Fluorouracil (5-FU) and Cytarabine (Ara-C) Inhibition of Corneal Epithelial Cell and Conjunctival Fibroblast Proliferation

Mallick, Kim S.; Hajek, Anthony S.; Parrish, Richard K.

doi:10.1001/archopht.1985.01050090150052

Cited by 53 publications

(21 citation statements)

References 23 publications

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“…Alkylating agents, including antipyrimidines, antineoplastic antibiotics and corticosteroids have been used to inhibit this scar formation in vivo and in vitro [11, 12, 13]. Among them, 5-fluorouracil (5-FU) and mitomycin C (MMC) are the most favorable [14, 15, 16].…”

Section: Introductionsupporting

confidence: 45%

The Effect of Hydroxyurea on Rabbit Subconjunctival Fibroblast Culture and Use of Hydroxyurea in Rabbits after Glaucoma Filtration Surgery

Yücel¹,

Bağcı

Duranoğlu³

et al. 1999

Ophthalmologica

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In an in vitro study, rabbit subconjunctival fibroblasts were cultured and the effects of an antineoplastic drug, hydroxyurea (HU), on fibroblast proliferation and fibroblast attachment was investigated. The effects of HU were compared with those of mitomycin C (MMC). Different concentrations of HU and MMC were added to culture medium. The HU doses which led to 50% of inhibition (ID₅₀) and the dose which led to about 90% of inhibition (subtoxic high dose, STHD) were determined to be 8 and 1,000 μg/ml, respectively. ID₅₀ of MMC and its STHD which led to about 100% inhibition were found to be 0.01 and 1 μg/ml, respectively. Reversibility studies revealed that inhibition disappeared depending on the dose and incubation period of both HU and MMC. In an in vivo study, glaucoma filtration surgery (GFS) was performed in rabbits which were treated with HU (treatment group) and distilled water (control group). Tissue samples were taken from the subconjunctival area treated at 1 h, 1 day, 5 days and 30 days postoperatively. The biopsy specimens were then placed in tissue culture media. Fibroblast outgrowth rates detected in the HU group were found to be significantly lower than those in the control group in the specimens taken at the end of the first hour. The difference was significant on culture days 9–15 in the biopsy specimens taken on day 1 while it was not significant in those taken on days 5 and 30.

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Section: Introductionsupporting

confidence: 45%

The Effect of Hydroxyurea on Rabbit Subconjunctival Fibroblast Culture and Use of Hydroxyurea in Rabbits after Glaucoma Filtration Surgery

Yücel¹,

Bağcı

Duranoğlu³

et al. 1999

Ophthalmologica

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“…According to Rootman et al6 and Fantes et al,5 who studied intraocular penetration of this drug using fluorouracil labeled with carbon 14, the fluorouracil concentration in the anterior chamber ranged from 69.5 to 65.7 mg/L at one hour after the subconjunctival injection of 12.5 mg of fluorouracil. This figure is not far from the present result, 19.1 mg/L one hour after subconjunctival injection of 5 mg of fluorouracil. However, at two hours or later, the fluorouracil levels in the anterior chamber, pres¬ ently obtained, were considerably lower than those estimated from the results of Rootman et al6; the above discrepancy between the present results and theirs may indicate that two hours or more after the subcon¬ junctival injection, a significant por¬ tion of the radioactivity measured in the ocular tissue represents the inac¬ tive metabolites and degradation products of fluorouracil.…”

Section: Resultsmentioning

confidence: 46%

Concentration Change of Fluorouracil in the External Segment of the Eye After Subconjunctival Injection

Kondo¹

1988

Arch Ophthalmol

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“…15,16 5FU induces apoptosis of the Tenon's fibroblasts and has anti-proliferative fibroblastic properties. 17,18 In ophthalmology, 5FU is widely used because of its anti-scarring properties. In 2007, we reported a single case of multiple post-surgical recurrences of pterygium in an Afro-Caribbean lady who was successfully treated with intra-lesional (5FU) injection following the fourth operation.…”

Section: Introductionmentioning

confidence: 43%

Intra-lesional 5 fluorouracil for the management of recurrent pterygium

Said

Faraj

El‐Alfy

et al. 2013

Eye

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Aim Recurrence is the most common complication arising from pterygium surgery. The aim of this study was to investigate the effectiveness of 5 fluorouracil (5FU) in halting the recurrence of pterygium after surgical excision. Methods A retrospective review of patients treated for pterygium recurrence was carried out. Patients with recurrent (secondary) pterygium were treated with multiple weekly intra-lesional injections of 0.1-0.2 ml (2.5-5 mg) 5FU post-operatively depending on the size of the recurrence. The treatment was started within 1 month from the date of recurrence. The time from surgery to start of recurrence, previous treatment modalities, and number of recurrences were documented. The number of injections required to induce arrest of progression and/ or regression of vascularity and fleshiness of the pterygium and any complications related to 5FU treatment were examined. Results Fifteen eyes from 14 patients with recurrent pterygium treated with intralesional 5FU injections were analysed. Three of the 15 eyes had undergone a secondary excision and 12 had undergone a primary excision. In all, 93.3% of patients showed regression of the fibrovascular tissue (thickness and vascularity) and arrest of progression following a dose of 0.1-0.2 ml (2.5-5 mg) 5FU. Twelve eyes required three injections or fewer, whereas one patient required eight injections. This beneficial effect was maintained over an average follow-up period of 17 months. No complications from 5FU were observed. Conclusion The use of weekly intra-lesional 5FU injections for the treatment of recurrent pterygium is safe and effective in limiting the progression and inducing the regression of recurrent pterygium. The number of injections can be tailored according to clinical need.

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Fluorouracil (5-FU) and Cytarabine (Ara-C) Inhibition of Corneal Epithelial Cell and Conjunctival Fibroblast Proliferation

Cited by 53 publications

References 23 publications

The Effect of Hydroxyurea on Rabbit Subconjunctival Fibroblast Culture and Use of Hydroxyurea in Rabbits after Glaucoma Filtration Surgery

The Effect of Hydroxyurea on Rabbit Subconjunctival Fibroblast Culture and Use of Hydroxyurea in Rabbits after Glaucoma Filtration Surgery

Concentration Change of Fluorouracil in the External Segment of the Eye After Subconjunctival Injection

Intra-lesional 5 fluorouracil for the management of recurrent pterygium

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