Fluoroquinolone therapy in a rabbit model of post-LASIK methicillin-resistant Staphylococcus aureus keratitis

Abstract

Methicillin-resistant S aureus inoculation beneath a rabbit corneal flap produced an infection that was useful for quantitative microbiological studies. A significant advantage in using moxifloxacin relative to gatifloxacin was observed in prophylaxis of keratitis (P = .0001).

Animal Models of Bacterial Keratitis

“…Alternatives to the intrastromal model include soaking contact lenses in S. aureus prior to placement on wounded rabbit corneas [84, 93–95] and induction of a post-LASIK model of keratitis in which S. aureus was inoculated underneath rabbit corneal flaps that mimic LASIK surgery [96] and other corneal flap models [97–99]. …”

Leukocyte-endothelial interactions within the ocular microcirculation in inflammation and infection

“…[47] • Cytokines (e.g. IL-1) in development of ocular disease, by depletion studies [47] Antibiotics [19] Corneal scratch and topical administration in • Infiltration of PMN cell to corneal stroma [29] Ciprofloxacin [115] mice [29,69,92], and rabbits [13] • Lectin in bacterial adherence (at 1 h) [13] Contact lens-induced keratitis in guinea pigs [131], rats [120,122], in rabbits [62,120] • Infiltration of immune cells [131], PMNs [120], T cells, phagocytes and macrophages [122] • Pro-inflammatory cytokines (IL-1, IL-6) [120] Topical administration after corneal flap surgery in rabbits [53,139] Chelation [142] Staphylococcus Intravitreal/intrastromal injection in rats [40] • Infiltration of PMNs, the depletion of which reduces the clinical disease [40] Staphylolysin [7] Corneal scratch and topical application in mice (strains and ages of mice vary) [41,57] • Infiltration of PMNs (for up to 9 d) [41,57] • Cytokine responses [57] Corneal Scratch and contact lens in rabbit (using bacteria, virulence factors) [29,56,141] • Infiltration of leukocytes (after 24 h) [141] Melamine coated lens [30] Surgical procedures in rabbits [6,94,130] Antibiotic [6,94,...…”

Normal corneal thickness measurements in pigmented rabbits using spectral‐domain anterior segment optical coherence tomography

“…The spectral-domain AS-OCT results in improved resolution of the B-scan images, better delineation of the cornea layers, and more precise data about the measurement, but has a shorter scan length and depth than timedomain AS-OCT. 5 Laser-assisted in situ keratomileusis (LASIK) is one of the most frequent ophthalmic refractive surgeries performed on human corneas, and rabbit models are often used in related LASIK research. [6][7][8][9] Therefore, it is important to know the normal corneal thickness pre-or post-refractive operations in these animals; the spectraldomain AS-OCT plays an important, reliable, and convenient role in assessing this data. 10 Given the importance of the rabbit as an animal research species in ophthalmology 11,12 and in veterinary medicine, 13,14 gathering data from AS-OCT readings in these animals can assist in future research.…”