Fluorometric virus detection platform using quantum dots-gold nanocomposites optimizing the linker length variation

Nasrin, Fahmida; Chowdhury, Ankan Dutta; Takemura, Kenshin; Kozaki, Ikko; Honda, Hiroyuki; Adegoke, Oluwasesan; Park, Enoch Y.

doi:10.1016/j.aca.2020.02.039

Cited by 66 publications

(73 citation statements)

References 39 publications

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“…The possibility of tuning the emission spectra over a wide range of wavelengths and the resistivity to external physico-chemical conditions are also significant advantages [ 58 ]. Among them, fluorescent quantum dots (QD) have unique optical properties, which, in combination with the surface plasmon properties of metallic nanoparticles, can enhance the sensitivity of plasmonic biodetection systems [ 46 , 47 , 48 , 59 ]. Typically, most of quantum dots’ applications have been exploited in LSPR-based biosensors because the distance and dimensions of the adjacent gold nanoparticles can affect the fluorescence signal and, therefore, be quenched depending on the analyte concentration.…”

Section: Biosensing Strategiesmentioning

confidence: 99%

“…The enhancement of the fluorescent signal triggered by the LSPR signal from adjacent AuNPs allowed the sensitive detection of the antigens on the surface of H1N1 virus in deionized water (0.03 pg mL −1 ) and human serum (0.4 pg mL −1 ) ( Figure 7 ). The effect of the distance between fluorescent CdZnSeS/ZnSeS QDs and gold nanoparticles was studied by Nasrin et al using different lengths of peptide chains as a linkage [ 47 ]. The sensing mechanism was enhanced by the quenching of the QDs’ fluorescence due to the steric hindrance on the LSPR signal induced by the different concentrations of the influenza virus.…”

Section: Biosensing Strategiesmentioning

confidence: 99%

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Recent Progress in Plasmonic Biosensing Schemes for Virus Detection

Mauriz

2020

Sensors

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The global burden of coronavirus disease 2019 (COVID-19) to public health and global economy has stressed the need for rapid and simple diagnostic methods. From this perspective, plasmonic-based biosensing can manage the threat of infectious diseases by providing timely virus monitoring. In recent years, many plasmonics’ platforms have embraced the challenge of offering on-site strategies to complement traditional diagnostic methods relying on the polymerase chain reaction (PCR) and enzyme-linked immunosorbent assays (ELISA). This review compiled recent progress on the development of novel plasmonic sensing schemes for the effective control of virus-related diseases. A special focus was set on the utilization of plasmonic nanostructures in combination with other detection formats involving colorimetric, fluorescence, luminescence, or Raman scattering enhancement. The quantification of different viruses (e.g., hepatitis virus, influenza virus, norovirus, dengue virus, Ebola virus, Zika virus) with particular attention to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) was reviewed from the perspective of the biomarker and the biological receptor immobilized on the sensor chip. Technological limitations including selectivity, stability, and monitoring in biological matrices were also reviewed for different plasmonic-sensing approaches.

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Section: Biosensing Strategiesmentioning

confidence: 99%

Section: Biosensing Strategiesmentioning

confidence: 99%

Recent Progress in Plasmonic Biosensing Schemes for Virus Detection

Mauriz

2020

Sensors

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“…Fibre-optic LSPR biosensors [54,139] show a nearly 1000 fold higher sensitivity than LSPR sensors using aptamer functionalised gold nanodisk arrays (diameter= 100 nm, thickness = 20 nm, spacing = 200 to 30 nm) [55]. The sensitivity of virus detection has been reported to improve by seven orders of magnitude when AuNP (diameter = 26.5 ± 0.5 nm)-quantum dot (QD) nanoconjugates [147] are preferred over AgNPs (diameter = 20-80 nm) [148] as plasmonic nanomaterials owing to the oxidative degradation of AgNPs. Although signal enhancement of AuNPs for virus detection is lower in the case of nanohybrids using CdSeTeS QDs (size = 10.1 ± 2.9 nm) [137,149] or CdZnSeS QDs (size = 4.8 ± 0.6 nm) [147], a 33-fold lower LOD was reported when CdSeS QDs (size = 2.7-7.8 nm) were functionalised with a molecular beacon to obtain hairpin hybridisation assays [146].…”

