Fluorospectrometric studies on neothramycin and its reaction with DNA.

Maruyama, Ichiro; Tanaka, Nobuo; Kondo, Shinichi; Umezawa, Hamao

doi:10.7164/antibiotics.34.427

Cited by 6 publications

(1 citation statement)

References 18 publications

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“…The higher binding of sanguinarine to DNA at acidic pH value may support its effectiveness on tumour cells where the average pH is lower than normal tissue ( 45,46). The similar pH dependent binding has also been obtained in ellipticine-DNA complex ( 47) and neothramycin-DNA complex ( 48). Therefore, a detailed description of the conformational aspect of the nature of the interaction between intercalated sanguinarine and double-stranded Downloaded by [New York University] at 20:17 03 February 2015 DNA is, of course, essential for eventually arriving at a better understanding of the relationship between the structure of the sanguinarine -DNA complex, and the known biological properties of sanguinarine.…”

Section: Discussionmentioning

confidence: 55%

Circular Dichroism of Sanguinarine—DNA Complexes: Effect of Base Composition, pH and Ionic Strength

Maiti¹,

Nandi²

1987

Journal of Biomolecular Structure and Dynamics

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The interaction of sanguinarine with various naturally occurring and synthetic deoxyribonucleic acids of different base composition and sequence has been studied from the measurement of circular dichroism spectroscopy in buffer of various ionic strengths and pH values where physico-chemical properties of DNA remain unchanged. Binding of sanguinarine to DNA causes a change in the circular dichroism spectrum of DNA itself by showing the increase of both positive and negative bands and appearance of a broad positive band with peak at 340 nm. All systems studied exhibit extrinsic positive band that is independent of DNA base composition and sequence. Calf thymus DNA-sanguinarine complexes in different salt concentrations show that, at saturation, the magnitude of molar ellipticity at 340 nm increases with decreasing sodium ions concentration. Sanguinarine-DNA complexes in buffer of various pH values also show that the molar ellipticity at 340 nm is greater in acidic pH and lower in alkaline pH. It is concluded that the alterations of secondary structure of DNA upon binding of sanguinarine is maximum in buffer of low ionic strength and acidic pH and the number of bound alkaloid molecules per base pair, at saturation, is more in G-C rich DNA than in A-T rich DNA.

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