Fluorometric micro determination of citrate in tissue extracts

O’Neill, J. J.; Sakamoto, Tetsuhiko

doi:10.1016/0003-2697(69)90322-4

Analytical Biochemistry

1969

DOI: 10.1016/0003-2697(69)90322-4

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Fluorometric micro determination of citrate in tissue extracts

J. J. O’Neill

Tetsuhiko Sakamoto

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Cited by 7 publications

References 15 publications

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Measurement of choline acetyltransferase with [14C]acetate by a cycling procedure

O’Neill

Hruschak

1987

Neurochem Res

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A multiple enzyme and multisubstrate cycling system is described for the radiometric determination of cholineacetyltransferase (ChAT) activity in crude tissue homogenates. The methods employs [14C]acetate coupled with the enzymes acetate kinase (AK) and phosphotransacetylase (PTA) for the generation of [14C]acetyl CoA. By recycling it was possible to avoid product inhibition of ChAT by CoA, ATP was maintained constant by rephosphorylation of ADP. Kinetics of the individual enzyme reactions were studied and the parameters obtained were used to select appropriate conditions to maintain linearity of varying amounts ChAT activity over a sixty minute time course. The sensitivity of the method is limited only by the specific activity of commercially available isotope labeled acetate.

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Measurement of choline acetyltransferase with [14C]acetate by a cycling procedure

O’Neill

Hruschak

1987

Neurochem Res

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Enzymatic and electron microscopic analysis of isolated osteoclasts

Walker

1972

Calc. Tis Res.

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Rapid and accurate determination of malate, citrate, pyruvate and oxaloacetate by enzymatic reactions coupled to formation of a fluorochromophore: Application in colorful juices and fermentable food (yogurt, wine) analysis

Shapiro

Silanikove

2011

Food Chemistry

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Fluorometric micro determination of citrate in tissue extracts

Cited by 7 publications

References 15 publications

Measurement of choline acetyltransferase with [14C]acetate by a cycling procedure

Measurement of choline acetyltransferase with [14C]acetate by a cycling procedure

Enzymatic and electron microscopic analysis of isolated osteoclasts

Rapid and accurate determination of malate, citrate, pyruvate and oxaloacetate by enzymatic reactions coupled to formation of a fluorochromophore: Application in colorful juices and fermentable food (yogurt, wine) analysis

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