Section: Localised Surface Plasmon Resonance Sensors For Biomedical Dmentioning

confidence: 99%

“…The sensitivity of virus detection has been reported to improve by seven orders of magnitude when AuNP (diameter = 26.5 ± 0.5 nm)-quantum dot (QD) nanoconjugates [147] are preferred over AgNPs (diameter = 20-80 nm) [148] as plasmonic nanomaterials owing to the oxidative degradation of AgNPs. Although signal enhancement of AuNPs for virus detection is lower in the case of nanohybrids using CdSeTeS QDs (size = 10.1 ± 2.9 nm) [137,149] or CdZnSeS QDs (size = 4.8 ± 0.6 nm) [147], a 33-fold lower LOD was reported when CdSeS QDs (size = 2.7-7.8 nm) were functionalised with a molecular beacon to obtain hairpin hybridisation assays [146]. Li's group established the superiority of LSPR over electrochemical sensing in the study of several neurotransmitters [117].…”

Section: Localised Surface Plasmon Resonance Sensors For Biomedical Dmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

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In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject. Optical sensors show versatility amongst different receptor types and even permit the integration of different detection mechanisms. Such conjugated sensing platforms facilitate the exploitation of their neoteric synergistic characteristics for sensor fabrication. This paper covers nearly 250 research articles since 2016 representing the emerging interest in rapid, reproducible and ultrasensitive assays in clinical analysis. Therefore, we present an elaborate review of biomedical diagnostics with the help of optical sensors working on varied principles such as surface plasmon resonance, localised surface plasmon resonance, evanescent wave fluorescence, bioluminescence and several others. These sensors are capable of investigating toxins, proteins, pathogens, disease biomarkers and whole cells in varied sensing media ranging from water to buffer to more complex environments such as serum, blood or urine. Hence, the recent trends discussed in this review hold enormous potential for the widespread use of optical sensors in early-stage disease prediction and point-of-care testing devices.

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“…At present, several kinds of virus detection tools can be found on the market based on biosensing techniques ( Baek et al, 2019 ; ( Nasrin et al, 2020 ), ( Simão et al, 2020 ), ( Kumar et al, 2020 ), ( Wu et al, 2020 ), ( Toldrà et al, 2020 ), ( Mukama et al, 2020 , p. 18), ( Huang et al, 2020 ), ( Faria and Zucolotto, 2019 ), ( Siuzdak et al, 2019 ). Moreover, FET based biosensor, enabling portable devices, is considered for real-time detection of the EBV antigen ( Chen et al, 2017 ), rotavirus ( Liu et al, 2013 ), and recently graphene-based FET has been designed to determine COVID-19 viral load in clinical nasopharyngeal samples, using a specific antibody against its spike protein ( Seo et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art

Khan¹,

Hasan

Hossain

et al. 2020

Biosensors and Bioelectronics

167

108

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Last few decades, viruses are a real menace to human safety. Therefore, the rapid identification of viruses should be one of the best ways to prevent an outbreak and important implications for medical healthcare. The recent outbreak of coronavirus disease (COVID-19) is an infectious disease caused by a newly discovered coronavirus which belongs to the single-stranded, positive-strand RNA viruses. The pandemic dimension spread of COVID-19 poses a severe threat to the health and lives of seven billion people worldwide. There is a growing urgency worldwide to establish a point-of-care device for the rapid detection of COVID-19 to prevent subsequent secondary spread. Therefore, the need for sensitive, selective, and rapid diagnostic devices plays a vital role in selecting appropriate treatments and to prevent the epidemics. During the last decade, electrochemical biosensors have emerged as reliable analytical devices and represent a new promising tool for the detection of different pathogenic viruses. This review summarizes the state of the art of different virus detection with currently available electrochemical detection methods. Moreover, this review discusses different fabrication techniques, detection principles, and applications of various virus biosensors. Future research also looks at the use of electrochemical biosensors regarding a potential detection kit for the rapid identification of the COVID-19.

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Fluorometric virus detection platform using quantum dots-gold nanocomposites optimizing the linker length variation

Cited by 66 publications

References 39 publications

Recent Progress in Plasmonic Biosensing Schemes for Virus Detection

Recent Progress in Plasmonic Biosensing Schemes for Virus Detection

Recent Progress in Optical Sensors for Biomedical Diagnostics

Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art

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