Fluorometric estimation of triglycerides in serum by a modification of the method of Bucolo and David.

Abstract

An enzymic, flurometric method is described for determination of triglycerides (and glycerol) in blood serum, a modification of the method of Bucolo and David [Clin. Chem. 19, 476 (1973)]. Commercially available reagent kits are used. The rate of disappearance of NADH fluorescence at 460 nm (excitation wavelength, 365 nm) is monitored and related to serum triglyceride concentration, corrected for the content of free glycerol. We compared the results obtained fluorometrically to the ultraviolet spectrophotometric Boehringer Neutral Fat method used at Gentofte Hospital, Copenhagen. Instrument response and concentration were linearly related in the range 0.27 to 2.7 mmol of triglycerides per liter of serum with the fluorometric method. The CV was 0.9% for the fluorometric method, 3.7% for the spectrophotometric procedure. The fluorometric method requires less reagents, time, and calculations than does the spectrophotometric method.

Combination of a high-fat diet with sweetened condensed milk exacerbates inflammation and insulin resistance induced by each separately in mice

“…The rate constant for the ITT (kITT) was calculated using the formula kITT (%/min) = 0.693/t½, where t½ is calculated from the slope of the plasma glucose concentration during the period from 0 to 20 minutes after insulin injection, using least squares analysis; the decline in plasma glucose concentration during this period was linear. Total cholesterol 48 , triacylglycerol 49 and the activity of aspartate aminotransferase-AST 50 were evaluated using colorimetric assays (Labtest Diagnostics, Lagoa Santa, MG, Brazil), and insulin was determined using ELISAs (Millipore kit, St. Charles, MO). LDL-cholesterol was calculated using the Friedewald equation 51 .…”

Synthesis, Docking and Biological Activities of Novel Hybrids Celecoxib and Anthraquinone Analogs as Potent Cytotoxic Agents

“…The biochemical effects of the selected compounds, on some liver enzymes such as aspartate, alanine aminotransferases (AST and ALT) [ 28 ] and alkaline phosphatase (ALP) [ 29 ], were analyzed using a blood auto analyzer (Olympus AV 400, Tokyo, Japan). Moreover, albumin [ 30 ], globulins [ 31 ], creatinine [ 32 ], total lipids [ 33 ], cholesterol [ 34 ], triglycerides and bilirubin [ 35 ] in serum of mice were evaluated in comparison to 5-FU and DOX. Statistical analysis of the results was performed using Chi-square values (SPSS computer program, IBM Corporation, NY, USA).…”