Fluorometrical analysis of guanidino compounds in mouse brain

Fluorometrical Analysis of Guanidino Compounds in Human Cerebrospinal Fluid

“…It is well known that guanidinosuccinic acid accumulates in renal deficiency (Cohen et al, 1968). We (Mori et al, 1979) observed trace amounts of guanidinosuccinic acid in normal mice brain. In the present study, we detected trace amounts (up to 0.2 nmol/ml) of guanidinosuccinic acid in 13 of the 57 human subjects.…”

“…The fluorescence-inducing reagent was 0.05% 9,IO-phenanthrenequinone in dimethylformamide. Other details have been described elsewhere (Mori et al, 1979).…”

Akitane Mori, the Artistic Neurochemist

“…In 1970, Akitane was appointed as an associate professor in the Department of Pathological Neurochemistry, Institute of Molecular and Cellar Medicine, Okayama University Medical School, and then, in 1972, became professor and chairman of the Department of Neurochemistry, where he remained until he retired in 1995. Akitane continued his guanidino compound research there in Okayama, and developed distinctive analytical methods for guanidino compounds using automated liquid chromatography [10,11], fluorometry [12,13], or gas chromatography-mass spectrometry [14], and observed the above mentioned guanidino compounds. These analyses were used to elucidate the seizure mechanisms of these compounds as will be discussed in more detail in the following section [9,[15][16][17][18].…”

Increase of Methylguanidine and Guanidinoacetic Acid in the Brain of Amygdala‐Kindled Rats

“…The supernatant was passed through a column of Dowex 2 x 8 [Cl] (1.0 X 2 cm), and the colorless eluates were dried in vacuo and dissolved with 1 ml diluted HC1 (pH 2.2). Two hundred microliters of these samples was applied to high-performance liquid chromatography (HPLC) (Jasco G-520, Tokyo, Japan), and the phenanthrenequinon reaction method was used for detection (Yamamoto et al, 1978;Mori et al, 1979). Conditions of the guanidino compounds analysis were as follows: column, guanidino pack (6.0 x 50 mm, Jasco); column temperature, 70°C; flow rate of eluent, 1 .O ml/min; flow rate of 0.5% (wt/vol) 9,lO-phenanthrenequinon in dimethylformide, 0.5 ml/min; flow rate of 2 M sodium hydroxide, 0.5 ml/min; reaction coil, 0.5 mm Figure 1B shows the chromatogram of the guanidino compounds in the control rat AM.…”

“…Guanidino compounds are substances that have an amidino base and are known to be present in mammalian brain tissue (Mori et al, 1974(Mori et al, , 1978(Mori et al, , 1979Mizuno et al, 1975;, 1 9 7 6~; Marescau et al, 1986). These compounds have important biologic roles, e.g., the participation of arginine in ureagenesis (Krebs and Henseleit, 1932) and that of creatine in muscular contraction (Eggleton and Eggleton, 1927).…”