Fluorophores’ talk turns them dark

Almahayni, Karim; Spiekermann, Malte; Möckl, Leonhard

doi:10.1038/s41592-022-01565-5

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2023

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“…Therefore, SMLM has the potential for adoption as a routine microscopy system in biomedical research. The recipe for high-quality SMLM reconstructions is: (i) a sample where the structure of interest is labeled densely with bright, switchable fluorophores as poor sampling causes poor reconstructions, , (ii) efficient detection, and (iii) precise and accurate localization. Of course, for accurate localization, proper software must be used .…”

Section: Introductionmentioning

confidence: 99%

Simple, Economic, and Robust Rail-Based Setup for Super-Resolution Localization Microscopy

Almahayni,

Nestola,

Spiekermann

et al. 2023

J. Phys. Chem. A

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Research during the past 2 decades has showcased the power of single-molecule localization microscopy (SMLM) as a tool for exploring the nanoworld. However, SMLM systems are typically available in specialized laboratories and imaging facilities, owing to their expensiveness as well as complex assembly and alignment procedure. Here, we lay out the blueprint of a sturdy, rail-based, cost-efficient, multicolor SMLM setup that is easy to construct and align in service of simplifying the accessibility of SMLM. We characterize the optical properties of the design and assess its capabilities, robustness, and stability. The performance of the system is assayed using super-resolution imaging of biological samples. We believe that this design will make SMLM more affordable and broaden its availability.

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Section: Introductionmentioning

confidence: 99%