FlyBase: establishing a Gene Group resource for<i>Drosophila melanogaster</i>

Attrill, Helen; Falls, Kathleen; Goodman, Joshua L.; Millburn, Gillian; Antonazzo, Giulia; Rey, Alix J.; Marygold, Steven J

doi:10.1093/nar/gkv1046

Cited by 299 publications

(366 citation statements)

References 19 publications

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“…Fusion constructs containing regions 2 or 3 robustly activated transcription from T1, but not T2 or T3, suggesting that these basal elements are unlikely to generate much of the overall transcriptional output, a conclusion supported by RNA-seq analysis ( Fig. S5) (Graveley et al, 2011;Brown et al, 2014;Attrill et al, 2016). Previous studies have focused largely on the regulatory potential of T2 (Puig et al, 2003;Casas-Tinto et al, 2007); our analysis indicates that much of the regulatory activity of this locus is likely to be channeled through the distal T1 promoter.…”

Section: Identification Of Active Enhancers Within Inr Intronssupporting

confidence: 67%

“…S4). This basal promoter appears to be the predominant site of initiation (Casas-Tinto et al, 2007;Graveley et al, 2011;Brown et al, 2014;Attrill et al, 2016). We compared the promoter activities of T1, T2 and T3 in luciferase reporter constructs, using a similarly sized intronic fragment (PT) as a negative control ( Fig.…”

Section: Identification Of Active Enhancers Within Inr Intronsmentioning

confidence: 99%

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Complex cis-regulatory landscape of the insulin receptor gene underlies the broad expression of a central signaling regulator

et al. 2016

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Insulin signaling plays key roles in development, growth and metabolism through dynamic control of glucose uptake, global protein translation and transcriptional regulation. Altered levels of insulin signaling are known to play key roles in development and disease, yet the molecular basis of such differential signaling remains obscure. Expression of the insulin receptor (InR) gene itself appears to play an important role, but the nature of the molecular wiring controlling InR transcription has not been elucidated. We characterized the regulatory elements driving Drosophila InR expression and found that the generally broad expression of this gene is belied by complex individual switch elements, the dynamic regulation of which reflects direct and indirect contributions of FOXO, EcR, Rbf and additional transcription factors through redundant elements dispersed throughout ∼40 kb of non-coding regions. The control of InR transcription in response to nutritional and tissuespecific inputs represents an integration of multiple cis-regulatory elements, the structure and function of which may have been sculpted by evolutionary selection to provide a highly tailored set of signaling responses on developmental and tissue-specific levels.

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Section: Identification Of Active Enhancers Within Inr Intronssupporting

confidence: 67%

Section: Identification Of Active Enhancers Within Inr Intronsmentioning

confidence: 99%

Complex cis-regulatory landscape of the insulin receptor gene underlies the broad expression of a central signaling regulator

et al. 2016

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“…The Drosophila CG45057 locus, named ringmaker (ringer), at cytological position 72E2 (Attrill et al, 2015) was uncovered through a deficiency screen aimed at identifying genes involved in VNC development (R.M. and M.B., unpublished).…”

Section: Drosophila Cg45057 Locus Encodes Ringer a Homolog Of Mammalmentioning

confidence: 99%

Drosophila Ringmaker regulates microtubule stabilization and axonal extension during embryonic development

Mino

Rogers

Risinger

et al. 2016

Journal of Cell Science

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Axonal growth and targeting are fundamental to the organization of the nervous system, and require active engagement of the cytoskeleton. Polymerization and stabilization of axonal microtubules is central to axonal growth and maturation of neuronal connectivity. Studies have suggested that members of the tubulin polymerization promoting protein (TPPP, also known as P25α) family are involved in cellular process extension. However, no in vivo knockout data exists regarding its role in axonal growth during development. Here, we report the characterization of Ringmaker (Ringer; CG45057), the only Drosophila homolog of long p25α proteins. Immunohistochemical analyses indicate that Ringer expression is dynamically regulated in the embryonic central nervous system (CNS). ringer-null mutants show cell misplacement, and errors in axonal extension and targeting. Ultrastructural examination of ringer mutants revealed defective microtubule morphology and organization. Primary neuronal cultures of ringer mutants exhibit defective axonal extension, and Ringer expression in cells induced microtubule stabilization and bundling into rings. In vitro assays showed that Ringer directly affects tubulin, and promotes microtubule bundling and polymerization. Together, our studies uncover an essential function of Ringer in axonal extension and targeting through proper microtubule organization.

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“…The aminopeptidase N gene of Drosophila is located on 3R at polytene bands 97D7-97D9 (Attrill et al 2016). Although Zhang et al (2002) performed a thorough genetic analysis of the mutant with existing x-ray-induced deficiencies, we observed a higher than expected recombination frequency (14.7%) between sda iso7.8 and the transposon insertion line P[GawB]386Y, which has an insertion site near the gene amon at polytene bands 97C4-97C5 and sda iso7.8 complemented molecularly defined deficiencies from the aminopeptidase N locus.…”

mentioning

confidence: 99%

julius seizure, a Drosophila Mutant, Defines a Neuronal Population Underlying Epileptogenesis

et al. 2017

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Epilepsy is a neural disorder characterized by recurrent seizures. Bang-sensitive Drosophila represent an important model for studying epilepsy and neuronal excitability. Previous work identified the bang-sensitive gene slamdance (sda) as an allele of the aminopeptidase N gene. Here we show through extensive genetic analysis, including recombination frequency, deficiency mapping, transposon insertion complementation testing, RNA interference (RNAi), and genetic rescue that the gene responsible for the seizure sensitivity is julius seizure (jus), formerly CG14509, which encodes a novel transmembrane domain protein. We also describe more severe genetic alleles of jus. RNAi-mediated knockdown of jus revealed that it is required only in neurons and not glia, and that partial bang-sensitivity is caused by knockdown in GABAergic or cholinergic but not glutamatergic neurons. RNAi knockdown of jus at the early pupal stages leads to strong seizures in adult animals, implicating that stage as critical for epileptogenesis. A C-terminal-tagged version of Jus was generated from a fosmid genomic clone. This fosmid fusion rescued the bang-sensitive phenotype and was expressed in the optic lobes and the subesophageal and thoracic abdominal ganglia. The protein was primarily localized in axons, especially in the neck connectives, extending into the thoracic abdominal ganglion.

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FlyBase: establishing a Gene Group resource forDrosophila melanogaster

Cited by 299 publications

References 19 publications

Complex cis-regulatory landscape of the insulin receptor gene underlies the broad expression of a central signaling regulator

Complex cis-regulatory landscape of the insulin receptor gene underlies the broad expression of a central signaling regulator

Drosophila Ringmaker regulates microtubule stabilization and axonal extension during embryonic development

julius seizure, a Drosophila Mutant, Defines a Neuronal Population Underlying Epileptogenesis

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