[1] As methane is consumed in the deep sea, its 13 C/ 12 C ratio progressively increases because of kinetic isotope fractionation. Many submarine hydrothermal vents emit methane with carbon isotope ratios that are higher than those of background methane in the surrounding ocean. Since the latter exists at low concentrations, mixing of background methane with vent fluid tends to decrease the 13 C/ 12 C ratio as concentration decreases, opposite to the trend produced by consumption. We investigated CH 4 concentration and d 13 C together with d 3 He in plumes from the Logatchev hydrothermal field (LHF) located at 14°45 0 N, 45°W, which generates relatively heavy methane (d 13 C % À13%) by serpentinization of ultramafic rock. The measured methane and d 3 He were well correlated at high concentrations, indicating a CH 4 / 3 He ratio of 1 Â 10 8 in the vent fluids. These tracer distributions were also simulated with an advection-diffusion model in which methane consumption only occurs above a certain threshold concentration. We utilized d 3 He to calculate the methane remaining in solution after oxidation, f, and the deviation of d 13 C from the value expected from mixing alone, Dd 13 C. Both in the model and in the data, the entire set of Dd 13 C values are not correlated with log f, which is due to continuous oxidation within the plume while mixing with background seawater. A linear relationship, however, is found in the model for methane at concentrations sufficiently above background, and many of the samples with elevated CH 4 north of LHF exhibit a linear trend of Dd 13 C versus log f as well. From this trend, the kinetic isotope fractionation factor in the LHF plumes appears to be about 1.015. This value is somewhat higher than found in some other deep-sea studies, but it is lower than found in laboratory incubation experiments.