Fluorosurfactants for microdroplets: Interfacial tension analysis

Holt, Daniel J.; Payne, Richard J.; Chow, Wing Ying; Abell, Chris

doi:10.1016/j.jcis.2010.06.036

Cited by 37 publications

(46 citation statements)

References 27 publications

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“…Currently, one commercial supplier (RainDance Technologies) exists for PFPE-PEG block copolymer surfactants, 25 but studies demonstrate small-scale synthesis techniques of a number of surfactant groups such as PFPE-HEG. 29,30 Alternatives such as light mineral oil have been utilized in biological drop generation applications to access a wider range of available surfactants, 24,31 but note that the accompanying increase in viscosity as compared to fluorocarbon oil alters the drop generation parameters. A recent review 32 describes a large number of published continuous phase oils and surfactants.…”

Section: Discussionmentioning

confidence: 99%

High Throughput Single-cell and Multiple-cell Micro-encapsulation

Lagus

2012

JoVE

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Microfluidic encapsulation methods have been previously utilized to capture cells in picoliter-scale aqueous, monodisperse drops, providing confinement from a bulk fluid environment with applications in high throughput screening, cytometry, and mass spectrometry. We describe a method to not only encapsulate single cells, but to repeatedly capture a set number of cells (here we demonstrate one-and two-cell encapsulation) to study both isolation and the interactions between cells in groups of controlled sizes. By combining drop generation techniques with cell and particle ordering, we demonstrate controlled encapsulation of cell-sized particles for efficient, continuous encapsulation. Using an aqueous particle suspension and immiscible fluorocarbon oil, we generate aqueous drops in oil with a flow focusing nozzle. The aqueous flow rate is sufficiently high to create ordering of particles which reach the nozzle at integer multiple frequencies of the drop generation frequency, encapsulating a controlled number of cells in each drop. For representative results, 9.9 μm polystyrene particles are used as cell surrogates. This study shows a single-particle encapsulation efficiency Pk=1 of 83.7% and a double-particle encapsulation efficiency Pk=2 of 79.5% as compared to their respective Poisson efficiencies of 39.3% and 33.3%, respectively. The effect of consistent cell and particle concentration is demonstrated to be of major importance for efficient encapsulation, and dripping to jetting transitions are also addressed.

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Section: Discussionmentioning

confidence: 99%

High Throughput Single-cell and Multiple-cell Micro-encapsulation

Lagus

2012

JoVE

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“…Moreover, an elevated temperature intensifies the Brownian motion and it is known that, the lower the cohesive energy, the smaller the interfacial tension. Furthermore, nanoparticles can be adsorbed at the liquid–liquid interface and function in similar way to the surfactants to reduce the surface tension or interfacial tension (Holt et al 2010). Similar to the alteration of thermophysical properties (Muradoglu and Tryggvason 2008; Murshed et al 2008b), the addition of a small amount of nanoparticles into the liquid can change obviously its surface properties such as interfacial tensions.…”

Section: Resultsmentioning

confidence: 99%

Nanoparticles influence droplet formation in a T-shaped microfluidic

Wang

2013

J Nanopart Res

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Droplet formation in the presence of nanoparticles was studied in a T-shaped microfluidic device numerically. Nanoparticles in continuous phase did not influence droplet formation dynamics obviously. Contrarily, the presence of nanoparticles in dispersed phase will influence evidently droplet formation dynamics, likely reasons are the accumulation of nanoparticles at the liquid–liquid interface leading to the variation of interfacial tension and the anisotropy of nanoparticles’ movement at interface. The droplet size decreases almost linearly with increasing of the volume fraction of nanoparticles in dispersed phase when the volume fraction of nanoparticles not exceeding a critical value (about 0.2 %), because very high concentration of nanoparticles results in particle aggregation so as to not decrease interfacial tension so obviously any more. A complicated mechanism of temperature influences on droplet formation may exist combining the variations of effective viscosity and interfacial tension. Discussions on microscopic mechanism of droplet formation in the presence of nanoparticles were carried out.

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“…[46,53] PFPE-Hexaethylenglycol eignete sich besonders gut zur Stabilisierung von wässrigen Trçpfchen und Trçpfchen aus Acetonitril. Insbesondere die beiden letzteren Gruppen brachten vielversprechende Ergebnisse bei der Stabilisierung von organischen Lçsungsmitteltrçpfchen.…”

Section: Trçpfchenstabilität Biokompatibilität Und Verlustunclassified

“…Abhängig davon, in welchem Ausmaß eine Mehrfachbesetzung in einer Anwendung toleriert wird, kann die Zellkonzentration jedoch auf eine bestimmte Poisson-Verteilung eingestellt werden, zum Beispiel auf eine Besetzung von 90, 9 und 1 % oder 40, 30 und 30 % bei einem Verhältnis von Zellen zu Trçpfchen von 0.1 beziehungsweise > 1. Um die Poisson-Verteilung der besetz-ten Trçpfchen zu umgehen, wurden Hochdurchsatz-Injektionsmethoden entwickelt, [43] [46] Baret [47] erläutert in seinem Aufsatz ausführlich die Rolle der Tenside in mikrofluidischen Trçpfchensystemen. Abbildung 2.…”

Section: Verkapselung Von Einzelzellenunclassified

Tröpfchen‐Mikrofluidik für die Einzelzellanalyse

Joensson

Svahn

2012

Angewandte Chemie

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Die tröpfchenbasierte Mikrofluidik dient der Isolierung und Manipulation von einzelnen Zellen und Reagentien innerhalb von monodispersen, pikolitergroßen Flüssigkapseln bei einem Umsatz von tausenden Tröpfchen pro Sekunde. Diese Qualitäten machen die Tröpfchen‐Mikrofluidik geeignet für viele Anforderungen der Einzelzellanalyse. Durch die Monodispersität lässt sich die Konzentration in den Tröpfchen quantitativ einstellen. Die Tröpfchen bieten der Zelle und ihrer unmittelbaren Umgebung ein isoliertes Kompartiment, und bei einem Durchsatz von tausenden Tröpfchen pro Sekunde ist es möglich, zehntausende bis millionen verkapselte Zellen zu prozessieren. Heterogene Zellpopulationen lassen sich somit exakt beschreiben oder seltene Zellarten identifizieren. Das kleine Volumen der Tröpfchen macht auch sehr große Screenings ökonomisch machbar. Dieser Aufsatz gibt einen Überblick über den aktuellen Stand der Einzelzellanalyse durch die Tröpfchen‐Mikrofluidik und nennt Beispiele, bei denen sie biologische Vorgänge besser verstehen hilft.

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Fluorosurfactants for microdroplets: Interfacial tension analysis

Cited by 37 publications

References 27 publications

High Throughput Single-cell and Multiple-cell Micro-encapsulation

High Throughput Single-cell and Multiple-cell Micro-encapsulation

Nanoparticles influence droplet formation in a T-shaped microfluidic

Tröpfchen‐Mikrofluidik für die Einzelzellanalyse

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