparing Nitzschia spathulata, Gyrosigma limosum and Navicula phyllepta growth in liquid media vs in KC gels, we found that diatoms reached a significantly higher final biomass in the hydrogel condition. Hydrogels were also useful to isolate large size diatom species e.g., Nitzschia elongata, that did not survive in suspension. Finally, we showed three ways to study diatom species-specific movement in KC hydrogels: 1) controlled species mix; 2) natural diatom assemblages with grazers; and 3) natural diatom assemblages without grazers. With our system, single diatoms could be imaged, identified, and counted. In addition, different stimuli, e.g., light intensity and light composition can be applied and their effects on movement and physiology studied without being masked by sediment or impaired by meiofauna.

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Section: Discussionmentioning

confidence: 99%

Section: Microphytobenthic Assemblages In Hydrogelsmentioning

confidence: 99%

Development of a new kappa-carrageenan hydrogel system to study benthic diatom vertical movements

Rizzo,

Ajò,

Kang

et al. 2024

PLoS ONE

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show abstract

“…Technology however, can be used for freshwater algal cultures [124]. Umar et al (2018) evaluated foam flotation, a physiochemical method to remove ciliates Tetrahymena pyriformis from C. vulgaris culture grown in PBR. Addition of SDS at 40 mg/L concentration lysed ciliates without affecting algal cells [125].…”

Section: Pond Crashes and Mitigationmentioning

confidence: 99%

Recent Advances in Algal Biomass Production

Rajvanshi¹,

Sayre²

2021

Biotechnological Applications of Biomass

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The promise of algae to address the renewable energy and green-product production demands of the globe has yet to be realized. Over the past ten years, however, there has been a substantial investment and interest in realizing the potential of algae to meet these needs. Tremendous progress has been achieved. Ten years ago, the price of gasoline produced from algal biomass was 20-fold greater than it is today. Technoeconomic models indicate that algal biocrude produced in an optimized cultivation, harvesting, and biomass conversion facility can achieve economic parity with petroleum while reducing carbon-energy indices substantially relative to petroleum-based fuels. There is also an emerging recognition that algal carbon capture and sequestration as lipids may offer a viable alternative to direct atmospheric CO2 capture and sequestration. We review recent advances in basic and applied algal biomass production from the perspectives of algal biology, cultivation, harvesting, energy conversion, and sustainability. The prognosis is encouraging but will require substantial integration and field testing of a variety of technology platforms to down select the most economical and sustainable systems to address the needs of the circular economy and atmospheric carbon mitigation.

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“…Though SDS electrophoresis has been utilised for preparative separation of collagen [125], it has been mainly used to compare collagen from different tissue types and to identify collagen types and polypeptide chains. Wu et al [126] extracted bovine collagen and applied SDS-PAGE to identify the different collagen types present.…”

Section: Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (...mentioning

confidence: 99%

Biotechnological Applications of Biomass

Basso¹,

Basso²,

Basso³

2021

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Over the last century, there has been increasing debate concerning the use of biomass for different purposes such as foods, feeds, energy fuels, heating, cooling and most importantly biorefinery feedstock. The biorefinery products were aimed to replace fossil fuels and chemicals as they are renewable form of energy. Biomass is a biodegradable product from agricultural wastes and residues, forestry and aquaculture. Biomass could be sourced from a variety of raw materials such as wood and wood processing by-products, manure, fractions of organic waste products and agricultural crops. As a form of renewable energy, they have the advantages of easy storage, transportation, flexible load utilization and versatile applications. The aim of this study is to provide an overview for thermochemical and biochemical biomass conversion technologies that were employed currently. Attention was also paid to manufacture of biofuels because of their potentials as key market for largescale green sustainable biomass product.

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Foam flotation can remove and eradicate ciliates contaminating algae culture systems

Abstract: This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International licence Newcastle University ePrints -eprint.ncl.ac.uk Umar A, Caldwell GS, Lee JM. Foam flotation can remove and eradicate ciliates contaminating algae culture systems.

Cited by 17 publications

References 49 publications

Development of a new kappa-carrageenan hydrogel system to study benthic diatom vertical movements

Development of a new kappa-carrageenan hydrogel system to study benthic diatom vertical movements

Recent Advances in Algal Biomass Production

Biotechnological Applications of Biomass

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