Fluroxypyr- and Triclopyr-Specific Enzyme-Linked Immunosorbent Assays:  Development and Quantitation in Soil and Water

and, B. Dee Johnson; Hall, J. Christopher

doi:10.1021/jf9405867

Cited by 23 publications

(17 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The enzyme-linked immunosorbent assay (ELISA) is recognized as a valuable tool in residue analysis and compliments conventional analytical methods (Johnson and Hall, 1996;Parnell and Hall, 1998). ELISA provides rapid sample testing and accurate results and is more cost-effective than conventional chromatographic analysis (Hall et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Development of an Enzyme-Linked Immunosorbent Assay for the Detection of Glyphosate

Clegg

Stephenson

Hall

1999

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

A competitive indirect enzyme-linked immunosorbent assay (CI-ELISA) was developed to quantitate the herbicide glyphosate [N-(phosphonomethyl)glycine] in water. The ELISA has a detection limit of 7.6 microg mL(-1) and a linear working range of 10-1000 microg mL(-1) with an IC(50) value of 154 microg mL(-1). The glyphosate polyclonal antisera did not cross-react with a number of other herbicides tested but did cross-react with the glyphosate metabolite aminomethylphosphonic acid and a structurally related herbicide, glyphosine [(N,N-bis(phosphonomethyl)glycine]. The assay was used to estimate, quantitatively with accuracy and precision, glyphosate concentrations in water samples. Water samples were analyzed directly, and no sample preparation was required. To improve detection limits, water samples were concentrated prior to analysis, resulting in the increase of the detection limits by 100-fold. After the sample preconcentration step, the detection limit improved to 0.076 microg mL(-1) with an IC(50) value of 1.54 microg mL(-1), and a linear working range was 0.1-10 microg mL(-1). Glyphosate concentrations determined by ELISA correlated well with those determined by high-pressure liquid chromatography (r(2) = 0.99). This assay contributes to reducing the costs associated with conventional residue analysis techniques for the quantitation of glyphosate in water.

show abstract

Section: Introductionmentioning

confidence: 99%

Development of an Enzyme-Linked Immunosorbent Assay for the Detection of Glyphosate

Clegg

Stephenson

Hall

1999

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The dissipation dynamics of fluroxypyrmeptyl in soil in Beijing and Tianjin could be described by Residue Analysis of Fluroxypyr-meptyl 293 According to the literature, fluroxypyr-meptyl could hydrolyze to the acid form (fluroxypyr) [1,2]. Tao [4] and Bai [5] developed analytical methods for fluroxypyr residues in wheat, in which the recoveries ranged from 84.0 to 94.1%.…”

Section: Dissipation Of Fluroxypyr-meptyl In Soilmentioning

confidence: 99%

“…Fluroxypyr is mostly applied as the methylheptyl ester [fluroxypyr-meptyl ( Fig. 1)] in China [2]. To the best of our knowledge, the analytical method of residues of fluroxypyr-meptyl was not been found in any literature, although numerous of analytical methods for fluroxypyr residues have been published [3][4][5][6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Residue Analysis of Fluroxypyr-meptyl in Wheat and Soil by GC–ECD

Zhen

et al. 2011

Chromatographia

View full text Add to dashboard Cite

A simple and reliable analytical method for fluroxypyr-meptyl residues in wheat and soil was established. The residual levels and dissipation rates of this pesticide in wheat and soil were determined by gas chromatography with electron capture detector (GC-ECD). Samples of wheat grains and soil were extracted with methanol-water, and then liquid-liquid partitioned with dichloromethane. The extracts were further cleaned up by solid-phase extraction (SPE) with Florisil cartridges (500 mg, 3 mL), and eluted with petroleum ether-acetic ether (95/5, v/v). The eluate was collected and concentrated for GC-ECD analysis. The results showed good linearity (r 2 = 0.999) over the concentration range of 0.01-1.0 lg mL -1 . Limits of quantification (LOQ) of the method were 0.005 mg kg -1 for both soil and wheat grains. Average recoveries for the wheat grains and soil samples were 84.45-108.2% and 95.33-106.6%, respectively, and the corresponding relative standard deviations (RSDs) of the recovery data were 0.819-3.77% and 1.55-5.28% for the three fortified levels (0.005, 0.1, 0.5 mg kg -1 ). The half-life of fluroxypyr-meptyl in soil from experimental field was 1.93 days (in Beijing) and 3.01 days (in Tianjin). The final residue of the pesticide in wheat and soil was lower than 0.005 mg kg -1 , respectively, at harvest time. The analytes in real samples were further confirmed by gas chromatography-mass spectrometry (GC-MS). It was demonstrated that the proposed method was simple and efficient, and particularly suitable for detecting fluroxypyr-meptyl residues in wheat and soil.

show abstract

“…Modification of the structure of the coating conjugate hapten is routinely used to increase the sensitivity of immunoassays for residue analysis (Harrison et al, 1989;Marco et al, 1995;Schneider and Hammock, 1992;Wie and Hammock, 1984). For example, Johnson and Hall (1996) report an enhancement in sensitivity and reduction in cross-reactivity of a triclopyr ELISA from 5 to 0.1 ng/L by modifying the direct ELISA format from a homologous to a heterologous assay. In cross-reactivity studies, Webb and Hall (2000) also showed that higher concentrations of nonactive cyclohexanediones (IC 50 > 100 µM; Markley et al, 1995; Webb et al, 1997) were required to compete with CCA for antibody binding, as compared with active cyclohexanediones (IC 50 < 100 µM).…”

Section: Rationale For Modification Of Cyclohexadionementioning

confidence: 99%

Development and Evaluation of an Immunological Approach for the Identification of Novel Acetyl Coenzyme-A Carboxylase Inhibitors: Assay Optimization and Pilot Screen Results

Webb

Hall

2000

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

Cyclohexanediones, aryloxyphenoxypropionates, indolizidinediones, and triazinediones are four known structural classes of herbicides that inhibit acetyl coenzyme-A carboxylase (ACCase; EC 6.4.1.2). An immunological study to determine the potential of ACCase inhibitor-specific monoclonal antibodies as screening tools to identify novel lead chemistry was undertaken. Using two cyclohexanedione-specific monoclonal antibodies (mAb A and mAb B; Webb, S. R.; Hall, J. C. J. Agric. Food Chem. 2000, 48, 1210-1218) and three different cyclohexanedione hapten coating conjugates, competitive indirect enzyme-linked immunosorbent assays (ciELISA) were developed. Cross-reactivity of the monoclonal antibodies with four structural classes of ACCase inhibitors revealed that the ciELISA using mAb A and a modified cyclohexanedione hapten coating conjugate detected analogues from all four known classes of ACCase inhibitors. A pilot screen using this ciELISA format identified two novel ACCase inhibitors, demonstrating the potential for antibodies as rapid and cost-effective screening tools for identifying novel lead chemistry in pesticide discovery programs.

show abstract

Fluroxypyr- and Triclopyr-Specific Enzyme-Linked Immunosorbent Assays: Development and Quantitation in Soil and Water

Cited by 23 publications

References 43 publications

Development of an Enzyme-Linked Immunosorbent Assay for the Detection of Glyphosate

Development of an Enzyme-Linked Immunosorbent Assay for the Detection of Glyphosate

Residue Analysis of Fluroxypyr-meptyl in Wheat and Soil by GC–ECD

Development and Evaluation of an Immunological Approach for the Identification of Novel Acetyl Coenzyme-A Carboxylase Inhibitors: Assay Optimization and Pilot Screen Results

Contact Info

Product

Resources

About